Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4

Li, X.; Eck, Herman J. van; Voort, J.N.A.M. Rouppe van der; Huigen, D. J.; Stam, P.; Jacobsen, E.

doi:10.1007/s001220050847

Cited by 115 publications

(63 citation statements)

References 21 publications

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“…For the selective amplification, 11 E+3/M+3 primer combinations were selected based on their appearance in the Ultra High Density (UHD) Map (http://www.dpw.wau.nl/uhd/). Extra primer combinations were selected according to the results of a bulked segregant analysis (BSA) with 204 primer combinations as described by Michelmore et al (1991) and six primer combinations from the R2 map (Li et al 1998).…”

Section: Dna Isolation and Aflp Analysismentioning

confidence: 99%

“…In addition to this, AFLP marker analysis was performed to detect the wild species origin of the resistance in five different Solanum species as described by Li et al (1998). The mobility of the AFLP markers, which are linked to the resistance locus, was compared.…”

Section: Dna Isolation and Aflp Analysismentioning

confidence: 99%

“…In the UHD mapping population, Th2 was localized on chromosome 4. R2, one of the S. demissum-derived R genes against P. infestans, was also mapped on chromosome 4 (Li et al 1998). To confirm the chromosome number, six primer combinations selected from the R2 map were tested on our mapping population of 233 genotypes and four R2-linked markers were grouped with the Rpi-abpt locus in the third round of JoinMap analysis.…”

Section: Localization Of the Resistance Locusmentioning

confidence: 99%

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High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

et al. 2005

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Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to cosegregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster.

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Section: Dna Isolation and Aflp Analysismentioning

confidence: 99%

Section: Dna Isolation and Aflp Analysismentioning

confidence: 99%

Section: Localization Of the Resistance Locusmentioning

confidence: 99%

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High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

et al. 2005

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“…Other R genes originating from S. demissum were mapped to diVerent positions. R2 was localized on chromosome IV (Li et al 1998). Genes R3, R6 and R7 were all mapped to a distal position on chromosome XI (El-Kharbotly et al 1994.…”

Section: Introductionmentioning

confidence: 99%

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

et al. 2006

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Despite the long history of breeding potatoes resistant to Phytophthora infestans, this oomycete is still economically the most important pathogen of potato worldwide. The correlation of high levels of resistance to late blight with a long vegetation period is one of the bottlenecks for progress in breeding resistant cultivars of various maturity types. Solanum phureja was identiWed as a source of eVective late blight resistance, which was transferred to the cultivated gene pool by interspeciWc crosses with dihaploids of Solanum tuberosum. A novel major resistance locus, Rpiphu1, derived most likely from S. phureja and conferring broad-spectrum resistance to late blight, was mapped to potato chromosome IX, 6.4 cM proximal to the marker GP94. Rpi-phu1 was highly eVective in detached leaXet, tuber slice and whole tuber tests during 5 years of quantitative phenotypic assessment. The resistance did not show signiWcant correlation with vegetation period length. Our Wndings provide a well-characterized new source of resistance for breeding early and resistant-to-P. infestans potatoes.

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“…For tagging qualitative traits that can be easily classified into two or more discreet classes, BSA is particularly efficient. The advantage of this approach is that the likelihood of identifying false positive markers is very small (Michelmore et al 1991, Meksem et al 1995, Li et al 1998. The combination of the AFLP technique and BSA has been effectively used to generate molecular markers that are closely linked to the genes of interest in many crops (Chantret et al 2000, Miftahudin et al 2002, Asnaghi et al 2004.…”

Section: Discussionmentioning

confidence: 99%

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

et al. 2009

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As a first step toward cloning the genic multiple allele inherited male-sterile gene Ms in Chinese cabbage (Brassica rapa L. ssp pekinensis (Lour.) Olsson), we attempted the molecular tagging of this male-sterility locus using the amplified fragment length polymorphism (AFLP) technique. A BC 1 mapping population (130 plants) segregating for male sterility/fertility was constructed for the tagging using the bulked segregant analysis (BSA) method. Screening 144 primer combinations, four AFLP markers were identified to be tightly linked to the Ms gene, the nearest one of which, AFLP01, was converted to a SCAR marker for the markerassisted selection (MAS). Finally, a genetic map of the Ms gene was constructed with four AFLP markers and one SCAR marker, covering a total interval of 13.9 cM. Two markers AFLP01(SCAR01) and AFLP04 flanked the Ms locus at distances of 2.3 and 7.8 cM, respectively.

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Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4

Cited by 115 publications

References 21 publications

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

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