Specific Detection of Fungi Associated With SBS When Using Quantitative Polymerase Chain Reaction

Cruz, Patricia; Stetzenbach, Linda D.

doi:10.1016/s0065-2164(04)55018-9

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…Currently, polymerase chain reaction (PCR) methods are used in which the target DNA from building material is used as a template. In quantitative PCR (qPCR), quantitative data on the presence of viable and dead molds can be obtained—information that is not possible to obtain with the present culture methods (Cruz and Stetzenbach 2004; Meklin et al 2004). These new methods are not yet fully developed and need to be evaluated (Keswani et al 2005; McDevitt et al 2004; Vesper et al 2004).…”

Section: Exposure Assessmentmentioning

confidence: 99%

Current State of the Science: Health Effects and Indoor Environmental Quality

Mitchell

Zhang²,

Sigsgaard

et al. 2007

Environ Health Perspect

207

119

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Our understanding of the relationship between human health and the indoor environment continues to evolve. Previous research on health and indoor environments has tended to concentrate on discrete pollutant sources and exposures and on specific disease processes. Recently, efforts have been made to characterize more fully the complex interactions between the health of occupants and the interior spaces they inhabit. In this article we review recent advances in source characterization, exposure assessment, health effects associated with indoor exposures, and intervention research related to indoor environments. Advances in source characterization include a better understanding of how chemicals are transported and processed within spaces and the role that other factors such as lighting and building design may play in determining health. Efforts are under way to improve our ability to measure exposures, but this remains a challenge, particularly for biological agents. Researchers are also examining the effects of multiple exposures as well as the effects of exposures on vulnerable populations such as children and the elderly. In addition, a number of investigators are also studying the effects of modifying building design, materials, and operations on occupant health. Identification of research priorities should include input from building designers, operators, and the public health community.

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Section: Exposure Assessmentmentioning

confidence: 99%

Current State of the Science: Health Effects and Indoor Environmental Quality

Mitchell

Zhang²,

Sigsgaard

et al. 2007

Environ Health Perspect

207

119

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Analysis of Bioaerosol Samples

Cruz¹,

Buttner²

2007

Manual of Environmental Microbiology

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Análise Da Qualidade Do Ar Na Biblioteca Pública De Um Campus Universitário

Sousa¹,

Pantoja²,

Nunes³

2016

Anais Do XIV Encontro Nacional De Estudantes De Engenharia Ambiental

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Resumo: Os fungos são ditos anemófilos quando dispersos por via atmosférica e são causadores de doenças alérgicas. O objetivo da presente pesquisa foi analisar a qualidade do ar de uma biblioteca pública universitária, no município de Fortaleza-CE, através de identificação da microbiota fúngica. Para tanto, analisou-se 4 setores, a saber: Recepção, Acervo Geral, Sala de Estudo Coletivo e Administração. Foram realizadas coletas mensais em cada setor, através do método da sedimentação passiva em placas de Petri, contendo o meio de cultura Ágar Batata Dextrose (Himedia®). Cada placa ficou exposta por 8 horas. Findo o período de coleta, as amostras vedadas seguiram para o laboratório, onde permaneceram incubadas a temperatura ambiente durante sete dias, realizando-se observações diárias. A partir do aparecimento de colônias fúngicas procedeu-se a contagem global e identificação das mesmas através de análise macro e micromorfológica. No total de 36 placas expostas, isoladas 187 colônias e identificados 4942 unidades formadoras de colônias com 25 gêneros fúngicos. A sequência dos locais mais contaminados foi: Recepção, Acervo, Sala de Estudo Coletivo e Administração. No geral, foram verificados importantes fungos desencadeadores de alergias respiratórias, como: Aspergillus sp., Penicillium sp., Alternaria sp. e Cladosporium sp. Um dos gêneros de maior expressão foi a Chrysonilia sp, patógeno vegetal que potencializa a degradação do acervo presente. Por fim, pondera-se que diferentes ambientes de uma biblioteca podem ser insalubres, pois possui grande quantidade de fungos anemófilos que podem desencadear alergias respiratórias nos frequentadores sendo assim necessários mais estudos do caso e monitoramento periódico do ar na biblioteca.

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Specific Detection of Fungi Associated With SBS When Using Quantitative Polymerase Chain Reaction

Cited by 3 publications

References 34 publications

Current State of the Science: Health Effects and Indoor Environmental Quality

Current State of the Science: Health Effects and Indoor Environmental Quality

Analysis of Bioaerosol Samples

Análise Da Qualidade Do Ar Na Biblioteca Pública De Um Campus Universitário

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