Mycelial biomass cultivation of Lentinus crinitus

Abstract

Lentinus crinitus is a medicinal basidiomycete, little studied regarding the basic cultivation conditions, which is used in bioremediation and consumed by native Indians from the Brazilian Amazon. Also, it produces a fungal secondary metabolite panepoxydone that has been described as an essential regulator of the inflammatory and immune response. This study aimed to evaluate basic conditions of temperature, pH, and nitrogen concentration and source in the cultivation of L. crinitus mycelial biomass. In order to evaluate fungal growth temperature, 2% malt extract agar (MEA) medium, pH 5.5, was utilized from 19 to 40 °C. For pH, MEA had pH adjusted from 2 to 11 and cultivated at 28 °C. Urea or soybean meal was added to MEA to obtain final concentration from 0.5 and 16 g/L of nitrogen, pH of 5.5, cultivated at 28 °C. The best temperature growth varies from 31 to 34 ºC and the optimal one is 32.7º C, and the best pH ranges from 4.5 to 6.5 and the optimal one is 6.1. Protein or non-protein nitrogen concentration is inversely proportional to the mycelial biomass growth. Nitrogen concentrations of 2.0 g/L soybean meal and urea inhibit mycelial biomass growth in 11% and 12%, respectively, but high concentrations of 16.0 g/L nitrogen inhibit the growth in 46% and 95%, respectively. The fungus is robust and grows under extreme conditions of temperature and pH, but smaller adaptation with increasing nitrogen concentrations in the cultivation medium, mainly non-protein nitrogen.

Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp

“…56,57 This oligosaccharide may also be related to L. crinitus enzymatic activity 29 and mycelial biomass production, mainly at extreme temperatures (19 to 40 °C) and pH (2.5 to 10) conditions. 58 Edible mushroom fat contents were reported from 1.1 to 8.3 g per 100 g. 50 For L. crinitus basidiocarp cultivated in a substrate based on Theobroma grandiflorum exocarp, the fat content ranged from 3.3 to 4.5 g per 100 g (ref. 16) and was 1.5 g per 100 g for wild L. crinitus basidiocarp collected in the Brazilian Cerrado.…”

Lentinus crinitus basidiocarp stipe and pileus: chemical composition, cytotoxicity and antioxidant activity

“…Trehalose migrates from mycelia to basidiocarps, and protects the cells against stresses such as desiccation, temperature, and oxygen pressures [60,61]. This protection mechanism seems to be related to malondialdehyde inhibition, a free radical naturally produced by lipid peroxidation with mutagenic and carcinogenic activities, and it could explain the capacity of L. crinitus enzymatic production [19] and mycelial biomass growth [62] under extreme conditions of temperature and pH.…”

Food and nutritional potential of two mushrooms native species to the Brazilian savanna (Cerrado)

“…Few works have been found in the literature on substrates for cultivating native Cerrado species so far. An exception is a recent work on L. crinitus, in which the authors highlight the capacity of this species to grow in environments and situations considered extreme for fungal development (Colla et al, 2020) Next, we present a dichotomous key based on external morphology which will facilitate the identification of the macrofungi analyzed in this work in the field, constituting native species of the Brazilian Cerrado with food potential. Figure 1 presents the main characteristics mentioned in the key which complement recognizing the species in the field.…”