Bacterial exopolysaccharide (EPS)-coated ZnO nanoparticles showed high antibiofilm activity and larvicidal toxicity against malaria and Zika virus vectors

Abinaya, M.; Vaseeharan, Baskaralingam; Divya, Mani; Sharmili, Aruna; Govindarajan, Marimuthu; Alharbi, Naiyf S.; Kadaikunnan, Shine; Khaled, Jamal M.; Benelli, Giovanni

doi:10.1016/j.jtemb.2017.10.002

Cited by 159 publications

(41 citation statements)

References 52 publications

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“…These morphological changes seem to be responsible for the larvicidal toxicity by LB extract and LB-ZnO NRs. Similar morphological damages were reported by Abinaya et al [72] when Ae. aegypti larvae were treated with bacterial exopolysaccharide (EPS)-coated ZnO nanoparticles.…”

Section: Mosquito Larvicidal Activitysupporting

confidence: 90%

Eco-friendly approach to control dengue vector Aedes aegypti larvae with their enzyme modulation by Lumnitzera racemosa fabricated zinc oxide nanorods

Dhavan

Jadhav

2020

SN Appl. Sci.

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Aedes aegypti mosquito species is a primary vector for dengue, chikungunya and Zika infections, and vector control is the only key approach for reducing their transmission. The present study emphasizes on environmental friendly approach for the fabrication of zinc oxide nanorods (ZnO NRs) using aqueous extract of Lumnitzera racemosa flower buds (LB) as a reducing and stabilizing agent. ZnO NRs were examined by UV-Vis spectroscopy with characteristic absorbance band at 373.82 nm and bandgap of 3.25 eV. FT-IR analysis revealed the functional groups associated with ZnO NRs. The crystallinity of LB-ZnO NRs was further investigated using X-ray diffraction. The Zeta potential displayed a surface charge of − 23.5 mV on NRs. Energy dispersive spectra analysis and SEM analysis confirmed the formation of ZnO NRs whilst TEM highlighted the average length and diameter in the range of 250-300 nm and 45-50 nm respectively. LB-ZnO NRs were found to be efficacious against Ae. aegypti 4th instar larvae with LC 50 of 24.74 μg/ml. Decreased acetylcholinesterase (60.6%) and glutathione S-transferase (24.4%) activities were also evaluated in Ae. aegypti larvae which were exposed to synthesized LB-ZnO NRs with no genetic aberrations. All these outcomes propose the potential employment of LB-ZnO NRs in mosquito control, as well as an effective alternative to insecticide resistance.

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Section: Mosquito Larvicidal Activitysupporting

confidence: 90%

Eco-friendly approach to control dengue vector Aedes aegypti larvae with their enzyme modulation by Lumnitzera racemosa fabricated zinc oxide nanorods

Dhavan

Jadhav

2020

SN Appl. Sci.

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“…It is important to that note other bacterial EPSs have been used as reducing and stabilizing agents such is the case of EPSs obtained from Bacillus licheniformis Dahb1 used to synthetize ZnO nanoparticles by the coprecipitation method [102]. These nanoparticles presented effective antibacterial and antibiofilm activity against both gram negative and gram positive bacteria, as well as antifungal and larvicidal activity with low cytotoxicity in hemolytic assays.…”

Section: Role Of Bacterial Epss In the Synthesis Of Other Metal Nanopmentioning

confidence: 99%

“…In addition, when magnetic nanoparticles are produced, vibrating sample magnetometry (VSM) and superconducting quantum interference device (SQUID) magnetometry are adequate techniques to measure magnetic properties [130]. In relation to biomedical measurements carried out during biomedical applications of metal nanoparticles synthesized by bacterial EPSs, the antimicrobial activity of metal nanoparticles synthesized by bacterial EPSs has been measured by determination of minimum inhibitory concentration (MIC) [85,86,102,120], minimum bactericidal concentration (MBC) [86,120], fluorescence microscopy imaging (FMI) [120], skin infection model in vivo by colony counting method using the pour plate technique [120], agar disc diffusion method [53,70,72,90,91], agar well diffusion method [33,85,86,88,[90][91][92]102], colony counting method [76], antibiofilm activity [85,102], bacterial growth kinetics tests, and kill time assays [85,86,92,102]. Moreover, antifungal activity was measured by agar well and disk diffusion method [72,102] as well as larvicidal activity using the World Health Organization method [102,131].…”

