Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

Peng, Wei; Feng, Hui; Piao, Zhongyun; Li, Chengyu; Liu, Zhiyong; Wang, Yugang; Ji, Ruiqin; Zou, Ting; Ji, Shujuan

doi:10.1270/jsbbs.59.333

Cited by 9 publications

(2 citation statements)

References 38 publications

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“…Double-stranded cDNA was digested using the EcoRI and MseI restriction enzymes. AFLP reactions were performed according to the method of Wei et al (2009) using a total of 256 primer combinations derived from 16 EcoRI + NN and 16 MseI + NNN primers. All amplifications were performed in an iCycler (Bio-Rad, Hercules, CA, USA) using the PCR conditions described by Vos et al (1995).…”

Section: Cdna-aflp Analysismentioning

confidence: 99%

“…Some high quality male sterile lines have been bred successfully. With these male sterile materials, two commercial hybrids have been registered and released in China, and some molecular markers have subsequently been identified Wei et al, 2009;Wang et al, 2010). To make better use of these high-quality male sterile lines, it is crucial to isolate fertility-related genes to help understand the genetic mechanism of male sterility.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

Ji¹,

Song²,

Xin³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the twotype line, AB01. Sixteen high-quality sequences were generated, 11 of which were up-regulated in fertile buds, and five of which were upregulated in sterile buds. Based on BLAST screening and functional annotation, these genes have homology with genes encoding known flower-or bud-specific proteins, metabolism-related proteins and cellstructure proteins. In addition, the full-length cDNA sequences of the actin gene were cloned from the cabbage plants by RACE and used as an internal standard for semi-quantitative reverse transcription-PCR. Expression of three flower-or bud-specific differentially expressed transcript-derived fragments in fertile and sterile buds was examined using RT-PCR; the expression patterns of these genes were similar to the patterns observed in the cDNA-AFLP analysis.

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Section: Cdna-aflp Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

Ji¹,

Song²,

Xin³

et al. 2011

Genet. Mol. Res.

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Amplified Fragment Length Polymorphism: Applications and Recent Developments

Sheeja

Kumar

Giridhari

et al. 2020

Methods in Molecular Biology

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High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae)

et al. 2013

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High-density genetic maps were constructed for loci involved in nuclear male sterility (NMS1-locus) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L.). The mapping population consisted of 389 F1' individuals derived from a cross between two plants, K28 (male-sterile) and K59 (pollen-fertile), both heterozygous at the S-locus. This F1' mapping population segregated for both male sterility (MS) and strong self-incompatibility (SI) phenotypes. Phenotyping F1' individuals for MS allowed us to map the NMS1-locus to linkage group (LG) 5, while controlled diallel and factorial crosses to identify compatible/incompatible phenotypes mapped the S-locus to LG2. To increase the density of markers around these loci, bulked segregant analysis was used. Bulks and parental plants K28 and K59 were screened using amplified fragment length polymorphism (AFLP) analysis, with a complete set of 256 primer combinations of EcoRI-ANN and MseI-CNN. A total of 31,000 fragments were generated, of which 2,350 showed polymorphism between K59 and K28. Thirteen AFLP markers were identified close to the NMS1-locus and six in the vicinity of the S-locus. From these AFLP markers, eight were transformed into sequence-characterized amplified region (SCAR) markers and of these five showed co-dominant polymorphism. The chromosomal regions containing the NMS1-locus and the S-locus were each confined to a region of 0.8 cM. In addition, we mapped genes encoding proteins similar to S-receptor kinase, the female determinant of sporophytic SI in the Brasicaceae, and also markers in the vicinity of the putative S-locus of sunflower, but none of these genes or markers mapped close to the chicory S-locus.

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Identification of AFLP markers linked to Ms, a genic multiple allele inherited male-sterile gene in Chinese cabbage

Cited by 9 publications

References 38 publications

Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

Amplified Fragment Length Polymorphism: Applications and Recent Developments

High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae)

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