Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry

Chaturvedi, Akhil; Gorthi, Sai Siva

doi:10.1177/2211068216663604

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…For the next advance, microfluidic-based techniques have emerged in recent years as an approach to detect biological matter in fluidic samples [58,59]. These techniques allow for miniaturized solutions with a lab-on-chip approach with reduced costs and high resolution.…”

Section: Water Sample Analysesmentioning

confidence: 99%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.

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Section: Water Sample Analysesmentioning

confidence: 99%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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“…This is often achieved with the LAMP test and its derivatives, LAMPport and NINA-LAMP [22,47,48,55,61]. However, many other, especially microfluidic, approaches have also been tested and discussed for their aptitude to be used for POC diagnostic tools [38,45,205,212,[221][222][223][224][225].…”

Section: Point-of-care Diagnostics (Poc)mentioning

confidence: 99%

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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“…Figure 4 a shows the schematic of a PDMS-based FC microfluidic device and its fabrication process [ 94 ]. Over the years, the development of polymer-based microfluidics drives from the FC devices evolving from ‘benchtop’ to integrated chips, making them much portable and affordable [ 95 , 96 ].…”

Section: The Application Of Polymer-based Microfluidics For Singlementioning

confidence: 99%

Microfluidic-Based Single-Cell Study: Current Status and Future Perspective

Zhu

Huang

et al. 2018

Molecules

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Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic devices have played an increasingly important role in the field of single-cell study owing to their advantages: high efficiency, easy operation, and low cost. In this review, the applications of polymer-based microfluidics on cell manipulation, cell treatment, and cell analysis at single-cell level are detailed summarized. Moreover, three mainly types of manufacturing methods, i.e., replication, photodefining, and soft lithography methods for polymer-based microfluidics are also discussed.

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Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry

Cited by 8 publications

References 21 publications

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Microfluidic-Based Single-Cell Study: Current Status and Future Perspective

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