Bacterial Diversity in Mine Tailings Compared by Cultivation and Cultivation-independent Methods and their Resistance to Lead and Cadmium

Abstract

To examine bacterial community composition in rhizosphere of plants colonizing on mine tailings and phylogenetic differences between subcommunities resistant to different metals, we constructed four clone libraries of 16S rDNA sequences. One was amplified directly from tailing microbial DNA (named as Ci library) and three from cultures on the plates containing of 0.5 mM CdCl(2) (Cd library), 2 mM Pb (NO(3))(2) (Pb library), and without any metals (Cw library). In total, nine bacterial divisions and two unclassified groups were identified from 352 clones of these libraries. Ci clones covered eight divisions, whereas all cultivable clones only covered four divisions. Thus, Ci library provided more phylogenetic diversity than cultivable libraries. However, the microbes represented by the cultivable clones were more similar to previously described bacteria than those represented by Ci clones. All Ci clones were not found in three cultivable libraries. Cd library were exclusively Gram-negative bacteria of Acinetobacter, Ralstonia, Comamonas, and Chryseobacterium. Meanwhile, dominant Gram-positive bacteria in Pb library, Paenibacillus and Bacillus, were also not found in Cd library. Our data indicate that phylogenetic structure was very different from those in acid mine drainage. Meanwhile, tailings harbored phylogenetically distinct subcommunities resistant to Pb and Cd.

Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study

“…Comparisons of 13k and 19k 16S rDNA sequence against a RDP database placed both isolates in the genus Bacillus, which agrees with the data of others examining mine waste [25]. Isolate 13k exhibited 100% identity with B. simplex isolates, but also with a B. muralis and a [27,28] in the phylogenetic analysis (Fig.…”

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

“…Studies on the relationship between mining and microbial communities in surrounding soils mainly focused on nonferrous metals (e.g., Cu, Pb, and Sb), which are highly toxic to soil biota (Zhang et al 2007). However, few studies have focused on iron mining despite its specific environmental impact.…”

Safety Assessment of Transgenic Organisms, Volume 4

“…The fertilised ovule develops into a seed, the epidermis of which gives rise to many single-celled seed hairs (epidermal trichomes) of two kinds: long hairs referred to as "lint" and coarser short hairs referred to as "fuzz" or "linters" (Applequist et al, 2001;Zhang et al, 2007). Differentiation between the hair types has been maximised to produce the white lint in elite cultivars.…”

“…The capsular fruit, which splits open at maturity, is referred to as the "boll"; it is spherical or ovoid with a beak at the top, and differs among species in shape, size and colour. Gossypium hirsutum bolls are usually of a pale-green colour and relatively smooth with few punctate gossypol glands; those of G. barbadense are of darker green and conspicuously pitted with numerous glands.The fertilised ovule develops into a seed, the epidermis of which gives rise to many single-celled seed hairs (epidermal trichomes) of two kinds: long hairs referred to as "lint" and coarser short hairs referred to as "fuzz" or "linters" (Applequist et al, 2001;Zhang et al, 2007). Differentiation between the hair types has been maximised to produce the white lint in elite cultivars.…”