Caracterización físico-química y actividad antimicrobiana de la secreción mucosa de Achatina fulica

Pereira, Andrés Esteban; P, Arley Rey; R, Jenny P López; O, Jennifer P Castro; D, Nelson Uribe

doi:10.18273/revsal.v48n2-2016003

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…Even though there are studies that demonstrate the antimicrobial properties of the mucus secretion of A. fulica, little is known about the activity oofresistant bacteria. Our previous studies confirmed the inhibitory action of mucoprotein and protein fractions of mucus secretion against S. aureus CMPUJ 015 (Pereira et al, 2016;Suárez et al, 2021). In this study, new analogs of haemocyaninderived peptides identified in >30kDa protein fractions from mucus secretion of A. fulica, were also active against S. aureus CMPUJ 015, S. aureus ATCC 29213, S. aureus ATCC 43300 and S. aureus ATCC 25922.…”

Section: Peptide Id Sequencesupporting

confidence: 87%

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Achatina fulicaHaemocyanin-Derived Peptides as Novel Antimicrobial Agents

Pereira,

Suarez,

Roman

et al. 2023

Preprint

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Anteriorly we found Haemocyanin-derived peptides in semi-purified fractions of mucus secretion ofAchatina fulicathat showed an inhibitory effect onS. aureusstrains. Here, we appliedin silicorational design strategy to obtain new potential Antimicrobial Peptides (AMPs) fromA. fulicahaemocyanin-derived peptides (AfH). Designed peptides were chemically synthetized using the Fmoc strategy and antimicrobial activity againstE. coliandS. aureusstrains was investigated using the broth microdilution method. Cytotoxic activity on Vero, HaCat, and human erythrocytes cells was also evaluated. The results showed that 15-residue synthetic peptides, alpha-helical and cationic, were those with the highest biological activity against Gram positive strains, with Minimum Inhibitory Concentrations (MIC) in the range of 20 to 30 µM. The positive selectivity index suggests a higher selectivity mainly on the microorganisms evaluated and not on eukaryotic cells. In this study,A. fulicahemocyanin turned out to be an appropriate protein model for the rational design of AMPs against bacteria of public health importance. Additional studies are required to evaluate the activity of the peptides on Gram negative bacteria.

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Section: Peptide Id Sequencesupporting

confidence: 87%

“…The specimens of A. fulica identified by morphological characteristics were collected in Floridablanca, Santander, Colombia. Mucus secretion was obtained by direct stimulation on the foot with 9V electric current at intervals of 30 to 60 seconds, according to the protocol stablished in (Pereira et al, 2016).…”

Section: Mucus Collectionmentioning

confidence: 99%

Achatina fulicaHaemocyanin-Derived Peptides as Novel Antimicrobial Agents

Pereira,

Suarez,

Roman

et al. 2023

Preprint

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“…Glycosaminoglycan and 6‐gingerol endowed WJSS with wound‐healing and anti‐apoptosis properties 29 . Hemocyanin and mucin fractions have antimicrobial activity 30,31 . Except for the weight loss of each group on the following day due to 24 h fasting on the last day of the experiment, there was no adverse effect of WJSS on mice in terms of body weight, which indicates that WJSS has no apparent toxic side effects.…”

Section: Discussionmentioning

confidence: 93%

Effect of white jade snail secretion on antioxidant capacity and intestinal microbial diversity in mouse model of acute gastric ulcer

Cheng,

Wang,

et al. 2023

J Sci Food Agric

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BackgroundIn the present work, acute gastric ulcer models were constructed by administering hydrochloric acid/ethanol. The mice ingested WJSS through gastric infusion. Ulcer areas in gastric tissue were recorded, and malondialdehyde (MDA) and superoxide dismutase (SOD) were also measured. Notably, high‐throughput 16s rDNA analysis of intestinal flora and determination of amino acid composition in feces were performed to understand the effect of WJSS on model mice.ResultsCompared with the control group, the ulcer area in the WJSS low, middle and high‐concentration groups declined by 28.02%, 39.57% and 77.85%. The MDA content decreased by 24.71%, 49.58% and 64.25%, and the SOD relative enzyme activity fell by 28.19%, 43.37% and 9.60%. The amounts of amino acids in the low, middle and high‐concentration groups were slightly lower, and probiotic bacteria such as Bacteroidetes and Lactobacillales increased in different concentration WJSS groups. Adding WJSS contributes to the establishment of beneficial intestinal flora and the absorption of amino acids.ConclusionOur results showed that WJSS has a good effect on inhibiting the hydrochloric acid‐ethanolic gastric ulcer, suggesting that WJSS has excellent potential as a novel anti‐ulcer agent. Combined with ulcer area, MDA content, SOD content, gut probiotics and other indicators, a high concentration of WJSS had the best protective effect on acute gastric.This article is protected by copyright. All rights reserved.

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“…Achacin from African giant snail mucus inhibited the growth of Escherichia coli and Staphylococcus aures spp [26]., while the MIC 50 value resulted effective against Streptococcus agalactiae and Methicillin-Resistant Staphylococcus aureus (MRSA) [37], as reported in Table 1.…”

Section: Antimicrobial Activitymentioning

confidence: 97%

“…Achacin [23,26,37], Achatin CRP (C-reactive protein) [38] and Mytimacin-AF [39] and Hemocyanin β c-HaH [40] are antimicrobial peptide isolated from Achatina fulica and Helix aspersa mucus, respectively.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial properties of terrestrial snail and slug mucus

Cilia

Fratini

2018

Journal of Complementary and Integrative Medicine

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Snail and slug mucus is a viscous-elastic substance secreted by specific glands with adhesive and lubricants properties that allows them to adhere tenaciously to many different surfaces. It has been used since ancient times for care and human health and it is still very important in traditional and folkloristic medicine. Recently, mucus from snail and slugs and its protein and components have been subjected to some investigations on their antibacterial, antiviral and antifungal activity due to extensive traditional uses and for a future application in medicine. Antimicrobial activities of crude mucus, and its components, against different microorganism have been reported, showing antimicrobial activities that lead their potential employment in several fields as natural additives. The purpose of this Review is to summarize the results of antimicrobial studies of snail and slug mucus and its compounds from the first scientific applications to the isolation of the single components in order to better understand its application and propose an employment in future studies as a natural antimicrobial agent.

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Caracterización físico-química y actividad antimicrobiana de la secreción mucosa de Achatina fulica

Cited by 4 publications

References 13 publications

Achatina fulicaHaemocyanin-Derived Peptides as Novel Antimicrobial Agents

Achatina fulicaHaemocyanin-Derived Peptides as Novel Antimicrobial Agents

Effect of white jade snail secretion on antioxidant capacity and intestinal microbial diversity in mouse model of acute gastric ulcer

Antimicrobial properties of terrestrial snail and slug mucus

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