Production of Bioactive Peptides from Lactic Acid Bacteria: A Sustainable Approach for Healthier Foods

Venegas-Ortega, María Georgina; Flores‐Gallegos, Adriana C.; Martínez-Hernández, José L.; Aguilar, Cristóbal N.; Nevárez-Moorillón, Guadalupe Virginia

doi:10.1111/1541-4337.12455

Cited by 110 publications

(66 citation statements)

References 155 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the recognition of the gut microbiota, probiotics can be used to modify health and disease, even though the exact mechanisms remain unclear. LAB probiotics are widely used in the food industry and have gradually developed into relatively indispensable functional foods that are eaten on a daily basis (Filanniono, Cagno, & Gobbetti, 2018;Venegas-Ortega, Flores-Gallegos, Martinez-Hernandez, Aguilar, & Nevarez-Moorillion, 2019). Studies have shown that LAB can alter the sensation and motility of the gut (Quigley, 2007;Rousseaux et al, 2007), soften the stool, and change secretion, as well as produce lactic acid and/or short-chain fatty acids that can lower the intraluminal pH and promote defecation (Salminen & Salminen, 1997;Waller et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite‐induced constipation in Kunming mice

Gan

Liang

Diao

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

Constipation is a common clinical manifestation of digestive system disorders and occurs worldwide. This study investigated the ability of Lactobacillus plantarum KSFY06 (LP‐KSFY06) to promote the action of geniposide in preventing montmorillonite‐induced constipation in Kunming mice, with the aim of providing a successful solution. The effects of LP‐KSFY06 and geniposide on constipation were measured, and the results showed that the protective effect of geniposide on constipation was enhanced by LP‐KSFY06 and that the combination resulted in increased weight, moisture content, and particle number of feces. The first black stool defecation time was decreased from 182 min to 87 min, which clearly indicates that defecating difficulty was alleviated in constipated mice. The synergic intervention of LP‐KSFY06 and geniposide (LP + G) assisted in maintaining the body weight of constipated mice. The LP + G intervention significantly increased serum levels of motilin (MTL, 167.8 pg/ml), acetylcholinesterase (AChE, 45.3 pg/ml), substance P (SP, 61.0 pg/ml), vasoactive intestinal peptide (VIP, 70.5 pg/ml), endothelin‐1 (ET‐1, 16.1 pg/ml), and gastrin (73.0 pg/ml) and remarkably decreased somatostatin (SS, 35.2 pg/ml) when compared to those indexes in the LP‐KSFY06 group and geniposide group. The LP + G treatment also significantly increased the mRNA expression of cluster of differentiation 117 (c‐Kit), stem cell factor (SCF), glial cell‐derived neurotrophic factor (GDNF), and remarkably downregulated the expression of inducible nitric oxide synthase (iNOS), transient receptor potential vanilloid‐1 (TRPV1), and cyclooxygenase‐2 (COX‐2). The experimental results showed that the combination treatment has the strongest prevention effect against constipation, and LP‐KSFY06 promotes the ability of geniposide to prevent constipation. Therefore, LP‐KSFY06 is a potential probiotic strain with the capacity to prevent montmorillonite‐induced constipation.

show abstract

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite‐induced constipation in Kunming mice

Gan

Liang

Diao

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…The proteolytic action of LAB strains can be used to obtain bioactive peptides that could participate as regulators in physiological processes [4][5][6]. Although the antimicrobial, proteolytic, and lipolytic properties of LAB have been extensively reported, the selection of strains that have more than one capacity can lead to the detection of multifunctional LAB strains, as we reviewed previously [42]. Antioxidant activities were demonstrated by the inhibition of the ABTS + and DPPH + radicals, using the supernatants obtained by presumptive LAB fermentation of casein-peptone broth.…”

Section: Discussionmentioning

confidence: 99%

Multi-Functional Potential of Presumptive Lactic Acid Bacteria Isolated from Chihuahua Cheese

