High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

Abstract

Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to cosegregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster.

Genetic Positioning of Centromeres Using Half-Tetrad Analysis in a 4x–2xCross Population of Potato

“…The male parent RH89-039-16 can be crossed with tetraploid female parents because of the production of 2n pollen. More commonly, clone RH89-039-16 is crossed with other diploids to generate diploid mapping populations (Rouppe van der Voort et al 1997Voort et al , 1998Voort et al , 2000Park et al 2005), including the population that was used to generate the UHD map in potato (Isidore et al 2003;van Os et al 2006). In the UHD map, the genetic position of .10,000 AFLP markers has been determined (http:/ /potatodbase.dpw.wau.nl/UHDdata.html).…”

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

“…In the case of S. stoloniferum, a late blight resistant clone CGN17605-4 was crossed with the susceptible breeding line RH89-039-16, which produces 2n pollen and is frequently used for mapping research at the Laboratory of Plant breeding at Wageningen University and Research Centre (Rouppe van der Voort et al 1998;Huang et al 2004;Park et al 2005). For the late blight resistance test, detached leaf assays (DLAs) were performed as described by Vleeshouwers et al (1999).…”

“…Examples are S. pinnatisectum (Kuhl et al 2001) and S. bulbocastanum (Naess et al 2000;Song et al 2003; Van der Vossen et al 2003 andPark et al 2005) from Mexico and S. berthaultii (Rauscher et al 2006;Ewing et al 2000), S. microdontum (Sandbrink et al 2000), S. mochiquense (Smilde et al 2005) and S. paucissectum (Villamon et al 2005) from Andean countries. In addition, the cultivated diploid S. phureja has been described to contain valuable resistance (Ghislain et al 2001;Sliwka et al 2006).…”

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

“…The second gene Rpi-blb2 is localized on chromosome VI and has also been cloned , whereas Rpi-blb3 has been mapped to chromosome IV (Park et al 2005a). The latter forms an R gene cluster on chromosome IV, together with R2, Rpi-abpt from S. bulbocastanum and a R2-like gene of unknown origin (Park et al 2005b). The RB gene has been introduced into the cultivated potato gene pool by somatic hybridization and Rpi-abpt by bridge crosses.…”

“…The RB gene has been introduced into the cultivated potato gene pool by somatic hybridization and Rpi-abpt by bridge crosses. Both genes confer resistance to contemporary P. infestans isolates and are being used in breeding programs (Song et al 2003;Park et al 2005b).…”