Azithromycin in the prophylaxis of opportunistic infections in AIDS

McCutchan, J. Allen

doi:10.1258/0956462961917203

Cited by 3 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most important members of this class is the azithromycin (Figure 1), which has been used as antibiotic in the human medicine for the treatment or prophylaxis of a number of health problems such as ear, lung, skin, and throat infections, 3,4 veneral disease, 5 pneumonia, 6 toxoplasmosis 7 and opportunistic diseases in patients with AIDS. 8 Several analytical methods can be found in the literature for the azithromycin determination in different kinds of samples. Undoubtedly, high performance liquid chromatography (HPLC) with different detectors is the Vol.…”

Section: Introductionmentioning

confidence: 99%

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Paula¹,

Almeida²,

Cassella³

2010

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Neste trabalho é proposto um novo método para a determinação espectrofotométrica simples e rápida de azitromicina em formulações farmacêuticas. O método está baseado na reação de transferência de carga entre a azitromicina e a quinalizarina em meio de metanol. O efeito de diversas variáveis foi avaliado com o intuito de alcançar máxima sensibilidade analítica. A reação de transferência de carga foi caracterizada, sendo determinadas a estequiometria da reação, a absortividade molar aparente e a constante de associação. Melhores resultados foram obtidos em meio de metanol e com uma concentração de quinalizarina de 50 mg L -1 . Nestas condições, o ânion radicalar (produto da reação de transferência de carga) foi formado imediatamente após a mistura dos reagentes, apresentando máxima absorção em 564 nm. O método apresentou um limite de detecção de 0,35 mg L -1 e um limite de quantificação de 1,2 mg L -1 , sendo aplicado com sucesso na determinação de azitromicina em três amostras de medicamentos comerciais contendo azitromicina. This paper proposes a new method for simple and fast spectrophotometric determination of azithromycin in pharmaceutical formulations. The method is based on the charge transfer reaction between the azithromycin and quinalizarin in methanol medium. In order to achieve maximum sensitivity the effect of some chemical variables such as the type of solvent, reagent concentration and reaction time were evaluated. The reaction was characterized in terms of stability of the product formed and its stoichiometry, and the apparent molar absorptivity and association constant were derived. Best conditions for the analytical determination of azithromycin were observed in methanol medium with a quinalizarin concentration of 50 mg L -1 . At these conditions, the radical anion (absorbing specie) was formed in the medium immediately after mixing of the reagents and showed maximum absorption at 564 nm. The method presented a limit of detection of 0.35 mg L -1 and a limit of quantification of 1.2 mg L -1 . It was successfully applied in the determination of azithromycin in three commercial pharmaceutical formulations of azithromycin and no matrix interferences were observed.

show abstract