Experimental approaches to vascularisation within tissue engineering constructs

Sarker, Md; Chen, Daniel; Schreyer, David J.

doi:10.1080/09205063.2015.1059018

Cited by 56 publications

(61 citation statements)

References 345 publications

(339 reference statements)

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“…The success of a macro-scale tissue engineered construct depends on several factors, including the availability of a mass diffusion network within 100–200 µm from the cell population, uniform distribution of multiple cell types with reasonable density, and nonthrombogenic phenotype of ECs upon integration with the host vasculature [4] , [70] . To address this issue, researchers have taken a bottom-up approach to fabricate macro-scale scaffolds, and found that self-assembled micro-tissues or -modules were a possible solution for fabricating large engineered constructs, as shown in Fig.…”

Section: Assembled Scaffoldsmentioning

confidence: 99%

“…In particular, the vascular network should reach within 100–200 µm of the tissue to avoid ischemic conditions and cell death [3] . Blood vessels with different diameters (∼ 4–300 µm) spread in a complicated fashion (i.e., fractal shapes) into tissue to exchange nutrients, gas, and metabolites to a huge cell population [4] . Capillaries in the vascular network play a vital role in the mass transfer mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…Tissue vascularization is a complex process that develops through vasculogenesis and angiogenesis in vivo [4] , [5] , [6] . In vasculogenesis, endothelial progenitor cells (EPCs) migrate to an ischemic site, proliferate, and differentiate to form capillary vessels, while angiogenesis occurs when new blood vessels sprout from existing ones according to a gradient of angiogenic factors [5] , [7] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

3D biofabrication of vascular networks for tissue regeneration: A report on recent advances

Sarker

Naghieh

Sharma

et al. 2018

Journal of Pharmaceutical Analysis

137

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Rapid progress in tissue engineering research in past decades has opened up vast possibilities to tackle the challenges of generating tissues or organs that mimic native structures. The success of tissue engineered constructs largely depends on the incorporation of a stable vascular network that eventually anastomoses with the host vasculature to support the various biological functions of embedded cells. In recent years, significant progress has been achieved with respect to extrusion, laser, micro-molding, and electrospinning-based techniques that allow the fabrication of any geometry in a layer-by-layer fashion. Moreover, decellularized matrix, self-assembled structures, and cell sheets have been explored to replace the biopolymers needed for scaffold fabrication. While the techniques have evolved to create specific tissues or organs with outstanding geometric precision, formation of interconnected, functional, and perfused vascular networks remains a challenge. This article briefly reviews recent progress in 3D fabrication approaches used to fabricate vascular networks with incorporated cells, angiogenic factors, proteins, and/or peptides. The influence of the fabricated network on blood vessel formation, and the various features, merits, and shortcomings of the various fabrication techniques are discussed and summarized.

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Section: Assembled Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

3D biofabrication of vascular networks for tissue regeneration: A report on recent advances

Sarker

Naghieh

Sharma

et al. 2018

Journal of Pharmaceutical Analysis

137

View full text Add to dashboard Cite

show abstract

“…Despite being valid approaches, these strategies present some weaknesses. Indeed, pitfalls in i) matching growth factor type and time-releasing profile 10 , ii) identifying the proper cell types and their ratio 11 , and iii) selecting suitable fluid shear stresses (SS) within the micro-scaffold 12 are still unsettled. Moreover, an in vitro 3D model able to summarize the key components of the angiogenic process, like the dynamic interplay between EC and other vascular/mural cells (e.g.…”

Section: Introductionmentioning

confidence: 99%

Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Cerino

Gaudiello

Muraro

et al. 2017

Sci Rep

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In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds. Perfusion culture of SVF-cells reproducibly promoted in vitro the early formation of a capillary-like network, embedded within an ECM backbone, and the release of numerous pro-angiogenic factors. Compared to static cultures, perfusion-based engineered constructs were more rapidly vascularized and supported a superior survival of delivered cells upon in vivo ectopic implantation. This was likely mediated by pericytes, whose number was significantly higher (4.5-fold) under perfusion and whose targeted depletion resulted in lower efficiency of vascularization, with an increased host foreign body reaction. 3D-perfusion culture of SVF-cells leads to the engineering of a specialized milieu, here defined as an angiogenic niche. This system could serve as a model to investigate multi-cellular interactions in angiogenesis, and as a module supporting increased grafted cell survival in regenerative medicine.

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“…One aim of tissue engineering is to develop tissue/organ substitutes or scaffolds, based on the principles of biology and engineering, for the repair or replacement of damaged tissues and organs [1,2]. For this, scaffolds, typically of a three-dimensional (3D) porous structure made from biomaterials, play an important role in supporting and/or promoting cell growth, tissue regeneration, and transport of nutrients and wastes.…”

Section: Introductionmentioning

confidence: 99%

Modeling of the Mechanical Behavior of 3D Bioplotted Scaffolds Considering the Penetration in Interlocked Strands

et al. 2018

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Three-dimensional (3D) bioplotting has been widely used to print hydrogel scaffolds for tissue engineering applications. One issue involved in 3D bioplotting is to achieve the scaffold structure with the desired mechanical properties. To overcome this issue, various numerical methods have been developed to predict the mechanical properties of scaffolds, but limited by the imperfect representation of one key feature of scaffolds fabricated by 3D bioplotting, i.e., the penetration or fusion of strands in one layer into the previous layer. This paper presents our study on the development of a novel numerical model to predict the elastic modulus (one important index of mechanical properties) of 3D bioplotted scaffolds considering the aforementioned strand penetration. For this, the finite element method was used for the model development, while medium-viscosity alginate was selected for scaffold fabrication by the 3D bioplotting technique. The elastic modulus of the bioplotted scaffolds was characterized using mechanical testing and results were compared with those predicted from the developed model, demonstrating a strong congruity between them. Once validated, the developed model was also used to investigate the effect of other geometrical features on the mechanical behavior of bioplotted scaffolds. Our results show that the penetration, pore size, and number of printed layers have significant effects on the elastic modulus of bioplotted scaffolds; and also suggest that the developed model can be used as a powerful tool to modulate the mechanical behavior of bioplotted scaffolds.

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Experimental approaches to vascularisation within tissue engineering constructs

Cited by 56 publications

References 345 publications

3D biofabrication of vascular networks for tissue regeneration: A report on recent advances

3D biofabrication of vascular networks for tissue regeneration: A report on recent advances

Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Modeling of the Mechanical Behavior of 3D Bioplotted Scaffolds Considering the Penetration in Interlocked Strands

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