Bioaerosol Sampling and Analysis

Buttner, Mark P.

doi:10.1002/0471263397.env015

Cited by 2 publications

(2 citation statements)

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“…Moreover, the efficiency in bioaerosol collection is usually very low with the method. In the case of the instrumental sampling methods, bioaerosols are collected based on several principles of operations, such as collection into swirling liquids, filtration by porous membranes, and impaction onto culture media . Among them, air filtration technique using filter media and electrostatic precipitators represent the mostly used instrumental sampling methods.…”

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“…In the case of the instrumental sampling methods, bioaerosols are collected based on several principles of operations, such as collection into swirling liquids, filtration by porous membranes, and impaction onto culture media. 32 Among them, air filtration technique using filter media and electrostatic precipitators represent the mostly used instrumental sampling methods. However, large amount of water is typically needed to transfer the captured bioaerosols into aqueous media, making the target overly diluted for direct bioanalysis.…”

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Microfluidic Air Sampler for Highly Efficient Bacterial Aerosol Collection and Identification

Bian

Wang

et al. 2016

Anal. Chem.

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The early warning capability of the presence of biological aerosol threats is an urgent demand in ensuing civilian and military safety. Efficient and rapid air sample collection in relevant indoor or outdoor environment is a key step for subsequent analysis of airborne microorganisms. Herein, we report a portable battery-powered sampler that is capable of highly efficient bioaerosol collection. The essential module of the sampler is a polydimethylsiloxane (PDMS) microfluidic chip, which consisted of a 3-loop double-spiral microchannel featuring embedded herringbone and sawtooth wave-shaped structures. Vibrio parahemolyticus (V. parahemolyticus) as a model microorganism, was initially employed to validate the bioaerosol collection performance of the device. Results showed that the sampling efficacy reached as high as >99.9%. The microfluidic sampler showed greatly improved capturing efficiency compared with traditional plate sedimentation methods. The high performance of our device was attributed to the horizontal inertial centrifugal force and the vertical turbulence applied to airflow during sampling. The centrifugation field and turbulence were generated by the specially designed herringbone structures when air circulated in the double-spiral microchannel. The sawtooth wave-shaped microstructure created larger specific surface area for accommodating more aerosols. Furthermore, a mixture of bacterial aerosols formed by V. parahemolyticus, Listeria monocytogenes, and Escherichia coli was extracted by the microfluidic sampler. Subsequent integration with mass spectrometry conveniently identified the multiple bacterial species captured by the sampler. Our developed stand-alone and cable-free sampler shows clear advantages comparing with conventional strategies, including portability, easy-to-use, and low cost, indicating great potential in future field applications.

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confidence: 99%