Bacterial Aerosol Samplers

Tyler, M. E.; Shipe, E. L.

doi:10.1128/aem.7.6.337-349.1959

Cited by 31 publications

(2 citation statements)

References 19 publications

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“…Sampling. Aerosol samples were collected by means of all glass impingers (AGI), described previously by Tyler and Shipe (1959). To these were attached preimpingers which in general collect particles greater than 5 ,u and allow particles less than 5 ,u to pass into the AGI (May and Druett, 1953).…”

Section: Physical Tracers For Bacterial Aerosolsmentioning

confidence: 99%

Physical Tracers for Bacterial Aerosols

Miller¹,

Scherff²,

Piepoli³

et al. 1961

Applied Microbiology

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Section: Physical Tracers For Bacterial Aerosolsmentioning

confidence: 99%

Physical Tracers for Bacterial Aerosols

Miller¹,

Scherff²,

Piepoli³

et al. 1961

Applied Microbiology

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“…In addition, a single sampling solution can be plated onto a variety of media plus subjected to chemical tests or toxin analyses. Limitations of the AGI were noted as loss of viability due to impingement of the microorganisms (45,49,50) and loss by re-entrainment in the exhaust flow caused by hydrophobicity, agitation, and misting within the impinger (38). In addition, some organisms may suffer from the effects of sudden hydration upon impingement or osmotic shock (6).…”

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Comparison of bioaerosol sampling methods in barns housing swine

Thorne

Kiekhaefer

Whitten

et al. 1992

Appl Environ Microbiol

158

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The air in livestock buildings contains bioaerosol levels that are sufficiently high to cause adverse health effects in animals and workers. These bioaerosols are complex mixtures of live and dead microorganisms and their products as well as other aeroallergens. The effectiveness of sampling methods used for quantifying the very high concentrations of microorganisms in these environments has not been well studied. To facilitate an accurate assessment of respiratory hazards from viable organisms in agricultural environments, three bioaerosol sampling methods were investigated: the Andersen microbial sampler method (AMS), the all-glass impinger method (AGI), and the Nuclepore filtration-elution method (NFE). These methods were studied in a parallel fashion in 24 swine confinement buildings. Measurements were taken in two seasons with three types of culture media in duplicate to assess total bacteria, gram-negative enteric bacteria, and total fungi. Methods were analyzed for the proportion of samples yielding data within the limits of detection, intraclass reliability, and correlation between methods. For sampling viable bacteria, the AMS had a poor data yield because of overloading and demonstrated weak correlation with the AGI. Conversely, the AGI and NFE gave sufficient numbers of valid data points (90%o), yielded high intraclass reliabilities (at 0.92), and were highly correlated with each other (r = 0.86). The AGI and the NFE were suitable methods for assessing bacteria in this environment, but the AMS was not. The AMS was the only method that consistently recovered enteric bacteria (73% data yield). For sampling fungi, the AGI and AMS both yielded sufficient data and all three methods demonstrated high intraclass reliability. The AGI and AMS correlated moderately with each other, but each correlated well with the NFE. However, the AGI measured significantly higher airborne fungal concentrations than did the AMS. Thus, the AGI was the preferred sampling method for viable fungi. Selection of an appropriate method depends on the purpose of sampling, expected bioaerosol concentrations, and environmental conditions. In this study, the AGI was best for total bacteria and fungi and the AMS was preferred for sampling enteric organisms.

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