Autologous non-cultured epidermal cell suspension combined with platelet rich fibrin for the treatment of stable vitiligo: A case series

Abstract

Non-cultured epidermal cell suspension (NCECS) is a relatively new cellular grafting technique for vitiligo. Platelet rich fibrin (PRF) is a platelet and immune concentrate gather on a single fibrin membrane which can be used in conjunction with grafts and has several advantages, such as promoting wound healing, haemostasis, and give better handling properties to graft materials. This study was conducted to determine the efficacy of NCECS combined with PRF in patients with stable vitiligo. Seven patients with stable vitiligo which not responding to topical and phototherapy for more than 12 months were included in the study. The melanocytes were harvested as an autologous melanocyte rich suspension from a donor skin. The non cultured melanocyte transplanted to recipient area that had been superficially dermabraded and smeared with PRF gel. Of all 7 patients, 1 patients showed excellent pigmentation (90-100%), 2 had good repigmentation (60-89%), 1 had fair repigmentation (25-59%) and 3 patients had a poor response (0-24%). The procedure is safe and promising surgical modality for stable vitiligo.

Autologous noncultured, nontrypsinized melanocyte‐keratinocyte graft homogenized in plasma gel followed by narrow‐band ultraviolet B therapy for stable vitiligo: A novel technique

“…It acts as a trap for presynthesized growth factors which promote melanogenesis 19 and enhances wound healing, proliferation, and regeneration of cells and melanocyte stabilization through the effect of fibroblast growth factor which stimulates keratinocytes and fibroblasts proliferation with subsequent improvement of their interaction with melanocytes and improving angiogenesis 20‐22 . This was supported by the findings of previous studies 23‐25 . In addition, plasma gel is known for its antibacterial effect which covers a broad spectrum of bacteria including gram positive and gram negative bacteria 26‐28 so it protected the recipient site from infection instead of topical antibiotics which may affect melanocytes viability.…”

“…[20][21][22] This was supported by the findings of previous studies. [23][24][25] In addition, plasma gel is known for its antibacterial effect which covers a broad and nonmelanoma skin cancers. [29][30][31] The present technique agreed with cultured melanocyte transplantation [32][33][34] and noncultured trypsinized epidermal cell suspension techniques 7,[32][33][34][35] in the ability of all cellular grafting techniques to induce uniform and homogenized repigmentation with a relatively equal efficacy, but, it was different from the cultured techniques [32][33][34] in not being time consuming, not expensive as it does not need specific culture media, and well-equipped laboratory which is needed to be maintained over several weeks.…”

Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa

“…However, this procedure has been widely used for treating burns, chronic wounds, and even vitiligo. 11 , 15 , 16…”

“…Also, the injection of fibroblasts into wounded skin can be very painful, which limits its clinical application. 9 , 10 Based on these findings, we aimed to modify our cell-therapy, 11 using a non-cultured cell suspension spray applied to JEB chronic wounds to improve the adherence of keratinocytes. In addition, the occlusion of 10% silver sulfadiazine using plastic wrap was applied afterwards to induce epithelization.…”