Autohydrolysed Tilapia nilotica Fish Viscera as a Peptone Source in Bacteriocin Production

Abstract

Fish processing generates large amounts of solid and liquid wastes. Many different by-products have been produced from fish processing wastes. Studies on solubilization of Bolti fish (Tilapia nilotica) viscera by endogenous enzymes at different pHs are described. Hydrolysis reactions were conducted with freshly thawed viscera utilizing an initial temperature gradient and terminated at various time points by heat inactivation of the enzymes. Various peptones obtained from hydrolysed visceral homogenates of Bolti fish residues showed their suitability for promoting the growth of lactic acid bacteria (mainly Lactobacillus sake Lb 706), microorganisms with particularly complex nutritional requirements especially peptidic sources. The assay of several treatments with L. sakei Lb 706, producer of the bacteriocin sakacin A, demonstrated that optimum conditions for biomass and bacteriocin production only imply a brief autohydrolysis at room temperature. The results showed that the Bolti fish hydrolysates gave remarkable results to those found in costly commercial media, specifically recommended for culturing and large-scale production of lactic acid bacteria.

Production of Bioactive Peptides from Lactic Acid Bacteria: A Sustainable Approach for Healthier Foods

“…The fish processing industry generates large quantity of liquid and solid wastes, and due to their high protein content, they have been proposed as alternative for preparation of valuable products (Kim & Wijesekara, ; Ferraro et al., ; Rustad, Storrø, & Slizyte, ). Peptones obtained from fish‐processing waste have demonstrated that can produce high amounts of bacteriocins from L. sakei Lb 706 (Deraz, El‐Fawal, Abd‐Ellatif, & Khalil, ), up to 500% more than the quantity obtained using commercial media. Also using peptone derived from fish waste products have demonstrated to support LAB growth and metabolic necessities (Vázquez, Docasal, Prieto, González, & Murado, ), with better results than the gold standard media (MRS; Safari et al., ).…”

Valorisation of effluents obtained from chemical and enzymatic chitin production of Illex argentinus pen by-products as nutrient supplements for various bacterial fermentations

“…A previous study comparing several peptones from different origins, denominations and commercial brands led to a wide range of productive yields for the formation of bacteriocins and the growth of lactic acid bacteria [35]. In this context, protein substrates extracted from tuna heads and cod or tilapia viscera with varying degrees of hydrolysis have been reported as fundamental ingredients for complex media [14,37,38].…”

Characteristics and Use of Peptones from Catfish (Clarias gariepinus) and Pangas Catfish (Pangasius pangasius) Heads as Bacterial Growth Media

“…Moreover, Poernomo and Buckle (2002) reported that peptone isolated from cowtail ray (Trygon sephen) viscera was able to support the microbial growth rate of Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae cultures. Deraz et al (2011) used tilapia viscera for peptone production and showed a high biomass production. Srikandace et al (2017) reported that peptone extracted from kerong fish (Terapon jarbua) showed a higher growth rate in E. coli compared to commercial peptone.…”