Bacteria related to Bradyrhizobium yuanmingense from Ghana are effective groundnut micro-symbionts

Osei, Ophelia; Abaidoo, Robert C.; Ahiabor, B. D. K.; Boddey, Robert M.; Rouws, Luc Felicianus Marie

doi:10.1016/j.apsoil.2018.03.003

Cited by 25 publications

(18 citation statements)

References 64 publications

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“…This was generally the case for soils in our study locations. Inoculation with native strains in this study led to increases in grain yields of cowpea and groundnut, which confirms the effectiveness of the strains in BNF under controlled conditions previously reported by Osei et al (2018). Furthermore, strains isolated from a particular region have been reported to be the most effective on a given crop in the same region (Sattar et al, 1995).…”

Section: Discussionsupporting

confidence: 89%

“…These varieties were selected based on farmers' preference. The two elite Bradyrhizobium strains (KNUST 1002 and KNUST 1006), selected from preliminary controlled experiments based on their symbiotic effectiveness (Osei et al, 2018), were processed into inoculant formulations under aseptic conditions. Each strain was cultured in 50 ml yeast mannitol broth (YMB) until the late logarithm phase, and this broth was then mixed with 50 g of prepackaged and gamma-radiated (sterile) peat.…”

Section: Preparation Of Planting Materialsmentioning

confidence: 99%

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Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

et al. 2020

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The existence of large population of ineffective native rhizobia and inconsistent performance of exotic strains in Ghanaian soils necessitate the need to identify effective and locally adapted elite strains for enhanced legume-rhizobium symbiosis. This study was designed to test the suitability of two previously selected potential elite Bradyrhizobium strains for use as inoculants on cowpea (Vigna unguiculata L. Walp) and groundnut (Arachis hypogaea L.) in multilocation experiments. Field experiments were set up at 26 locations (12 planted with cowpea and 14 planted with groundnut) in the Northern region of Ghana. Four treatments were applied at each location: inoculation with Bradyrhizobium strains KNUST 1002 and KNUST 1006, a positive nitrogen (+N) control and a negative control (without nitrogen or inoculation) arranged in randomized complete blocks with four replications. The results showed that inoculation with strains KNUST 1002 and KNUST 1006 promoted significant increases in grain yields of both cowpea and groundnut. On average, inoculating cowpea with strains KNUST 1002 and KNUST 1006 resulted in 63 and 52% increases in grain yield when compared to the negative control. Pod yields of groundnut, on the other hand, were significantly (p < 0.05) increased at 57% of the study locations with one or both test strains. Responses to inoculation were, however, highly variable across the different study locations (i.e., significant Treatment × Location Interaction, TLI). A detailed analysis of this significant TLI based on the genotype main effect (G) plus genotype-by-environment (GE) interaction (GGE) biplot analysis revealed that location contributed 71 and 88% of the variation observed in cowpea and groundnut, respectively, and grouped the locations into mega-environments for cowpea. These results demonstrate that native elite Bradyrhizobium strains KNUST 1002 and KNUST 1006 have potential for use as inoculants to increase cowpea and groundnut production in Northern Ghana.

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Section: Discussionsupporting

confidence: 89%

Section: Preparation Of Planting Materialsmentioning

confidence: 99%

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

et al. 2020

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“…The amount of N-fixed by groundnut can vary hugely depending on the genotype (Mokgehle et al, 2014) and the efficacy of the rhizobial strains involved in the symbiosis (Paffetti et al, 1998). In general, however, groundnut is known to have high N 2 -fixing capacity as it can obtain about 88–93% of its N nutrition from BNF and contribute up to 206 kg N ha -1 in cropping systems (Boddey et al, 1990; Peoples et al, 1991; Toomsan et al, 1995; Hoa et al, 2002; Osei et al, 2018). Estimates of N contribution by groundnut was up to 188 kg N ha -1 in South Africa (Mokgehle et al, 2014), 58–101 kg N ha -1 in Ghana (Konlan et al, 2013), and 19–79 kg N ha -1 in Zambia (Nyemba and Dakora, 2010).…”

Section: Groundnut Nodulation By Diverse Rhizobial Populations In Africamentioning

confidence: 99%

Widespread Distribution of Highly Adapted Bradyrhizobium Species Nodulating Diverse Legumes in Africa

Jaiswal

Dakora

2019

Front. Microbiol.

