Autophagy in SDF-1α-mediated DPSC migration and pulp regeneration

Yang, Jingwen; Zhang, Yufeng; Wan, Chao; Sun, Zheyi; Nie, Shuai; Jian, Shu-juan; Zhang, Lu; Song, Gaoyuan; Chen, Zhi

doi:10.1016/j.biomaterials.2014.12.006

Cited by 140 publications

(97 citation statements)

References 47 publications

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“…The similar findings have been reported in another animal study using an orthotopic infection model (Yang et al . ). The root canals of dog premolars with closed apices were chemomechanically instrumented after the periapical lesions were induced.…”

Section: Cell Homing Approachmentioning

confidence: 97%

Regenerative endodontics: a comprehensive review

et al. 2018

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The European Society of Endodontology and the American Association for Endodontists have released position statements and clinical considerations for regenerative endodontics. There is increasing literature on this field since the initial reports of Iwaya et al. (Dental Traumatology, 17, 2001, 185) and Banchs & Trope (Journal of Endodontics, 30, 2004, 196). Endogenous stem cells from an induced periapical bleeding and scaffolds using blood clot, platelet rich plasma or platelet-rich fibrin have been utilized in regenerative endodontics. This approach has been described as a 'paradigm shift' and considered the first treatment option for immature teeth with pulp necrosis. There are three treatment outcomes of regenerative endodontics; (i) resolution of clinical signs and symptoms; (ii) further root maturation; and (iii) return of neurogenesis. It is known that results are variable for these objectives, and true regeneration of the pulp/dentine complex is not achieved. Repair derived primarily from the periodontal and osseous tissues has been shown histologically. It is hoped that with the concept of tissue engineering, namely stem cells, scaffolds and signalling molecules, that true pulp regeneration is an achievable goal. This review discusses current knowledge as well as future directions for regenerative endodontics. Patient-centred outcomes such as tooth discolouration and possibly more appointments with the potential for adverse effects needs to be discussed with patients and parents. Based on the classification of Cvek (Endodontics and Dental Traumatology, 8, 1992, 45), it is proposed that regenerative endodontics should be considered for teeth with incomplete root formation although teeth with near or complete root formation may be more suited for conventional endodontic therapy or MTA barrier techniques. However, much is still not known about clinical and biological aspects of regenerative endodontics.

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Section: Cell Homing Approachmentioning

confidence: 97%

Regenerative endodontics: a comprehensive review

et al. 2018

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“…Autophagy is not only a simple tool for the elimination of cellular materials, but is also an important recycling system for tissue renovation [29-31]. Recently, the role of autophagy in bone physiology and pathophysiology has gradually been unmasked.…”

Section: Discussionmentioning

confidence: 99%

Deferoxamine-Induced Migration and Odontoblast Differentiation via ROS-Dependent Autophagy in Dental Pulp Stem Cells

Wang

Jiang

et al. 2017

Cell Physiol Biochem

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Background/Aims: As a vital degradation and recycling system, autophagy plays an essential role in regulating the differentiation of stem cells. We previously showed that iron chelator deferoxamine (DFO) could promote the repair ability of dental pulp stem cells (DPSCs). Here, we investigated the effect of DFO in autophagy and the role of autophagy in regulating the migration and odontoblast differentiation of DPSCs. Methods: Transmission electron microscopy, immunofluorescence staining and western blotting were performed to evaluate the autophagic activity of DPSCs. Transmigration assay, alkaline phosphatase staining/activity, alizarin red S staining and quantitative PCR were performed to examine the migration and odontoblast differentiation of DPSCs. Reactive oxygen species (ROS) levels and the effects of ROS scavenger in autophagy induction were also detected. Autophagy inhibitors (3-MA and bafilomycin A1) and lentiviral vectors carrying ATG5 shRNA sequences were used for autophagy inhibition. Results: Early exposure to DFO promoted the mineralization of DPSCs and increased autophagic activity. Autophagy inhibition suppressed DFO-induced DPSC migration and odontoblast differentiation. Furthermore, DFO treatment could induce autophagy partly through hypoxia-inducible factor 1α/B cell lymphoma 2/adenovirus E1B 19K-interacting protein 3 (HIF-1α/BNIP3) pathway in a ROS-dependent manner. Conclusion: DFO increased DPSC migration and differentiation, which might be modulated through ROS-induced autophagy.

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“…The application of such growth factors without stem cells, in a cell homing versus cell transplantation strategy, has also been suggested as a more clinically translational approach for dentin-pulp regeneration. Based on this concept, ectopic regeneration of dental pulp-like tissues using basic Fibroblast Growth Factor (b-FGF), Vascular Endothelial Growth Factor (VEGF), or Platelet-Derived Growth Factor (PDGF) with a basal set of Nerve Growth Factor (NGF) and Bone Morphogenetic Protein 7 (BMP-7) has been reported [91], while other researches achieved complete pulp regeneration in pulpectomized mature dog teeth by using a stromal cell-derived factor-1a- (SDF-1a-) loaded silk fibroin scaffold without DPSC transplantation [92]. …”

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou

About

2016

Stem Cells International

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Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade. Key milestone achievements have exemplified their clinical utility in Regenerative Dentistry, as surrogate therapeutic modules for conventional biomaterial-based approaches, offering regeneration of damaged oral tissues instead of simply “filling the gaps.” Thus, the essential next step to validate these immense advances is the implementation of well-designed clinical trials paving the way for exploiting these fascinating research achievements for patient well-being: the ultimate aim of this ground breaking technology. This review paper presents a concise overview of the major biological properties of the human dental MSCs, critical for the translational pathway “from bench to clinic.”

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Autophagy in SDF-1α-mediated DPSC migration and pulp regeneration

Cited by 140 publications

References 47 publications

Regenerative endodontics: a comprehensive review

Regenerative endodontics: a comprehensive review

Deferoxamine-Induced Migration and Odontoblast Differentiation via ROS-Dependent Autophagy in Dental Pulp Stem Cells

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

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