Avaliação da deposição de colágeno após implante de fáscia lata e de gordura na prega vocal de coelho: estudo histomorfométrico

Abstract

Several materials have been injected or introduced in the vocal folds in attempt of solving the glottic insufficiency. However, few studies have evaluated the cicatricial process due to the implantation of these materials. Aim: The objective of this research was to evaluate the concentration of collagen after microsurgery graft of muscular fascia and fat in the vocal folds of rabbit. Study design: experimental. Material and Method: Nineteen rabbits were submitted to the graft insert in the right vocal fold, being nine of fascia and ten of fat. The left vocal fold was submitted to the same process, except for the insertion of fat or fascial graft. The rabbits were sacrificed after 90 and 180 days. The collagen was analyzed through the method of the Picrosirius-polarization using the Image Pro Plus software. Results: There was prevalence of the collagen in all grafted groups when compared with the group control. The concentration of the collagen found in the rabbits submitted to fat graft was significantly larger when compared to the concentration of the rabbits submitted to graft of muscular fascia, either with 90 as with 180 days. Conclusion: The fat and muscular fascia implantation in the vocal folds of rabbit promoted production of collagen, being more intense with fat.

Collagen‐Based Films Containing Liposome‐Loaded Usnic Acid as Dressing for Dermal Burn Healing

“…Nevertheless, UAL showed a mix of wavy and highly interlaced type I and type III collagen fibers, whose arrangement resembled the normal dermis (Figure 4(IIIc)). The replacement of a substantial part of the content of type III collagen for type I molecules during the healing process is an absolutely expected phenomenon [35, 36]. However, the excessive production of type I collagen, as observed in COL and PHO, might easily lead to the formation of undesirable hypertrophic scars and keloids [37, 38].…”

Effect of Laser Therapy in Inflamed Tissue by Medications Based on Iodoform Laser Therapy in Inflamed Tissue

“…The type III collagen form thin and delicate fibers, loosely arranged showing a weak birefringence of greenish and yellowish coloration -while type I collagen form fibers of variable thickness, arranged parallel or interlaced, with yellow-orangish and reddish bi-refringence (Dantas et al, 2011;Carneiro et al, 2005). During the formation of the cicatricial process, collagen type III is initially synthesized and deposited and acts as a framework for migration of endothelial cells that will form the granulation reaction, being replaced by type I occurs as it cicatricial tissue maturation (Carneiro et al, 2005;Fleischmajer et al, 1990). This process has relevance for the dermal resistance in cicatricial area, since the increase in the relation between collagen type I and type III is essential for the tensile strength and mechanical stability of connective tissue (Friedman et al, 1993).…”

“…The histological cuts stained with Pricosiris Red and analyzed under polarized light were used for qualitative analysis of the collagen fibers deposition (Dantas et al, 2011;Carneiro et al, 2005).The collagen fibers were analyzed according to bi-refraction pattern phenomenon for characterization of the type of collagen fiber (greenish/greenish yellow-fiber type-III or orangish/reddish-orange -type I fibers), the morphological appearance (short or long, thin or thick) and arrangement of collagen fibers (reticular, parallel or interlaced).…”

Estudo histológico do tecido conjuntivo areolar perifascial implantado em pregas vocais de coelhos

“…Embora não apresente uma estruturação trilaminar como na prega vocal humana (Hirano et al, 1974), a lâmina própria da prega vocal de coelhos pode ser dividida em duas camadas (Kurita et al, 1995). Outros aspectos que levaram à escolha desse animal é a existência de vários estudos que utilizaram o coelho como modelo experimental para avaliação do processo cicatricial em pregas vocais e resposta ao implante de tecidos autólogos, nos fornecendo parâmetros comparativos , Rousseau et al, 2004Carneiro et al, 2005;Murta et al, 2005) A escolha dos períodos pós-enxertia em que os animais foram sacrificados foi baseada em análise de estudos prévios.…”

“…A gordura apresenta viscoelasticidade semelhante à da prega vocal, é um material biocompatível, e é considerado um excelente substituto para o espaço de Reinke (Chan e Titze, 1998;. Entretanto, seu uso tem sido desencorajado por estudos que mostram sua rápida absorção e promoção de fibrose (Mikus et al, 1995;Carneiro et al, 2005). (1987) Kriesel et al, 2002).…”