Bacterial cellulose production from acerola industrial waste using isolated kombucha strain

Abstract

Bacterial Cellulose (BC) production is still considered expensive and challenging for industries. Herein, BC was produced through an acetic acid bacteria isolated from the kombucha consortium and an extract from acerola juice-industrial waste. The isolated bacterium was characterized through different assays (biochemical characterization and 16S rRNA gene) being identi ed as Komagataeibacter rhaeticus. BC production with static cultivation mode by the isolated strain was compared using traditional Hestrin-Schramm (HS) medium and acerola waste (AC) (5% w/v). The kinetic behavior of BC production was slightly higher in the HS medium reaching 2.9 g/L after 12 days of fermentation, while 2.3 g/L in the AC medium. Minor differences were observed between crystallite size and d-spacing, highlighting BC produced by the AC medium with higher crystallinity of 93.9% and two-fold breaking stress resistance in comparison with the conventional medium, with high-temperature stability and economically feasible, promissory results for further application of this synthetized cellulose obtained from industrial residues.

Comparative Study and Characterization of Water-treated Bacterial Cellulose produced by Solid or Liquid inoculum of Komagateibacter sucrofermentans

“…In the study of He, also, was veri ed a higher drop in the pH value in 2 days of Komagataeibacter rhaeticus cultivation and the pH value in the HS medium after 7 days reached at 3.6 (He et al 2020). According to Leonarski, the higher drop in the pH value was in 2 days of Komagataeibacter strain cultivation and the pH value in the HS medium after 7 days reached around 3.5 (Leonarski et al 2022). Additionally, increasing the volume of the liquid inoculum or the amount of the solid inoculum of K. sucrofermentans proportionally in HS the medium, pH was ranged at 3.53-3.71 and 3.68-3.70, respectively (Table 2).…”

“…The difference between two celluloses (Iα or Iβ) can be identi ed with the help of the Z values. More analytically, the Z value is a parameter used to discriminate whether BC is enriched in Iα or in Iβ type in which Z > 0 means BC is a Iα-rich type (known as triclinic) and Z < 0 a Iß-dominant type (known as monoclinic) (Leonarski et al 2022). According to the results in Table 1, where the value of Z is greater than 0, all BC pellicles (BCL…”

“…Cellulose is described as a two-region combination: crystalline (ordered) and amorphous (less ordered) regions, due to its supramolecular structure. Thus, cellulose can be considered as semicrystalline polymer that in its original or ''native" state presents polymorphism I with a mixture of two allomorphs Iα and Iβ and Iα/Iβ ratio which varies according to the biological source and culture methodology (Trache et al 2016;Leonarski et al 2022). The values of Z, interplanar distances (d-spacing), crystallite size (CS), and crystallinity index (CI) are shown in Table 1.…”

Bacterial Cellulose Applied in Wound Dressing Materials: Production and Functional Modification – A Review

“…During the exploration of wound dressings, bacterial cellulose (BC) has gradually become one of the focal points due to its ideal characteristics. Since the first report of BC, [ 3 ] researchers have successively discovered a series of BC‐producing strains, such as Gluconacetobacter , [ 4 ] Acetobacter , [ 5 ] Komagataeibacter , [ 6 ] Agrobacterium , [ 7 ] Aerobacter , [ 8 ] Azotobacter , [ 9 ] Achromobacter , [ 10 ] Rhizobium , [ 11 ] Salmonella , [ 12 ] and some Gram‐positive bacteria. [ 13 ] BC is a linear polymer composed of β‐ d ‐glucose linked by β‐1,4‐glycosidic bonds, and its chemical structure is similar to that of plant cellulose.…”