Available lysine. II.—Determination of available lysine in feedingstuffs by dinitrophenylation

Matheson, N. A.

doi:10.1002/jsfa.2740190904

Cited by 17 publications

(4 citation statements)

References 22 publications

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“…The widely used method described by Carpenter (1960) involves 1-fluoro-2,4-dinitrobenzene (FDNB) to tag lysine residues and then measurement of N-dinitrophenyled lysine (DNP-lysine) spectrophotometrically after acid hydrolysis. The presence of carbohydrates in the sample may lead to formation of interfering compounds and therefore cause the amount of reactive lysine to be overestimated (Matheson, 1968). Attempts have been made to use chromatography to separate DNP-lysine from interfering amino acids and from other compounds formed during hydrolysis of carbohydrates contained in foods (Holm, 1971).…”

Section: Introductionmentioning

confidence: 99%

HPLC Determination of the Reactive Lysine Content of Cottonseed Protein Films To Monitor the Extent of Cross-Linking by Formaldehyde, Glutaraldehyde, and Glyoxal

Marquié

Tessier

Aymard

et al. 1997

J. Agric. Food Chem.

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A sensitive and reproductible HPLC method to determine the reactive lysine content of films made from cottonseed flour was developed. This method is applied to follow the cross-linking of cottonseed proteins by formaldehyde, glutaraldehyde, or glyoxal. The cross-linking of these proteins resulted in the decrease of reactive lysine content within the film. The maximum puncture force of the films is correlated with the modified reactive lysine content after cross-linking treatments. Of the three cross-linking agents, formaldehyde was the most effective to enhance the maximum puncture force of the films despite its moderate reaction with only 50% of reactive lysine in the films. By contrast, glutaraldehyde, which reacted with nearly 100% of lysine, led to less resistant films. The results were interpreted as the impact of the molecular structure of the cross-link bridges on the mobility between protein chains. Keywords: Cross-linking; HPLC; cottonseed proteins; films; reactive lysine

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Section: Introductionmentioning

confidence: 99%

HPLC Determination of the Reactive Lysine Content of Cottonseed Protein Films To Monitor the Extent of Cross-Linking by Formaldehyde, Glutaraldehyde, and Glyoxal

Marquié

Tessier

Aymard

et al. 1997

J. Agric. Food Chem.

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“…Some agricultural or ecological journals direct authors and reviewers to some of the relevant reporting guidelines (e.g. the International Journal of Ecology (Published by Hindawi) [23], Agricultural Research (Springer) [24], and the Journal of the Science of Food and Agriculture (Wiley) [25], but there is no consolidated repository where users can easily find the relevant guidelines (e.g. the Equator Network for the medical sciences).…”

Section: Introductionmentioning

confidence: 99%

AgroEcoList 1.0: A checklist to improve reporting standards in ecological research in agriculture

Daykin,

Aizen,

Barrett

et al. 2023

PLoS ONE

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Many publications lack sufficient background information (e.g. location) to be interpreted, replicated, or reused for synthesis. This impedes scientific progress and the application of science to practice. Reporting guidelines (e.g. checklists) improve reporting standards. They have been widely taken up in the medical sciences, but not in ecological and agricultural research. Here, we use a community-centred approach to develop a reporting checklist (AgroEcoList 1.0) through surveys and workshops with 23 experts and the wider agroecological community. To put AgroEcoList in context, we also assessed the agroecological community’s perception of reporting standards in agroecology. A total of 345 researchers, reviewers, and editors, responded to our survey. Although only 32% of respondents had prior knowledge of reporting guidelines, 76% of those that had said guidelines improved reporting standards. Overall, respondents agreed on the need of AgroEcolist 1.0; only 24% of respondents had used reporting guidelines before, but 78% indicated they would use AgroEcoList 1.0. We updated AgroecoList 1.0 based on respondents’ feedback and user-testing. AgroecoList 1.0 consists of 42 variables in seven groups: experimental/sampling set-up, study site, soil, livestock management, crop and grassland management, outputs, and finances. It is presented here, and is also available on github (https://github.com/AgroecoList/Agroecolist). AgroEcoList 1.0 can serve as a guide for authors, reviewers, and editors to improve reporting standards in agricultural ecology. Our community-centred approach is a replicable method that could be adapted to develop reporting checklists in other fields. Reporting guidelines such as AgroEcoList can improve reporting standards and therefore the application of research to practice, and we recommend that they are adopted more widely in agriculture and ecology.

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“…In the FDNB method modifications to overcome loss of DNP lysine during hydrolysis and to remove interference have been tried by many workers with limited success. [11][12][13][14][15][16][17][18] Interference by carbohydrate in the TNBS method has been attributed to caramelisation with the subsequent production of non-ether extractable sugars and to adsorption of TNP lysine on to fine carbon particles.' Methods for overcoming these effects have included modifying the "Current address: Department of Chemistry, University of Guyana, Georgetown, Guyana.…”

Section: Introductionmentioning

confidence: 99%

The rapid determination of chemically reactive lysine in the presence of carbohydrates by a modified trinitrobenzenesulphonic acid procedure

James

Ryley

1986

J Sci Food Agric

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The trinitrobenzene sulphonic acid (TNBS) method has been shown by previous workers to give low results for chemically reactive lysine if carbohydrates are present in the sample. The major factor responsible has now been identified as loss of E-trinitrophenyl (E-TNP) lysine during the hydrolysis stage, while adsorption of E-TNP lysine by humins formed from carbohydrates during hydrolysis has been shown to be insignificant. The loss can be minimised by a reduction of the hydrolysis time without affecting the necessary degree of protein hydrolysis. A modified procedure is suggested which is shown to give very similar results to Carpenter's method (incorporating the Booth modification) for pure proteins and to the Silcock method in the presence of carbohydrates. E-TNP lysine hydrolysed for 90 min or D,L-lysine taken through the trinitrophenylation procedure followed by hydrolysis for 120 min are the most suitable standards. The procedure can be completed in under 6 h and does not require the use of expensive equipment or hazardous chemicals.

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Available lysine. II.—Determination of available lysine in feedingstuffs by dinitrophenylation

Cited by 17 publications

References 22 publications

HPLC Determination of the Reactive Lysine Content of Cottonseed Protein Films To Monitor the Extent of Cross-Linking by Formaldehyde, Glutaraldehyde, and Glyoxal

HPLC Determination of the Reactive Lysine Content of Cottonseed Protein Films To Monitor the Extent of Cross-Linking by Formaldehyde, Glutaraldehyde, and Glyoxal

AgroEcoList 1.0: A checklist to improve reporting standards in ecological research in agriculture

The rapid determination of chemically reactive lysine in the presence of carbohydrates by a modified trinitrobenzenesulphonic acid procedure

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