Autoradiographic Determination of Neurotransmitter Receptor Distributions in the Cerebral and Cerebellar Cortices,

Wamsley, James K.; Palacios, J.M.; Young, W. Scott; Kuhar, Michael J.

doi:10.1177/29.1a_suppl.6270206

Cited by 41 publications

(3 citation statements)

References 30 publications

(20 reference statements)

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“…(The proenkephalin-immunostained bipolar-like cells and some of the multipolar cells extend their dendrites into layer I.) However, an alternative autoradiographic technique has localized mu-type ligands to layer IV in rats (Goodman et al, 1980;Wamsley et al, 1981), where little or no opioid peptidergic-ir in cells or fibers was found in the present study. Other opioid receptor subtypes have been localized with a predominance in different cortical layers (delta subtype- Goodman et al, 1980;Lewis et al, 1983; kappa subtype- Foote and Maurer, 1982) or different regions (kappa subtype- Goodman and Snyder, 1982;sigma subtype-Quirion et al, 1981;Zukin et al, 1983).…”

Section: Discussioncontrasting

confidence: 65%

The distribution and morphology of opioid peptide immunoreactive neurons in the cerebral cortex of rats

McGinty¹,

Kooy²,

Fe³

1984

J. Neurosci.

118

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Pharmacological and biochemical evidence has implied that a widespread opioid peptide system exists within the cerebral cortex to mediate a variety of opiate effects. However, immunocytochemical detection of opioid peptides in the cortex has been limited. Using antisera to enkephalin and bovine adrenal medullary peptide, both fragments of proenkephalin, and an antiserum to dynorphin A, a fragment of prodynorphin, we now describe the regional and laminar distribution of a widespread population of olfactory cortical and neocortical cell bodies and fibers with opioid immunoreactivity in rats. Neurons stained with each antiserum are distributed bimodally in layers II and III and V and VI of neocortex as well as in layers II and III of olfactory cortex. The widespread distribution and heterogeneous morphology of cortical cells containing proenkephalin and dynorphin-A immunoreactivity suggest that opioid peptide-containing neurons may influence the functioning of local, commissural, and projection neurons in rat cerebral cortex.

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Section: Discussioncontrasting

confidence: 65%

The distribution and morphology of opioid peptide immunoreactive neurons in the cerebral cortex of rats

McGinty¹,

Kooy²,

Fe³

1984

J. Neurosci.

118

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“…The response to OX0 in layers IV and V coincides with the laminar distribution of cholinoceptive cells (Krnjevid and Phillis, 1963a, b), the concentration of acetylcholinesterase (Beesley and Emson, 1975), and the densities of high affinity muscarinic binding sites in the neocortex (Wamsley et al, 1981;J. K. Wamsley, unpublished data).…”

Section: Discussionsupporting

confidence: 52%

Effects of oxotremorine on local glucose utilization in the rat cerebral cortex

Dam¹,

Wamsley²,

Rapoport³

et al. 1982

J. Neurosci.

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The [14C]2-deoxy-D-glucose technique (Sokoloff, L., M. Reivich, C. Kennedy, M. Des Rosiers, C. Patlak, K. Pettigrew, 0. Sakurada, and M. Shinohara (1977) J. Neurochem. 28: 897-916) was used to examine the effects of central muscarinic stimulation on local cerebral glucose utilization (LCGU) in the cerebral cortex of the unanesthetized rat. Systemic administration of the muscarinic agonist oxotremorine (0X0, 0.1 to 1.0 mg/kg, i.p.) increased LCGU in the neocortex, mesocortex, and paleocortex. In the neocortex, OX0 was more potent in elevating LCGU of the auditory, frontal, and sensorimotor regions compared with the visual cortex. Within these neocortical regions, OX0 effects were greatest in cortical layers IV and V. OX0 effects were more dramatic in the neocortex than in the meso-or paleocortex, and no significant effect occurred in the perirhinal and pyriform cortices. 0X0-induced LCGU increases were not influenced by methylatropine (1 mg/kg, s.c.) but were antagonized completely by scopolamine (2.5 mg/kg, i.p.). Scopolamine reduced LCGU in layer IV of the auditory cortex and in the retrosplenial cortex. The distribution and magnitude of the cortical LCGU response to OX0 apparently were related to the distributions of cholinergic neurochemical markers, especially high affinity muscarinic binding sites.

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“…Quantitative receptor autoradiography using emulsion and tritium-sensitive film has been de-scribed for [3H]QNB and other neurotransmitter ligands and provides a higher level of resolution than is possible with homogenate binding studies 13,21,23,26,30,34,35,38,39. Direct comparison of quantitative autoradiographic data and homogenate binding demonstrated consistent, significant absolute differences between the two methods.…”

Section: Discussionmentioning

confidence: 99%

Muscarinic cholinergic receptors in human infant forebrain: [3H]Quinuclidinyl benzilate binding in homogenates and quantitative autoradiography in sections

Johnston

Silverstein

Reindel

et al. 1985

Developmental Brain Research

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The ontogeny of muscarinic receptors in human brain was studied by comparing [3H]quinuclidinyl benzilate ([3H]QNB) binding in postmortem tissue from infants 1 week to 3 months of age with binding in adult specimens. Saturation analysis with [3H]QNB and displacement studies with muscarinic antagonists and agonists in tissue homogenates demonstrated that binding sites in the infants' forebrain regions were present in adult or higher than adult concentrations (Bmax). Binding affinity (Ka) and pharmacological characteristics were nearly identical at the two ages. Quantitative receptor autoradiography demonstrated more [3H]QNB binding in the gray matter of infants than adults and revealed a marked difference between the two ages in the laminar distribution of binding sites in neocortex. In contrast to the adult pattern with higher binding in superficial layers 1-3 than in layers 4-6, the distribution in the immature cortex was inverted. These results suggest that muscarinic receptors in infants resemble closely those in mature brain. However, the topography of receptors in the immature neocortex is distinct and they are redistributed in a gradient from inside outward during postnatal development.

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Autoradiographic Determination of Neurotransmitter Receptor Distributions in the Cerebral and Cerebellar Cortices,

Cited by 41 publications

References 30 publications

The distribution and morphology of opioid peptide immunoreactive neurons in the cerebral cortex of rats

The distribution and morphology of opioid peptide immunoreactive neurons in the cerebral cortex of rats

Effects of oxotremorine on local glucose utilization in the rat cerebral cortex

Muscarinic cholinergic receptors in human infant forebrain: [3H]Quinuclidinyl benzilate binding in homogenates and quantitative autoradiography in sections

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