Autochthonous canine babesiosis in The Netherlands

Matjila, Paul Tshepo; Nijhof, Ard M.; Taoufik, Amar; Houwers, D.J.; Teske, Erik; Penzhorn, Barend Louis; Lange, T. de; Jongejan, Frans

doi:10.1016/j.vetpar.2005.04.020

Cited by 68 publications

(43 citation statements)

References 15 publications

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“…Detection of Babesia infection in ticks has been widely used in epidemiological studies in various countries , Matjila et al 2005, M'Ghirbi & Bouattour 2008, Majláthová et al 2011. However, in Brazil, few studies have been performed and little is known about the aspects of infection with Babesia spp.…”

Section: Discussionmentioning

confidence: 99%

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

et al. 2015

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Abstract:This study aimed to report the prevalence of Babesia canis vogeli in dogs and ticks in the urban and rural areas of Petrolina, Pernambuco. Serum and peripheral blood samples of 404 dogs were tested by indirect immunofluorescence assay (IFA) and by blood smears, respectively. The presence of tick infestation was evaluated, and some specimens were submitted to DNA amplification by polymerase chain reaction (PCR). The presence of antibodies anti-B. canis vogeli was determinate in 57.9% (234/404) of dogs. The direct detection of Babesia spp was obtained in 0.5% (2/404) dogs by visualization of intraerythrocytic forms. Infestation by Rhipicephalus sanguineus sensu lato was observed in 54.5% (220/404) of dogs in both urban and rural areas. DNA of Babesia canis vogeli were obtained by PCR in 6% individual (3/50) and 8.7% of pool of ticks (7/80). The risk factors for the presence of anti-B. canis vogeli antibodies, as determined through the application of logistic regression models (P<0.05), were the following: medium breed size variables (P<0.001); contact with areas of forest (P=0.021); and access on the street (P=0.046). This study describes, for the first time, the confirmation of infection of B. canis vogeli in dogs and ticks in the semiarid region of Pernambuco, Brazil.

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Section: Discussionmentioning

confidence: 99%

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

et al. 2015

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“…The reaction was then followed by 40 cycles of denaturation at 94 8C for 30 s, annealing at 57 8C for 30 s and extension at 72 8C for 30 s. PCR-amplified products were tested with the RLB, as previously described (Matjila et al, 2004). An additional plasmid control was used as an internal positive control to ensure that all Babesia species-specific probes were correctly bound to the RLB membrane and that they were functional (Matjila et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

Detection of a Theileria species in dogs in South Africa

Matjila

Leisewitz

Oosthuizen

et al. 2008

Veterinary Parasitology

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A Theileria species was detected by PCR in blood samples collected from dogs in the Pietermaritzburg area and was also found in dogs presented at the Outpatients Clinic of the Onderstepoort Veterinary Academic Hospital (OVAH), in the Pretoria area, South Africa. In the Pietermaritzburg area, 79 of the 192 samples were positive, while 3 out of 1137 of the Onderstepoort samples were positive. Three positive samples from Pietermaritzburg were co-infected with Ehrlichia canis. PCR positive samples were further analysed by the Reverse Line Blot (RLB) and sequence analysis. Phylogenetic analysis of the 18S rRNA full-length gene sequences of one sample (VT12) from Pietermaritzburg and two samples from OVAH (BC281 and BC295) revealed a close relationship with sequences of Theileria species (sable). Clinical signs of the dogs that were examined at Pietermaritzburg and OVAH included an immune-mediated condition with severe thrombocytopenia. These findings identify a Theileria sp. in dogs for the first time in South Africa and add yet another microorganism to the growing list of haemoprotozoan parasites infecting dogs worldwide. The clinical significance of this infection in dogs is poorly resolved. #

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“…Köpeklerde B. c. canis'in neden olduğu babesiosis özel-llikle Orta Avrupa ve Rusya'da yaygın olarak görülmekte olup, son yıllarda kuzeye doğru bir yayılış göstererek Norveç ve Hollanda gibi ülkelerde de rapor edilmeye başlanmıştır [1,2,16,17] . Etken D. reticulatus tarafından nakledilmekte olup, hastalık bu kenenin yayılış alanları ile ilişkilidir [1,2] .…”

Section: Tartişma Ve Sonuçunclassified