Authentication of  Myristica fragrans  Houtt. using DNA barcoding

Swetha, V. P.; Parvathy, V. A.; Sheeja, T. E.; Sasikumar, B.

doi:10.1016/j.foodcont.2016.10.004

Cited by 29 publications

(12 citation statements)

References 38 publications

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“…Similar substitution were reported for other species, such as Sida cordifolia L. (76%) (Vassou et al, 2015), Cinnamomum verum J.Presl (70%) (Swetha et al, 2014), Myristica fragrans Houtt. (60%) (Swetha et al, 2017), Senna auriculata (L.) Roxb. (50%) (Seethapathy et al, 2015), Senna tora (L.) Roxb.…”

Section: Introductionmentioning

confidence: 99%

DNA Metabarcoding Authentication of Ayurvedic Herbal Products on the European Market Raises Concerns of Quality and Fidelity

et al. 2019

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Ayurveda is one of the oldest systems of medicine in the world, but the growing commercial interest in Ayurveda based products has increased the incentive for adulteration and substitution within this herbal market. Fraudulent practices such as the use of undeclared fillers and use of other species of inferior quality is driven both by the increased as well as insufficient supply capacity of especially wild plant species. Developing novel strategies to exhaustively assess and monitor both the quality of raw materials and final marketed herbal products is a challenge in herbal pharmacovigilance. Seventy-nine Ayurvedic herbal products sold as tablets, capsules, powders, and extracts were randomly purchased via e-commerce and pharmacies across Europe, and DNA metabarcoding was used to assess the ability of this method to authenticate these products. Our analysis reveals that only two out of 12 single ingredient products contained only one species as labeled, eight out of 27 multiple ingredient products contained none of the species listed on the label, and the remaining 19 products contained 1 to 5 of the species listed on the label along with many other species not specified on the label. The fidelity for single ingredient products was 67%, the overall ingredient fidelity for multi ingredient products was 21%, and for all products 24%. The low level of fidelity raises concerns about the reliability of the products, and detection of threatened species raises further concerns about illegal plant trade. The study highlights the necessity for quality control of the marketed herbal products and shows that DNA metabarcoding is an effective analytical approach to authenticate complex multi ingredient herbal products. However, effort needs to be done to standardize the protocols for DNA metabarcoding before this approach can be implemented as routine analytical approaches for plant identification, and approved for use in regulated procedures.

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Section: Introductionmentioning

confidence: 99%

DNA Metabarcoding Authentication of Ayurvedic Herbal Products on the European Market Raises Concerns of Quality and Fidelity

et al. 2019

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“…Tree 18 65 18 No M: Fruits are eaten to cure male impotent and also to increase fertility 1,8,12,18,24,30,35 (Lansky et al, 2008 Fruits are used as antidiabetic 3,6,12,15,20,32,35,38,39 (Swetha et al, 2017) Phoenix loureiroi Kunth; Arecaceae; Cl 809…”

Section: Author Contributionsmentioning

confidence: 99%

Ethnobotany of dioecious species: Traditional knowledge on dioecious plants in India

Seethapathy

Ravikumar

Paulsen

et al. 2018

Journal of Ethnopharmacology

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A knowledge gap exists in ethnobotanical and ethnopharmacological literature on traditional knowledge of dioecious plants. From this explorative study it is evident that people have traditional knowledge on plant gender and preferential usages towards one gender. Based on this, we propose that researchers conducting ethnobotanical and ethnopharmacological studies should consider documenting traditional knowledge on sexual systems of plants, and test the existence of gender specific usages in their conceptual framework and hypothesis testing. Incorporating such concepts could provide new dimensions of scientific knowledge with potential implications to conservation biology, chemical ecology, ethnoecology and drug discovery.

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“…Neolignans and phenolic compounds, present in the methanolic extract of mace, showed inhibitory effects on mediators in the inflammatory pathway and antiproliferative effects [18]. DNA barcoding of M. fragrans (mace) was investigated for the identification of M. malabarica adulteration [19]. e safety and efficacy of mace powder combined with pelvic floor muscle training were assessed in a placebo-controlled trial in patients with urinary incontinence.…”

Section: Introductionmentioning

confidence: 99%

Anti-Helicobacter pylori, Anti-Inflammatory, Cytotoxic, and Antioxidant Activities of Mace Extracts from Myristica fragrans

Suthisamphat

Dechayont

Phuaklee

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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e aril (mace) of Myristica fragrans, known as Dok-Chan, is a spice that has long been used for treating stomach discomfort, peptic ulcer, and nausea. It is an ingredient in many remedies in ai traditional medicine, e.g., Ya-Hom-ep-Bha-Jit, Ya-Hom-Nao-Wa-Kot, and Ya-at-Bun-Job, which are used to treat dyspepsia and other gastrointestinal tract symptoms. e aqueous and ethanolic extracts of mace were used for all tests. Anti-H. pylori activities were determined by the disc diffusion method and agar dilution. Anti-inflammatory activity was determined by the LPS-induced nitric oxide (NO) inhibition in a RAW264.7 cell line, and cytotoxicity was determined against gastric cancer cell lines (Kato III) using the sulphorhodamine B (SRB) assay. e DPPH radical scavenging and ABTS radical cation decolorization assays were used to determine the antioxidant activities. e result found that the ethanolic extract of mace exhibited antimicrobial activity against H. pylori ATCC 43504 and six clinical strains with MIC values of 125-250 μg/ml. e aqueous extract MICs against H. pylori ATCC reference strain and six clinical strains were 500 μg/ml compared with 0.5 μg/ml for the positive control, clarithromycin. e inhibitory effect of LPS-induced NO release and cytotoxic activity of the ethanolic extract had IC 50 values of 82.19 μg/ml and 26.06 μg/ml, respectively, and the EC 50 values for the DPPH and ABTS antioxidant assays were 13.41 μg/ml and 12.44 μg/ml, respectively. e mace extract also had anticancer properties. In conclusion, the ethanolic mace extract had anti-H. pylori, anti-inflammatory, antioxidant, and anticancer activities. ese data support further preclinical and clinical investigation to see if the mace extract could have a role in treating patients with dyspepsia, peptic ulcers, and possibly gastric cancer.

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Authentication of Myristica fragrans Houtt. using DNA barcoding

Cited by 29 publications

References 38 publications

DNA Metabarcoding Authentication of Ayurvedic Herbal Products on the European Market Raises Concerns of Quality and Fidelity

DNA Metabarcoding Authentication of Ayurvedic Herbal Products on the European Market Raises Concerns of Quality and Fidelity

Ethnobotany of dioecious species: Traditional knowledge on dioecious plants in India

Anti-Helicobacter pylori, Anti-Inflammatory, Cytotoxic, and Antioxidant Activities of Mace Extracts from Myristica fragrans

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