Human coronary artery smooth muscle cell response to a novel PLA textile/fibrin gel composite scaffold

Gundy, Sarah L.; Manning, Grainne; O’Connell, Enda; Ellä, Ville; Harwoko, Marvi Sri; Rochev, Yuri; Smith, Terry J.; Barron, Valerie

doi:10.1016/j.actbio.2008.05.025

Cited by 30 publications

(19 citation statements)

References 28 publications

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“…The unique potential of fibrin as a scaffold has been well documented. 2,9,13,19,23,33,37 The necessity to optimize the concentrations of fibrin scaffold components 7,14 and fibrinolytic inhibitors 15 has been highlighted previously. As a result, the seeding technique and the fibrin component concentrations used in this study were based on those previously optimized for tissue engineering vascular grafts.…”

Section: Introductionmentioning

confidence: 96%

“…As a result, the seeding technique and the fibrin component concentrations used in this study were based on those previously optimized for tissue engineering vascular grafts. 13,34,37,40 MSC-seeded scaffolds were examined for a range of seeding densities in terms of gel compaction, MSC viability, metabolic activity, multipotency, and collagen formation. Thereafter, changes in gene expression of SMC-associated markers, including alpha-smooth muscle actin (a-SMA) and calponin, were examined using real-time quantitative PCR (qPCR).…”

Section: Introductionmentioning

confidence: 99%

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Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

et al. 2010

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A limitation of current tissue engineering vascular graft technology is the provision of an expandable, autologous cell source. By harnessing the multipotency of mesenchymal stem cells (MSC), it is hoped that functional vascular cells can be produced. To date, a range of 2D and 3D environments have been investigated for the manipulation of MSC differentiation pathways. To this end, this study aims to test the hypothesis that MSC seeded in various fibrin gel environments will exhibit evidence of a smooth muscle cell (SMC) phenotype. Initially, a range of cell-seeding densities were screened for 2D and 3D fibrin constructs, where it was observed that a seeding densities of 500,000 cells/mL facilitated gel compaction without degradation or loss in cell viability. Additionally, positive expression of CD49, CD73, CD105 markers and negative expression of hemopoietic stem cell-associated CD34 and CD45 indicated that MSC phenotype was retained within the fibrin gel. Nonetheless, a decrease in the gene expression of alpha-smooth cell actin and calponin was observed for MSC cultured in static 3D fibrin gels. Although a slight recovery was observed after 24 h mechanical stimulation, the fold-change remained significantly lower than that observed for cells cultured on 2D tissue culture plastic. While MSC differentiation toward a SMC appears possible in both 2D and 3D environments, scaffold architecture and mechanical stimulation undoubtedly play an important role in the creation of a functional SMC phenotype.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

et al. 2010

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“…Moreover, their non-cytotoxicity, their ease to use with many fabrication techniques and often the simplicity of their synthesis makes them to be widely used within the field of biomaterials [4–5 55,85,99–102]. Examples of the most representative synthetic polymeric materials used for vascular cell studies are poly(dimethylsiloxane) (PDMS) [6,61,103–106], poly(ethylene glycol) (PEG)-derived polymers [5,87,98,107–113] poly(acrylamide) (PAA) [50,114–116] and poly(lactic acid) (PLA) [117–118]. Alternative materials used for micro- and nanostructuring are glass [119] , ceramics [119–122] or natural polymers that can also be synthetically modified by, e.g., functionalizing with an artificial polymeric group [113,123].…”

Section: Reviewmentioning

confidence: 99%

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

Greiner¹,

Sales²,

Chen³

et al. 2016

Beilstein J. Nanotechnol.

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The extracellular environment of vascular cells in vivo is complex in its chemical composition, physical properties, and architecture. Consequently, it has been a great challenge to study vascular cell responses in vitro, either to understand their interaction with their native environment or to investigate their interaction with artificial structures such as implant surfaces. New procedures and techniques from materials science to fabricate bio-scaffolds and surfaces have enabled novel studies of vascular cell responses under well-defined, controllable culture conditions. These advancements are paving the way for a deeper understanding of vascular cell biology and materials–cell interaction. Here, we review previous work focusing on the interaction of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) with materials having micro- and nanostructured surfaces. We summarize fabrication techniques for surface topographies, materials, geometries, biochemical functionalization, and mechanical properties of such materials. Furthermore, various studies on vascular cell behavior and their biological responses to micro- and nanostructured surfaces are reviewed. Emphasis is given to studies of cell morphology and motility, cell proliferation, the cytoskeleton and cell-matrix adhesions, and signal transduction pathways of vascular cells. We finalize with a short outlook on potential interesting future studies.

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“…(24,48 and 72 h) were 20 and 19, respectively. Of these genes, those included in the GO category of 'Biological process' whose expression level had increased were classified into such functions as cell differentiation, cell death, apoptosis, and cholesterol metabolism.…”

Section: Methods For Grouping Differentially Expressed Genes Using Bimentioning

confidence: 99%

“…(ii) The investigator groups differentially expressed genes on the basis of such criteria as gene function, and from these gene groups infers differences in cell function depending on the material [37][38][39][40][41][42]. (iii) Differences in cell function, depending on the material, are predicted from differentially expressed gene groups using such bioinformatics techniques as Gene Ontology (GO), and from the functions of these gene groups [43][44][45][46][47][48].…”

Section: Comparison Of Gene Expression Between Two Types Of Materialsmentioning

confidence: 99%

Global gene expression analysis for evaluation and design of biomaterials

Hanagata

Takemura

Minowa

2010

Science and Technology of Advanced Materials

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Comprehensive gene expression analysis using DNA microarrays has become a widespread technique in molecular biological research. In the biomaterials field, it is used to evaluate the biocompatibility or cellular toxicity of metals, polymers and ceramics. Studies in this field have extracted differentially expressed genes in the context of differences in cellular responses among multiple materials. Based on these genes, the effects of materials on cells at the molecular level have been examined. Expression data ranging from several to tens of thousands of genes can be obtained from DNA microarrays. For this reason, several tens or hundreds of differentially expressed genes are often present in different materials. In this review, we outline the principles of DNA microarrays, and provide an introduction to methods of extracting information which is useful for evaluating and designing biomaterials from comprehensive gene expression data.

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Human coronary artery smooth muscle cell response to a novel PLA textile/fibrin gel composite scaffold

Cited by 30 publications

References 28 publications

Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

Global gene expression analysis for evaluation and design of biomaterials

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