Autologous Blood Vessels Engineered from Peripheral Blood Sample

Aper, Thomas; Schmidt, Andrej; Duchrow, M.; Bruch, Hans–Peter

doi:10.1016/j.ejvs.2006.08.008

Cited by 62 publications

(23 citation statements)

References 41 publications

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“…in vitro functional studies provide a foundation for in vivo animal studies as well as eventual clinical applications. Although there are a few studies that have investigated the feasibility of using endothelial-like cells to engineer a vascular graft, the characterization of the resulting endothelial-like cells is very limited [5,6,13,18,19]. In particular, there is a dearth of information on the antithrombogenic and fibrinolytic function of these cells, especially when cultured on a biomaterial.…”

Section: Introductionmentioning

confidence: 99%

Toward Engineering a Human Neoendothelium with Circulating Progenitor Cells

et al. 2009

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Tissue-engineered vascular grafts may one day provide a solution to many of the limitations associated with using synthetic vascular grafts. However, identifying a suitable cell source and polymer scaffold to recreate the properties of a native blood vessel remains a challenge. In this work, we assess the feasibility of using endothelial progenitor cells (EPCs) found in circulating blood to generate a functional endothelium on poly(1,8-octanediol-co-citrate) (POC), a biodegradable elastomeric polyester. EPCs were isolated from human blood and biochemically differentiated into endothelial-like cells (HE-like) in vitro. The differentiated cell phenotype and function was confirmed by the appearance of the characteristic endothelial cell (EC) cobblestone morphology and positive staining for EC markers, von Willebrand factor, vascular endothelial cadherin, flk-1, and CD31. In addition, HE-like cells cultured on POC express endothelial nitric oxide synthase at levels comparable to aortic ECs. Furthermore, as with mature endothelial cells, HE-like cell populations show negligible expression of tissue factor. Similarly, HE-like cells produce and secrete prostacyclin and tissue plasminogen activator at levels comparable to venous and aortic ECs. When compared to fibroblast cells, HE-like cells cultured on POC show a decrease in the rate of plasma and wholeblood clot formation as well as a decrease in platelet adhesion. Finally, the data show that HE-like cells can withstand physiological shear stress of 10 dynes/cm 2 when cultured on POC-modified expanded poly(tetrafluoroethylene) vascular grafts. Collectively, these data are the foundation for future clinical studies in the creation of an autologous endothelial cell-seeded vascular graft. STEM CELLS 2010;28:318-328 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Introductionmentioning

confidence: 99%

Toward Engineering a Human Neoendothelium with Circulating Progenitor Cells

et al. 2009

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“…[294] Fibrin (1) Non toxic, non inflammatory, [302] non allergenic, [303] non immunogenic [304], economical, [305] biodegradable [306] and easily processable. [307] (2)…”

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confidence: 99%

Experimental approaches to vascularisation within tissue engineering constructs

Sarker

Chen

Schreyer

2015

Journal of Biomaterials Science, Polymer Edition

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Tissue engineering opens up a new area to restore the function of damaged tissue or replace a defective organ. Common strategies in tissue engineering to repair and form new tissue containing a functional vascular network include the use of cells, growth factors, extracellular matrix proteins, and biophysical stimuli. Yet, formation of well-distributed, interconnected, and stable vascular network still remains challenging. In addition, anastomoses with host vasculature upon implantation and long-time survival of the new blood vessel in vivo are other critical issues to be addressed. This paper presents a brief review of recent advances in vascularization in vitro as well as in vivo for tissue engineering, along with suggestions for future research.

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“…Fibrin gel has been studied as a scaffold for tissue-engineered vascular grafts (TEVG) [3][4][5][6]. An advantage of fibrin is that it can be produced from a patient's own blood and used as an autologous scaffold without the risk of a foreign body reaction.…”

Section: Introductionmentioning

confidence: 99%