Detection of a Theileria species in dogs in South Africa

Matjila, Paul Tshepo; Leisewitz, Andrew L.; Oosthuizen, Marinda C.; Jongejan, Frans; Penzhorn, Barend Louis

doi:10.1016/j.vetpar.2008.06.025

Cited by 86 publications

(67 citation statements)

References 35 publications

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“…A newly identified species (Theileria sp. dog) was detected in three specimens (Matjila et al, 2008). Mixed infections were also detected: eight specimens were co-infected with B. rossi and Ehrlichia canis; seven specimens were co-infected with Babesia vogeli and E. canis; one specimen was co-infected with B. rossi and B. vogeli and one specimen had a triple infection of B. rossi, B. vogeli and E. canis.…”

Section: Resultsmentioning

confidence: 91%

“…We detected only one specimen co-infected with B. rossi and B. vogeli and we also detected only one specimen co-infected with B. rossi, B. vogeli and E. canis. Of interest was the detection of a Theileria species from three specimens collected at OVAH, as we are not aware of any Theileria species ever having been isolated from dogs in South Africa (Matjila et al, 2008). B. rossi was the common species detected from all sampled areas.…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa

Matjila¹,

Leisewitz²,

Jongejan³

et al. 2008

Veterinary Parasitology

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A total of 1138 blood specimens were collected over a 6-year period (2000)(2001)(2002)(2003)(2004)(2005)(2006) from domestic dogs in South Africa. Specimens from domestic dogs were obtained from the Onderstepoort Veterinary Academic Hospital (OVAH) in Pretoria, the Society for the Prevention of Cruelty to Animals (Johannesburg, Durban, East London and Bloemfontein) and private practices from four provinces (Gauteng, Mpumalanga, KwaZulu-Natal and Western Cape). All specimens were screened for Babesia, Theileria, Hepatozoon and Ehrlichia/Anaplasma species using PCR and Reverse Line Blot (RLB) assays. On RLB, 560/1137 domestic dog-specimens were positive for one or more parasites. Of the positive domestic dog-specimens, 420 (75%) were infected with Babesia rossi; 82 (15%) dogs were infected with Theileria sp. (dog); 18 (3%) dogs were infected with Babesia vogeli; 14 (3%) specimens were infected with Ehrlichia canis. Mixed infections were also found: B. rossi and E. canis were detected in 12 (2%) specimens; B. vogeli and E. canis occurred in 7 (1%) specimens; Theileria sp. (dog) and E. canis in 3 (0.5%) specimens; B. rossi and B. vogeli in one specimen. B. rossi, B. vogeli and E. canis occurred simultaneously in one dog. There was also one incidental finding of a dog positive for Trypanosoma congolense. The results indicate that a wide range of tick-borne pathogens are circulating in the canine populations in South Africa. #

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Section: Resultsmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa

Matjila¹,

Leisewitz²,

Jongejan³

et al. 2008

Veterinary Parasitology

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“…(MATJILA et al, 2008) and a yet undescribed species of large Babesia identified in North Carolina, USA (BIRKENHEUER et al, 2004;LEHTINEN et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, other small piroplasms are known to infect dogs including Theileria annae (ZAHLER et al, 2000), B. conradae (CONRAD et al, 1991;CONRAD, 2006), T. annulata (CRIADO et al, 2006), T. equi (CRIADO-FORNELIO et al, 2003) and Theileria sp. (MATJILA et al, 2008). Rangelia vitalii is an intracellular parasite belonging to the phylum Apicomplexa and order Piroplasmida (LORETTI;BARROS, 2004).…”

Section: Detection and Molecular Characterization Of Piroplasms Specimentioning

confidence: 99%

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Lemos

Cerqueira

Toma

et al. 2012

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.Keywords: Babesia vogeli, Rangelia vitalii, piroplasms, dogs, PCR. ResumoRangelia vitalii é um protozoário que infecta cães e foi descrito nas regiões Sul e Sudeste do Brasil. R. vitalii é filogeneticamente próxima à Babesia spp., mas dados deste misterioso parasito ainda são escassos. O objetivo deste trabalho foi detectar a presença de piroplasmas em cães naturalmente infectados no estado do Rio de Janeiro, através da amplificação do gene 18S rRNA pela PCR, clivagem com enzimas de restrição (RFLP) e caracterização genética através do sequenciamento. De 103 cães, sete (6,8%) foram positivos para Babesia spp. pela PCR. Os produtos amplificados foram digeridos por enzimas de restrição para a diferenciação das espécies de Babesia e uma amostra foi identificada como Babesia vogeli. O padrão de amplificação observado nas outras seis amostras não correspondeu ao padrão descrito para babesias que infectam cães. O sequenciamento das seis amostras confirmou ser uma espécie geneticamente idêntica a R. vitalii apresentando grande homologia (99-100%) com a sequência do sul do Brasil. Este estudo confirma a presença de Babesia vogeli e Rangelia vitalii infectando cães em Teresópolis, Rio de Janeiro, Brasil.

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“…In South Africa, Matjila et al (2008) examined some dogs with an immune mediated condition with severe thrombocytopenia and detected Theileria species in one sample from Pietermaritzburg and samples from Onderstepoort Veterinary Academic Hospital (OVAH) by PCR in blood samples. Smear examination of three OVAH sample did not show any piroplasms, but may have been under the detection limits for routine light microscopy as often encountered in T. annae infections (Garcia 2006).…”

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confidence: 99%

Evidence of new pathogenic Theileria species in dogs

Dixit

Dixit²,

Varshney³

2010

J Parasit Dis

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Babesiosis, caused by tick-borne haematozoan organisms of the genus Babesia, is a parasitic disease of domestic and wild mammals. Canine Babesidae have historically been classified as ''large Babesia'' (Babesia canis) and ''small Babesia'' (Babesia gibsoni) based on the size of their intraerythrocytic forms. Recent publications, however, suggest that the diversity of piroplasm species infecting dogs might be greater than previously appreciated. Sequencing and phylogenetic analysis of the ssrRNA gene has revealed that canine piroplasms are found in three clades, 'true' Babesia sp. (B. canis and B. gibsoni), Theileria annae and Theileria-like group (B. conradae). This newly recognised piroplasm T. annae appears to be hyperendemic in northwest Spain. The vector for this emergent canine infection remains undescribed, although the Ixodes hexagonus is suspected based on their presence upon the dogs in NW Spain and the relative absence of others. These observations have introduced a major change in the approach to the epidemiology of babesiosis in dogs. More clinical samples and data will need to be collected and analyzed to understand the importance of Theileria species in dogs.

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Detection of a Theileria species in dogs in South Africa

Cited by 86 publications

References 35 publications

Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa

Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Evidence of new pathogenic Theileria species in dogs

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