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Bradyrhizobium is one of the most cosmopolitan and diverse bacterial group nodulating a variety of host legumes in Africa, however, the diversity and distribution of bradyrhizobial symbionts nodulating indigenous African legumes are not well understood, though needed for increased food legume production. In this review, we have shown that many African food legumes are nodulated by bradyrhizobia, with greater diversity in Southern Africa compared to other parts of Africa. From a few studies done in Africa, the known bradyrhizobia (i.e., Bradyrhizobium elkanii, B. yuanmingense ) along with many novel Bradyrhizobium species are the most dominant in African soils. This could be attributed to the unique edapho-climatic conditions of the contrasting environments in the continent. More studies are needed to identify the many novel bradyrhizobia resident in African soils in order to better understand the biogeography of bradyrhizobia and their potential for inoculant production.

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“…Los genes nifH se encuentran diversificados y su transcripción está controlada por la ARN polimerasa, que es una holoenzima alternativa, y también necesita una proteína activadora nifA (Satyanarayana, Krishna, Pavan, & Jeereddy, 2018). Otros genes identificados por PCR son 16S rRNA (Akemi et al, 2017;Jaiswal, Msimbira, & Dakora, 2017;Tahon & Willems, 2017), porque permite una clasificación filogenética más detallada a nivel de especie (Osei, Abaidoo, Ahiabor, & Boddey, 2018;Ribeiro et al, 2018), genes rec A, atpD, gln II y nod C con análisis de secuencia multilocus (MLSA) (Leite et al, 2017;Mwenda, Hara, De Meyer, Howieson, & Terpolilli, 2018;Rigonato et al, 2017). Con esta técnica, se ha logrado identificar el gen nifH en géneros Paenibacillus, Enterobacter, Klebsiella y Pantoea (Ndungu et al, 2018), que no son reconocidos por fijar nitrógeno, de modo que es posible que hayan obtenido la capacidad de fijación de nitrógeno a través de la transferencia lateral de genes; sin embargo, las fuerzas evolutivas de la transferencia lateral de genes no son bien conocidas (Khalifa & AlMalki, 2018).…”

Section: Técnicas Pcr Identificación De Los Genes Nifhunclassified

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

Fonseca-López

Quila

Balaguera-López

2019

CTA

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La economía del nitrógeno es un tema que viene creciendo a nivel mundial, especialmente en la actividad agrícola cuando por mal manejo de la fertilización ocurren pérdidas por lixiviación que contribuyen a la eutrofización del agua, lo que incrementa la población de algas y, como efecto, reduce la disponibilidad de oxígeno, acelerando el proceso de desnitrificación en el que se produce N2O, conocido por su efecto en el calentamiento global. Igualmente, parte del fertilizante es emitido a la atmósfera por volatización. Esto ha impulsado la creación de técnicas que permiten cuantificar el nitrógeno utilizado por las plantas y el fijado en el suelo a través de microrganismos para hacer más eficiente el empleo del nitrógeno en los sistemas agrícolas. Sin embargo, dentro de las mayores limitantes se encuentra su sensibilidad, especificidad, costo y tecnología que se requiere para aplicarlas. Esto ha conducido a la innovación de procedimientos y a la creación de técnicas que tienen una tasa de error muy bajo. El objetivo de este trabajo fue realizar la descripción de las principales técnicas utilizadas para cuantificar la fijación del nitrógeno con énfasis en los antecedentes, procedimientos, expresiones matemáticas que se usan, y escenarios futuros. La información se describe a partir del análisis de ensayos disponibles en la base de datos Scopus. Este trabajo consolida las técnicas que continúan vigentes para cuantificar el nitrógeno y facilita entender su uso a través del tiempo con modelos de predicción, así como su importancia, ventajas y desventajas.

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Bacteria related to Bradyrhizobium yuanmingense from Ghana are effective groundnut micro-symbionts

Cited by 25 publications

References 64 publications

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

Native Bradyrhizobium Strains From Ghana Can Enhance Grain Yields of Field-Grown Cowpea and Groundnut

Widespread Distribution of Highly Adapted Bradyrhizobium Species Nodulating Diverse Legumes in Africa

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

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