Avaliação macro e microscópica da cicatrização de lesões experimentalmente provocadas em pele de coelhos tratadas com secreção mucoglicoproteica do escargot Achatina fulica

Martins, Maria de Fátima; Caetano, Flavia Aparecida Macedo; Sírio, Otávio José; Yiomasa, Mamie Mizusaki; Mizusaki, Cristina Ioshie; Figueiredo, Larissa Deadame de; Pacheco, Pedro

doi:10.1590/s1413-95962003000900009

Cited by 8 publications

(11 citation statements)

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“…In this study, the mucous secretion of A. fulica inhibited the bacterial growth of both have been previously reported (Iguchi et al;Kubota;Martins et al;Sirio et al;Lorenzi & Martins, 2006). It has been proposed that this mucous secretion is rich in lysozyme, opsonines, and other antibacterial and antiviral factors (Fuchino & Barrett, 1992).…”

Section: Morphological Analysis Of the Histological Sections Stained supporting

confidence: 75%

“…To determine the minimal fungicidal concentration were used tubes containing 5.0 mL of BHI broth, 4 mL of mucus in each group, followed by serial dilution concentrations producing between 290 and 14,800 mg.mL-1 of mucus (Martins et al, 2003). To the tubes we added 1 mL of each inoculum of 0.5 McFarland (1.5 x 108 CFU) and incubated for 37 º C/24 hours (Cruse & Foord, 1980;Moylan & Kennedy, 1980).…”

Section: Methodsmentioning

confidence: 99%

“…Previous investigations attesting the healing potential of the mucus of Achatina fulica have been reported in this decade (Caetano, 2000;Yomassa, 2002;Martins et al;Sirio et al), but this is the first one to process this biological material as films for wound dressing. In this study, neither infiltration of eosinophils, a typical morphological sign of allergenic reaction (Chowdary et al, 2003), nor clusters of epithelioid and foreign body giant cells, classical feature of biological incompatibility (Maluf-Meiken et al, 2006) were observed within the wounds, attesting the good acceptability of the films by dermal tissues of the animals.…”

Section: Morphological Analysis Of the Histological Sections Stained mentioning

confidence: 98%

“…Achatina fulica is a land snail able to produce a glycoproteic secretion which has been demonstrated to present some biological effects, such as antibacterial properties against gram positive and negative microorganisms (Iguchi et al, 1982;Kubota, 1985;Martins et al, 2003;Sirio et al, 2005, Lorenzi & Martins, 2006. Furthermore, mucus of this snail has been tested in surgical wounds of experimental animals and proved to improve the dermal cicatricial repair (Martins et al; Sirio et al).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Antimicrobial Activity and Healing Potential of Mucous Secretion of Achatina fulica

et al. 2012

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SUMMARY: Achatina fulica's secretion has been recently related to antibacterial, antifungal, and cicatricial properties, and it is influenced by the kind of food offered. Therefore, this study investigated the healing potential of dressing films based on mucous secretion of Achatina fulica. Thus, surgical wounds performed in black wistar rats were dressed with films based on collagen, and on mucous secretion of A. fulica fed with Lactuca sativa; undresses worked as wounds control. After 3, 7, 14 and 21 days the animals were euthanized, and the wounds were microscopically evaluated. On the 3rd and 7th days dressing films based on mucus provided acceleration of the formation maturation of granulation tissue, better epithelization rates, and more rapid replacement of type III for type I collagen fibers. On the 14th and 21st days, these dressings induced more intense deposition and better architectural disposition of type I collagen fibers, and hastened the regeneration of cutaneous phaneros. Dressings obtained from A. fulica fed with Lactuca sativa provided the most expressive results. This study suggests that films produced with mucous secretion of A. fulica can be successfully employed as wound dressing, particularly if snails are fed with Lactuca sativa.

