Bacterial factors influencing adhesion of Pseudomonas aeruginosa strains to a poly(ethylene oxide) brush

Roosjen, Astrid; Busscher, Henk J.; Norde, Willem; Mei, Henny C. van der

doi:10.1099/mic.0.29005-0

Cited by 98 publications

(87 citation statements)

References 41 publications

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“…When salon makeup brush samples were analyzed for microbial contamination it was seen that for Staphylococcus aureus CFU/ml was 22. (ethylene oxide)-PEO brush coatings very well [13]. In the wax samples the estimated contamination of Staphylococcus aureus was 25×10 3 CFU/ml.…”

Section: Resultsmentioning

confidence: 79%

Incidence of Human Skin Pathogens from Cosmetic Tools used in Beauty Saloons in Different Areas of Lahore, Pakistan

et al. 2012

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With the surprising development across the cosmetic and personal care companies the reutilization of cosmetic tools is of a common practice. Isolation and detection of human skin pathogens from 100 samples of beauty salon tools i.e., blusher brush, face sponge and wax has been done. All the samples were examined microbiologically for the contamination of Staphylococcus aureus, Pseudomonas aeruginosa, yeast and fungus. It was observed that the percentage of Staphylococcus aureus was higher (100% in sponge, 100% in brush, 88% in wax) in the tools than Pseudomonas aeruginosa (69.6% in sponge, 81.8% in brush and 73.5% in wax), where counts obtained for fungus was 51.5% in sponge, 30.3% in brush and 20.5% in wax. It was observed that the major cause of contamination of saloon tools is repetitive usage on all costumers without considering the hygienic conditions.

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Section: Resultsmentioning

confidence: 79%

Incidence of Human Skin Pathogens from Cosmetic Tools used in Beauty Saloons in Different Areas of Lahore, Pakistan

et al. 2012

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“…In addition to PTFE, PP also possessed a hydrophobic surface, whereas the remaining support materials had hydrophilic surfaces. [20,29]. In this study, M. barkeri also possessed a more energetic surface than the support materials, with a total surface free energy of 57 mJ.m -2 .…”

Section: Contact Angle Measurementmentioning

confidence: 90%

Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material

Nguyen

Karunakaran

Collins

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can't change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/ Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. *Graphical Abstract HighlightsOf the six materials tested Methanosarcina barkeri selectively adheres to polytetrafluoroethylene (PTFE), polypropylene (PP) and polyvinyl chloride (PVC) The best support materials for initial adhesion after 2 h also performed best after 72 h. xDLVO model predictions correlated with experimental adhesion results. Applicability of the xDLVO model in identifying support materials for selective attachment of M. barkeri. ! *Highlights (for review) Physicochemical analysis of initial adhesion and biofilm formation of HighlightsOf the six materials tested Methanosarcina barkeri selectively adheres to polytetrafluoroethylene (PTFE), polypropylene (PP) and polyvinyl chloride (PVC) The best support materials for initial adhesion after 2 h also performed best after 72 h. xDLVO model predictions correlated with experimental adhesion results. Applicability of the xDLVO model in identifying support materials for selective attachment of M. barkeri. AbstractThe retention of selective biofilms of Methanosarcina species within anaerobic digesters could reduce start-up times and enhance the efficiency of the process in treating high-strength domestic sewage. The objective of the study was to examine the effect of the surface characteristics of six common polymer support materials on the initial adhesion of the model methanogen, Methanosarcina barkeri, and to assess the potential of these support materials as selective biofilm carriers. Results from both the initial adhesion tests and extended DLVO (xDLVO) model correlated with each other, with PVC (12% surface coverage/mm 2 ), PTFE (6% surface coverage/mm 2 ), and PP (6% surface coverage/mm 2 ), shown to be the better performing support materials for initial adhesion, as well as subsequent biofilm formation by M. barkeri after 72 h. These three support materials Experimental results showed that the type of material strongly influenced the extent of adhesion from M. barkeri (p < 0.0001), and the xDLVO model was able to explain the results in these environmental conditions. Therefore, DLVO physicochemical forces were found to be influential on the initial adhesion of M. barkeri. Scanning electron microscopy suggested that production of extracellular polymeric substances ...

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“…Hydrophobic interactions between the brush layer and approaching nonmotile cells can develop. The hydrophobic characteristic of the cell surface was previously shown to govern the deposition of P. aeruginosa cells onto a poly(ethylene oxide) brush layer (36).…”

Section: Resultsmentioning

confidence: 99%

Impact of Alginate Conditioning Film on Deposition Kinetics of Motile and Nonmotile Pseudomonas aeruginosa Strains

Kerchove

Elimelech

2007

Appl Environ Microbiol

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The initial deposition of bacteria in most aquatic systems is affected by the presence of a conditioning film adsorbed at the liquid-solid interface. Due to the inherent complexity of such films, their impact on bacterial deposition remains poorly defined. The aim of this study was to gain a better understanding of the effect of a conditioning film on the deposition of motile and nonmotile Pseudomonas aeruginosa cells in a radial stagnation point flow system. A well-defined alginate film was used as a model conditioning film because of its polysaccharide and polyelectrolyte nature. Deposition experiments under favorable (nonrepulsive) conditions demonstrated the importance of swimming motility for cell transport towards the substrate. The impact of the flagella of motile cells on deposition is dependent on the presence of the conditioning film. We showed that on a clean substrate surface, electrostatic repulsion governs bacterial deposition and the presence of flagella increases cell deposition. However, our results suggest that steric interactions between flagella and extended polyelectrolytes of the conditioning film hinder cell deposition. At a high ionic strength (100 mM), active swimming motility and changes in alginate film structure suppressed the steric barrier and allowed conditions favorable for deposition. We demonstrated that bacterial deposition is highly influenced by cell motility and the structure of the conditioning film, which are both dependent on ionic strength.Biofilm formation or biofouling, a widespread problem in aquatic environments, can negatively affect processes in natural, engineered, and biomedical systems, resulting in contaminated aquifers (25), fouled membranes (3), and infected catheters and biomedical implants (40). The accumulation of metabolically active microorganisms on surfaces can lead to material degradation and affect system performance through energy cost increases and reduction in expected life spans. Biofouling control remains a major challenge because of the intricate processes involved in biofilm development, such as bacterial deposition, growth, and maturation (10). A better understanding of bacterial deposition-the step that initiates biofouling-can be used to develop improved control and prevention strategies in order to reduce the adverse impacts of biofilms on aquatic environments.Most fundamental studies have investigated the initial deposition of microbes in oversimplified systems using ultraclean surfaces as a surrogate for the solid-liquid interface (19,21). However, the properties of the solid-liquid interface are altered by the adsorption of polyelectrolytes, such as humic substances and polysaccharides in natural aquatic systems (31) and glycoproteins, lipids, and nucleotides in biomedical systems (1). Because of its charged and macromolecular nature, this polyelectrolyte film, known as the conditioning film, changes the physicochemical properties of the surface (37) (e.g., surface roughness and surface charge distribution), which affects bacterial depo...

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Bacterial factors influencing adhesion of Pseudomonas aeruginosa strains to a poly(ethylene oxide) brush

Cited by 98 publications

References 41 publications

Incidence of Human Skin Pathogens from Cosmetic Tools used in Beauty Saloons in Different Areas of Lahore, Pakistan

Incidence of Human Skin Pathogens from Cosmetic Tools used in Beauty Saloons in Different Areas of Lahore, Pakistan

Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material

Impact of Alginate Conditioning Film on Deposition Kinetics of Motile and Nonmotile Pseudomonas aeruginosa Strains

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