In this study, we propose a technological system designed to provide machine vision-based automatic inspection and autonomous operation services for an entire process related to product inspection in wire harness manufacturing. The smart factory paradigm is a valuable and necessary goal, small companies may encounter steep barriers to entry. Therefore, the best approach is to develop towards this approach gradually in stages starting with the relatively simple improvement to manufacturing processes, such as replacing manual quality assurance stages with machine vision-based inspection. In this study, we consider design issues of a system based on the proposed technology and describe an experimental implementation. In addition, we evaluated the implementation of the proposed technology. The test results show that the adoption of the proposed machine vision-based automatic inspection and operation service system for multi-wire harness production may be considered justified, and the effectiveness of the proposed technology was verified.