Section: Main Techniques Used To Characterize Metalmentioning

confidence: 99%

Bacterial Exopolysaccharides as Reducing and/or Stabilizing Agents during Synthesis of Metal Nanoparticles with Biomedical Applications

Escárcega‐González

Garza-Cervantes

Vázquez-Rodríguez

et al. 2018

International Journal of Polymer Science

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Bacterial exopolysaccharides (EPSs) are biomolecules secreted in the extracellular space and have diverse biological functionalities, such as environmental protection, surface adherence, and cellular interactions. EPSs have been found to be biocompatible and eco-friendly, therefore making them suitable for applications in many areas of study and various industrial products. Recently, synthesis and stabilization of metal nanoparticles have been of interest because their usefulness for many biomedical applications, such as antimicrobials, anticancer drugs, antioxidants, drug delivery systems, chemical sensors, contrast agents, and as catalysts. In this context, bacterial EPSs have been explored as agents to aid in a greener production of a myriad of metal nanoparticles, since they have the ability to reduce metal ions to form nanoparticles and stabilize them acting as capping agents. In addition, by incorporating EPS to the metal nanoparticles, the EPS confers them biocompatibility. Thus, the present review describes the main bacterial EPS utilized in the synthesis and stabilization of metal nanoparticles, the mechanisms involved in this process, and the different applications of these nanoparticles, emphasizing in their biomedical applications.

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“…Microorganisms produced their metabolites are called as bioflocculant during their growth. Non-toxic nature of the bioflocculant are alternative to synthetic flocculant involving in bioremediation and biomedical application 1,2 . Several bacterial species are capable of producing bioflocculant like Alcaligenes latus, Bacillus firmus 3 , Aspergillus sp.…”

Section: Introductionmentioning

confidence: 99%

Role of Bacterial Bioflocculant on Antibiofilm Activity and Metal Removal Efficiency

Vimala¹

2019

J Pure Appl Microbiol

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In the present study, bioflocculant derived from bacteria are widely explored in bioremediation such as removing heavy metal and inhibiting biofilm formation. The bacteria isolated from sewage identified as Proteus mirabilis using 16S rRNA gene made up of D-Mannose, D-Fructose, D-Sucrose, and D-Galactose could be identified by Gas liquid Chromatography Mass Spectrometry (GCMS). Fourier transform infrared (FTIR) analysis of the bioflocculant indicated the presence of-COOH,-OH, and-NH groups which showed the characteristic of polysaccharide and protein. The removing percentage of heavy metals achieved by the precipitated bioflocculant were 82.63 ± 1.20, 72.076 ± 0.42, 57.36 ± 1.05, and 44.7 ± 1.053%, respectively for Zn 2+ , Cd 2+ , Cu 2+ , and Hg 2+ at optimum conditions. Bioflocculant were exhibited excellent biofilm inhibition against P. aeruginosa where maximum reduction (73%) were achieved at the minimum amount of 80µg/ml dose. Similarly, maximum reduction (80%) were achieved at the minimum amount of 70µg/ml dose for S. aureus. It was concluded that the bioflocculant produced have the potential in metal removal and biofilm inhibition.

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Bacterial exopolysaccharide (EPS)-coated ZnO nanoparticles showed high antibiofilm activity and larvicidal toxicity against malaria and Zika virus vectors

Cited by 159 publications

References 52 publications

Eco-friendly approach to control dengue vector Aedes aegypti larvae with their enzyme modulation by Lumnitzera racemosa fabricated zinc oxide nanorods

Eco-friendly approach to control dengue vector Aedes aegypti larvae with their enzyme modulation by Lumnitzera racemosa fabricated zinc oxide nanorods

Bacterial Exopolysaccharides as Reducing and/or Stabilizing Agents during Synthesis of Metal Nanoparticles with Biomedical Applications

Role of Bacterial Bioflocculant on Antibiofilm Activity and Metal Removal Efficiency

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