Venegas-Ortega

Flores‐Gallegos

Aguilar

et al. 2020

Foods

Self Cite

View full text Add to dashboard Cite

The multifunctional properties of autochthonous lactic acid bacteria can be of use for enhancing the sensorial properties of food, as well as in food preservation. An initial screening for antimicrobial, proteolytic, and lipolytic capacities was done in 214 presumptive lactic acid bacteria isolates obtained from Chihuahua cheese manufacturing and during a ripening period of nine months. The antimicrobial screening was done by spot-on-the-lawn tests, using Listeria monocytogenes and Escherichia coli as indicator microorganisms; proteolysis was tested in casein-peptone agar and lipolysis in Mann–Rogosa–Sharpe (MRS)-tributyrin agar. More than 90% of the isolates hydrolyzed the casein, but only 30% hydrolyzed tributyrin; the inhibition of L. monocytogenes in the spot-on-the-lawn assay was used to select 39 isolates that had a bigger inhibition zone (>11.15 mm ± 0.3) than the control (Nisin producer Lactococcus lactis BS-10 Chr Hansen). The selected isolates were grown in MRS to obtain the neutralized cell-free supernatants and verify their antimicrobial activity by agar diffusion and the percentage of growth inhibition techniques. The selected isolates were also growth in casein peptone broth, and the cell-free supernatants were used for the determination of antioxidant activity by the radical scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) techniques. The results were analyzed to identify similarities by cluster analysis, based on their antimicrobial and antioxidant capacities. The isolates were arranged into six clusters; one cluster that included 12 isolates demonstrated L. monocytogenes (784–2811 mm2/mL AU by agar diffusion assay) and E. coli (41%–47% growth inhibition) antimicrobial activity. The isolates clustered in these groups also showed competitive inhibition of both radicals (11%–19% of DPPH and 50%–60% of ABTS). The isolates from cluster one were also identified by 16S rDNA amplification and were identified as Enterococcus faecium. Traditional products such as Chihuahua cheese can be a source or lactic acid bacteria with metabolic properties that can be used in food preparation and preservation.

show abstract

“…The prevailing genera of LAB that are used as probiotics are Lactobacillus , Bifidobacterium , Streptococcus , Enterococcus , and Pediococcus [ 142 , 143 ]. The GRAS status of lactic acid by Food and Drug Administration (FDA) has been extensively utilised in food, dairy, and pharmaceutical industries [ 144 , 145 ]. For instance, Lactobacillus delbrueckii subsp.…”

Section: Potential Of Lactobacillus In Keeping Vagmentioning

confidence: 99%

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

2020

View full text Add to dashboard Cite

Human vagina is colonised by a diverse array of microorganisms that make up the normal microbiota and mycobiota. Lactobacillus is the most frequently isolated microorganism from the healthy human vagina, this includes Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. These vaginal lactobacilli have been touted to prevent invasion of pathogens by keeping their population in check. However, the disruption of vaginal ecosystem contributes to the overgrowth of pathogens which causes complicated vaginal infections such as bacterial vaginosis (BV), sexually transmitted infections (STIs), and vulvovaginal candidiasis (VVC). Predisposing factors such as menses, pregnancy, sexual practice, uncontrolled usage of antibiotics, and vaginal douching can alter the microbial community. Therefore, the composition of vaginal microbiota serves an important role in determining vagina health. Owing to their Generally Recognised as Safe (GRAS) status, lactobacilli have been widely utilised as one of the alternatives besides conventional antimicrobial treatment against vaginal pathogens for the prevention of chronic vaginitis and the restoration of vaginal ecosystem. In addition, the effectiveness of Lactobacillus as prophylaxis has also been well-founded in long-term administration. This review aimed to highlight the beneficial effects of lactobacilli derivatives (i.e. surface-active molecules) with anti-biofilm, antioxidant, pathogen-inhibition, and immunomodulation activities in developing remedies for vaginal infections. We also discuss the current challenges in the implementation of the use of lactobacilli derivatives in promotion of human health. In the current review, we intend to provide insights for the development of lactobacilli derivatives as a complementary or alternative medicine to conventional probiotic therapy in vaginal health.

show abstract

Production of Bioactive Peptides from Lactic Acid Bacteria: A Sustainable Approach for Healthier Foods

Cited by 110 publications

References 155 publications

Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite‐induced constipation in Kunming mice

Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite‐induced constipation in Kunming mice

Multi-Functional Potential of Presumptive Lactic Acid Bacteria Isolated from Chihuahua Cheese

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

Contact Info

Product

Resources

About