Avaliação da atividade antibacteriana de extratos de plantas medicinais: Significância sanitária em região de tríplice fronteira

Abstract

In this thesis, the objective was to evaluate the in vitro antibacterial action of extracts of medicinal plants most used in the municipality of Foz do Iguaçu and region, on Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The most used plants in the municipality of Foz do Iguaçu and region, which are: Mikania glomerata (Guaco), Mentha piperita (Hortelã pimenta), Maytenus officinalis (Espinheira-santa) and Aloe vera (Babosa) were prepared as phytotherapics and yielded by Horto de plants of the Itaipu Binacional hydroelectric plant. For this evaluation 70% hydroalcoholic extracts were prepared by percolation and aqueous extracts by infusion of these plants. The hydroalcoholic extracts were rotoevaporated and the aqueous extracts were added to the lyophilizer. The peppermint essential oil was also extracted using the water vapor distillation method in a Clevenger apparatus followed by thin layer chromatography. The antibacterial activity of the extracts was tested on the standard strains of Pseudomonas aeruginosa ATCC (27853), Staphylococcus aureus ATCC (25923) and Klebsiella pneumoniae ATCC (13883). The determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (CBM) of the plant extracts on the bacterial strains were determined by the microplate microdilution method according to the methodology recommended by the Clinical and Laboratory Standards Institute (CLSI). Among the hydroalcoholic and aqueous extracts of the plants tested, only the hydroalcoholic extract of Guaco (Mikania glomerata) showed positive antibacterial activity on Staphylococcus aureus ATCC 25923, in the concentrations of 400 Wg and 200 Wg. The other hydroalcoholic and aqueous extracts presented bacteriostatic activity. Antimicrobial activity of peppermint (Mentha piperita) essential oil on

Produção De Biofilme E Atividade Antibacteriana Do Óleo Essencial De Mentha Piperita Contra Staphylococcus Aureus Isolado De Pacientes Com Rinossinusite Crônica

“…Nonato [10] concluded in his research that from the concentration of 8 µl/mL of essential oil of M. piperita, there is no visible microbial growth of the bacteria S. aureus, according to him, the essential oil of M. piperita at low concentrations it already has a bactericidal effect. Ferreira [11] also concluded in his research that both the essential oil of M. piperita and the hydroalcoholic extract of M. piperita have in vitro antibacterial activity for the bacterial strain of S. aureus.…”

Evaluation of the functional properties of the protein extract obtained from chickpea flour (Cicer arietnum L.)

“…The antimicrobial activity was performed according to the methodology described by Ferreira, 2018. Pure bacterial cultures maintained on agar and stored in a freezer were transferred to Beef Extract (BE) broth, including Escherichia coli CBAM 0001, Salmonella typhi CBAM 0015, and Staphylococcus aureus ATCC 23235, obtained from the FIOCRUZ collection.…”

“…Additionally, its low glycemic index helps control blood sugar levels. Contributing to a healthy and balanced diet for millions of people, it has increased the demand for the grain (Ferreira;Brazaca;Arthur;2006;Kaur;Singh, 2007;Chavan et al, 2009;Jukanti et al, 2012).…”

Actividad antibacteriana in vitro de Maytenus ilicifolia Martius sobre Streptococcus mutans y Staphylococcus aureus

“…quinonametídeos (friedelanol y friedelina); flavonoides; terpenos (maitenina, tringenona, isotenginona II, congorosinas A y B, ácido maitenóico); aceites escenciales (friedenelo)l; alcaloides (maitenina, maitanprina y maitensina); taninos, principalmente los gálicos (epicatequina, epigalocatequina y galato de epigalocatequina); glicolípidos (monogalactosildiacilglicerol, digalactosildiacilglicerol, trigalactosildiacilglicerol, tetragalactosildiacilglicerol y sulfaquinovosildiacilglicerol. (Ferreira H, 2019).…”