Autochthonous white rot fungi from the tropical forest: Potential of Cuban strains for dyes and textile industrial effluents decolourisation

Abstract

Nineteen strains of wood-inhabiting pores fungi, representing thirteen species and ten genera, collected from both natural and "anthropic" (urban) ecosystems in Cuba were tested for their ability to decolourise the industrial anthraquinonic dye Acid Blue 62 (AB 62) in laboratory conditions, in both solid and liquid media. On the basis of their decolourisation rate and growth inhibition, seven strains viz. Tinctoporellus epimiltinus, Trametes maxima, Perenniporia tephropora, Coriolopsis rigida, Hexagonia tenuis, Pachykytospora alabamae and Hexagonia hydnoides were selected for further studies. All the strains were able to decolourise partially or completely the AB62 dye added to Malt extract. Almost total decolourisation was obtained with T. maxima. Decolourising activity was also shown with an industrial textile effluent, containing Remazol Yellow RGB, Remazol Red RR, and Remazol Black B 133. Production of laccase, a ligninolytic enzyme possibly involved in decolourisation, was stimulated by AB 62 for all the strains tested; T. maxima showing the highest production. Lignin peroxidase and manganese peroxidase were not produced under the experimental conditions used. T. maxima could represent a potential candidate for biotechnological applications. The exploitation of local biodiversity in tropical area appears as a potentially productive approach for identifying promising microbial strains for industrial use.

Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?

“…For all these reasons, the WRF that grow in such kind of ecosystems are exposed to a whole range of chemical compounds (Lorenzo and Lázaro, 2003). These fungi should be adapted also to conditions of high temperatures and low humidity in comparison with natural ecosystems (Sanchez et al, 2008). …”

“…However, there have been less investigations attempting to exploit directly local biodiversity (Pointing et al, 2000; Sanchez et al, 2008). However, this approach appears to be potentially productive for identifying new, promising strains for biotechnological applications (Pointing et al, 2003).…”

Mycelial biomass cultivation of Lentinus crinitus

“…is a wild basidiomycete, native to Brazil, with pantropical (tropical regions of Africa, Asia and America) and neotropical (Central America, southern Florida, Caribbean islands and South America) distribution, frequently found in decomposing tree trunks (SILVA;GIBERTONI, 2006). This fungus grows in several substrates and is utilized in bioremediation (BALLAMINUT;MATHEUS, 2007), product discoloring in the textile industry (SANCHEZ-LOPEZ et al, 2008;NIEBISCH et al, 2010;ALMEIDA et al, 2018;MARIM et al, 2018) and presents a powerful arsenal of enzymes dedicated to the breakdown and consumption of lignocellulose (VALLE et al, 2014;CAMBRI et al, 2016;CONCEIÇÃO et al, 2017;SANTANA et al, 2018). Moreover, it produces a fungal secondary metabolite panepoxydone that has been described as an inhibitor of NF-κB activation, and it is a regulator of the inflammatory and immune response WISSER;ANKE, 2007;WASSER, 2010;WASSER, 2012;WASSER, 2017).…”