Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou, Athina; About, Imad

doi:10.1155/2016/4209891

Cited by 67 publications

(55 citation statements)

References 192 publications

(273 reference statements)

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“…SCAPs appear to be the source of odontoblasts that are responsible for the formation of root dentin [5,6]. Compared with dental pulp stem cells (DPSCs), they have a higher expression of telomerase, a superior proliferation rate and dental tissue regeneration capacity [6][7][8]. They express surface markers common to other dental stem cells, like STRO-1, but also a distinctive marker, CD24 [9].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties

Rémy

Ferraro

Salver

et al. 2019

Cells

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Stem cells isolated from the apical papilla of wisdom teeth (SCAPs) are an attractive model for tissue repair due to their availability, high proliferation rate and potential to differentiate in vitro towards mesodermal and neurogenic lineages. Adult stem cells, such as SCAPs, develop in stem cell niches in which the oxygen concentration [O 2 ] is low (3-8% compared with 21% of ambient air). In this work, we evaluate the impact of low [O 2 ] on the physiology of SCAPs isolated and processed in parallel at 21% or 3% O 2 without any hyperoxic shock in ambient air during the experiment performed at 3% O 2 . We demonstrate that SCAPs display a higher proliferation capacity at 3% O 2 than in ambient air with elevated expression levels of two cell surface antigens: the alpha-6 integrin subunit (CD49f) and the embryonic stem cell marker (SSEA4). We show that the mesodermal differentiation potential of SCAPs is conserved at early passage in both [O 2 ], but is partly lost at late passage and low [O 2 ], conditions in which SCAPs proliferate efficiently without any sign of apoptosis. Unexpectedly, we show that autophagic flux is active in SCAPs irrespective of [O 2 ] and that this process remains high in cells even after prolonged exposure to 3% O 2 .

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Section: Introductionmentioning

confidence: 99%

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties

Rémy

Ferraro

Salver

et al. 2019

Cells

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“…Compared with other stem cell sources like bone marrow, umbilical cord, or adipose tissue, dental MSCs present several benefits. 49,50 They have a higher proliferation rate and an increased survival rate in culture with a delayed senescence process, compared with BMMSCs. To date, no major adverse effects, such as teratoma formation following in vivo application, have been observed.…”

Section: Perspectivesmentioning

confidence: 99%

Clinical application of mesenchymal stem cells in periodontal regeneration: A systematic review and meta‐analysis

Novello

Debouche

Marie

et al. 2019

J of Periodontal Research

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Objective To evaluate the potential efficacy of mesenchymal stem cells (MSCs) in periodontal regeneration in humans on the following main outcomes: clinical attachment level (CAL), probing depth (PD), and gingival recession (GR). Background The clinical application of stem cells in periodontal regeneration has begun in recent years, but clinical practices are not yet standardized and no recommendations are available at this time. Methods Electronic database searches and hand searches were conducted. All types of studies, case series, and case reports were qualitatively described. Double‐blind randomized controlled trials (RCTs) evaluating MSCs in periodontal regeneration were included in a meta‐analysis if they compared administration of MSCs vs application of stem cell‐free therapy in the control group, in healthy patients with periodontal defects, with a minimum of three mo of follow‐up. Results Fifteen reports were included in qualitative analysis, involving 123 patients and 158 periodontal defects. Only two small RCTs at high risk of bias, with a total of 59 patients and 70 periodontal defects, were included in the meta‐analysis. A small but significant difference between test and control groups was found for CAL at three mo (−0.90 mm, 95% CI [−1.51; −0.29]), but not for PD and GR. Conclusion Low‐quality evidence suggests that MSC‐based therapy may have a small impact on periodontal regeneration. However, due to the monocentric character, the small sample size, and potential heterogeneity across the two included RCTs, these results must not be considered as definitive. High‐quality RCTs are needed before any clinical use of MSCs in periodontal regeneration.

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“…However, their differentiation ability and in vivo regenerative potential remains unclear. 50 In summary, the multipotency, increased proliferation rates, and ease of accessibility makes dental stem cells an attractive source for tissue regeneration. Next, selected studies involving the use of dental stem cells and advanced scaffolds for dental pulp and periodontal regeneration are discussed.…”

Section: Dental Stem Cellsmentioning

confidence: 99%

Advanced Scaffolds for Dental Pulp and Periodontal Regeneration

Bottino

Pankajakshan

Nör

2017

Dental Clinics of North America

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Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Cited by 67 publications

References 192 publications

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties

Clinical application of mesenchymal stem cells in periodontal regeneration: A systematic review and meta‐analysis

Advanced Scaffolds for Dental Pulp and Periodontal Regeneration

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