Autochthonous probiotic as growth promoter and immunomodulator forAstyanax bimaculatuscultured in water recirculation system

Abstract

This work aimed to evaluate the probiotic Lactobacillus spp. in the microbiota of Astyanax bimaculatus reared in a water recirculation system. The experiment was randomized, using 800 postlarvae distributed into eight polyethylene containers, separated into two treatments, in quadruplicate: supplementation with Lactobacillus spp. (Probiotic) and no supplementation (Control). After 90 days of culture, 13 fish per container were anaesthetized. Three of these underwent microbiological evaluation, and haematological analyses were carried out on five. Immunological assessment was performed on another five fish. Zootechnical parameters were evaluated for all animals. In the microbiological evaluation, the probiotic group presented higher counts of lactic acid bacteria, but lower counts of Vibrio spp., Pseudomonas spp., and Staphylococcus spp., when compared with control. After haematological analysis, the supplemented group presented low haematimetric indices of Mean Corpuscular Haemoglobin Concentration and Mean Corpuscular Haemoglobin (MCHC and MCH, respectively) and higher amounts of total leucocytes, thrombocytes and circulating monocytes when compared with control. The immunological profile did not differ between treatments. In the zootechnical performance, probiotic group presented higher productivity, survival and apparent food efficiency when compared with control. Therefore, when Lactobacillus spp. was supplemented in the diet of A. bimaculatus, immunocompetence and the zootechnical performance of animals increased. K E Y W O R D Shaematology, immunology, Lactobacillus spp., microbiology, yellow tail lambari

Sex disparities determined by biometric and hematological measurements in Astyanax bimaculatus

“…Fish hematology is an important tool for identifying changes in homeostasis, as well as the appearance of diseases and standardizing ideal reared conditions (Fazio, 2019). The hematological parameters of male and female A. bimaculatus did not show significant differences, except in HCM (Table 3), while other studies reported values close to those found in this experiment related to the number of erythrocytes, hematocrit, hemoglobin, glucose, MCV, MCHC and MCH in A. bimaculatus submitted to environmental conditions similar to this study (Jatobá et al, 2018;Moraes et al, 2018).…”

“…The experimental design was completely randomized, and four hundred fish from the same spawning, with a mean weight 0.27 g, were distributed into four experimental units (800 L polyethylene tanks) of 100 fish each and equipped with a recirculation aquaculture system (RAS) and biological filter ( Figure 1). The fish were fed four times a day with 3% of their biomass, according to (Moraes et al, 2018). During the experimental period, dissolved oxygen (3.15±1.52 mg.L-1) and temperature (21.78±2.59°C morning; 23.51±2.37°C afternoon), were measured twice a day.…”

Effects of anEnterococcus faecium‐based probiotic on growth performance and health of Pirarucu,Arapaima gigas

“…In this context, reports are available indicating the use of probiotics to reduce disease outbreaks by enhancing immunological system (Dias et al, ; Soltani, Ghosh, et al, ; Soltani, Lymbery, Song, & Hosseini‐Shekarabi, ). The use of probiotics is a strategy to increase production of fish, modulating the intestinal microbiota (Mouriño et al, ; Standen et al, ), improving the immune response (Giri, Sukumaran, & Oviya, ; Jatobá Moraes, Rodrigues, Vieira, & Pereira, ; Moraes et al, ; Mouriño et al, ) and improving growth performance (Dias et al, ; Giri et al, ; Hamdan, El‐Sayed, & Mahmoud, ), during diverse stages of growth (Hamdan et al, ; Jha, Bhujel, & Anal, ; Nakandakare et al, ; Ridha & Azad, ).…”

“…Lactic acid bacteria have shown to be promising probiotics with positive results in various aquatic species including Dicentrarchus labrax (Piccolo et al, ), Oreochromis niloticus (Jatobá et al, , ), Labeo rohita (Giri et al, ) and Astyanax bimaculatus (Moraes et al, ). The positive effect is due to their capacity to colonize the gastrointestinal tract, modifying the microbiota of the intestine and inhibiting the growth of other microorganisms, and improving the immune system (Giri et al, ; Jatobá et al, ; Mouriño et al, ; Yamashita et al, ).…”

Probiotics in Animal Husbandry: Applicability and Associated Risk Factors

“…Moreover, a diet of Astyanax bimaculatus supplemented with Lactobacillus spp. resulted in increased amounts of leucocytes in the circulatory system, thus conferring greater resistance to gut pathogens and, consequently, higher survival rates, in addition to improved feed efficiency [66]. In another study conducted by Vase-Khavari et al [67], poultry bird feed supplemented with probiotics (superzist) revealed significant influences on the growth performance of the broilers, with laudable responses on total cholesterol, triglyceride levels and immunological parameters, and a notable reduction in colon pathogens.…”