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Section: Morphological Analysis Of the Histological Sections Stained supporting

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Morphological Analysis Of the Histological Sections Stained mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Assessment of Antimicrobial Activity and Healing Potential of Mucous Secretion of Achatina fulica

et al. 2012

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“…Thus, QC and BC stimulated an early and strong fibroblast proliferation, compared to other treatments, where the proliferation occurred much later and for a longer period. Martins et al (2003) also mentioned that the barbatiman favored the fibroplasia process in the wounds of horses, referring this action to the low oxygen tension and the accumulation of fibrin provided by the phytotherapeutic in the center of the lesion. The immunomodulatory properties of chitosan were confirmed; fibroblast proliferation was marked with this treatment, similar to the results presented by Silva et al (2006).…”

Section: Periodsmentioning

confidence: 99%

Barbatiman and chitosan creams as adjuvants in rabbit skin wound healing

Lima

Silva

Rabelo

et al. 2016

SCA

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In this study, 5% barbatiman and 5% chitosan creams were evaluated as adjuvants in the tissue repair process by secondary intention of rabbit's skin wounds. Four equidistant wounds were induced in the dorsal skin of 20 adult male rabbits, which were submitted to healing by secondary intention and treated with 5% chitosan cream (QC, n=5), 5% barbatiman cream (BC, n=5), 2% allantoin cream (n=5), and base cream (n=5). The creams were applied with the aid of disposable spatulas after washing the wounds. The wounds were daily analyzed by clinical examination for 21 days and histological analyses were performed on the 3 rd , 14 th , and 21 st day after induction. The microscopic evaluation of the wounds of all groups showed macroscopic features of the healing process at different time intervals. The QC and BC treatments helped in the skin repair process in rabbits when compared to the other two treatments. They induced fibroblast activation and early collagen deposition, and modulated re-epithelialization and neovascularization. Thus, it was concluded that BC and QC are efficient and economically feasible as adjuvants in the healing process of skin wounds in rabbits. Key words: Chitin. Lagomorphs. Phytotherapy. Tissue repair. ResumoNeste estudo, os cremes de barbatimão a 5% e quitosana a 5% foram avaliadas como adjuvantes no processo de reparação tecidual por segunda intenção de lesões cutâneas de coelho. Quatro feridas cutâneas equidistantes foram induzidas na região dorsal de 20 coelhos machos adultos, que foram submetidos a cicatrização por segunda intenção e tratados com creme de quitosana a 5% (QC, n = 5), creme de barbatimão a 5% (BC, n = 5), creme de alantoína a 2% (CA, n = 5), e base de creme (NC; n = 5). A aplicação de creme foi realizada com o auxílio de espátulas descartáveis e, antes deste, cada ferida foi lavada. As feridas foram analisadas por meio de exames clínicos diários durante 21 1 Profª Adjunta, Universidade Estadual de Goiás, UEG, Campus Jataí, Jataí, GO, Brasil. E-mail: carolrochavet@hotmail.com 2 Prof. Titular, Escola de Medicina Veterinária e Zootecnia, UFG, Regional Goiânia, Goiânia, GO, Brasil. E-mail: prof_ufg.dmv@ hotmail.com 3 Profs. Ajunto do Curso de Medicina Veterinária, UFG, Regional Jataí, Jataí, GO, Brasil. E-mail: rabelovet@yahoo.com.br; aloisiosv@hotmil.com 4 Discente do Curso de Doutorado do Programa de Pós-Graduação em Ciência Animal, UFG, Regional Goiânia, Goiânia, GO,Brasil. E-mail: bruno.moraes.assis@gmail.com 5 Prof. Ajunto, Instituto de Patologia Tropical e Saúde Pública, IPTSP, UFG, Regional Goiânia, Goiânia, GO, Brasil. E-mail:wdzlopes@hotmail.com 6 Discentes do Curso de Medicina Veterinária, UFG, Regional Jataí, Jataí, GO, Brasil. E-mail: lucas.mascarenhas@hotmail.com. br; rhavilla_@hotmail.com * Author for correspondence dias corridos e por análises histológicas no 3º, 14ª e 21º dias após a indução. As feridas de todos os grupos apresentaram características macroscópicas correspondentes com os observados no processo de cicatrização na avaliação microscópica nos ...

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Caracterización físico-química y actividad antimicrobiana de la secreción mucosa de Achatina fulica

Pereira¹,

P²,

R³

et al. 2016

revsal

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Avaliação macro e microscópica da cicatrização de lesões experimentalmente provocadas em pele de coelhos tratadas com secreção mucoglicoproteica do escargot Achatina fulica

Cited by 8 publications

References 0 publications

Assessment of Antimicrobial Activity and Healing Potential of Mucous Secretion of Achatina fulica

Assessment of Antimicrobial Activity and Healing Potential of Mucous Secretion of Achatina fulica

Barbatiman and chitosan creams as adjuvants in rabbit skin wound healing

Caracterización físico-química y actividad antimicrobiana de la secreción mucosa de Achatina fulica

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