Anti-Helicobacter pylori, Anti-Inflammatory, Cytotoxic, and Antioxidant Activities of Mace Extracts from Myristica fragrans

Abstract

e aril (mace) of Myristica fragrans, known as Dok-Chan, is a spice that has long been used for treating stomach discomfort, peptic ulcer, and nausea. It is an ingredient in many remedies in ai traditional medicine, e.g., Ya-Hom-ep-Bha-Jit, Ya-Hom-Nao-Wa-Kot, and Ya-at-Bun-Job, which are used to treat dyspepsia and other gastrointestinal tract symptoms. e aqueous and ethanolic extracts of mace were used for all tests. Anti-H. pylori activities were determined by the disc diffusion method and agar dilution. Anti-inflammatory activity was determined by the LPS-induced nitric oxide (NO) inhibition in a RAW264.7 cell line, and cytotoxicity was determined against gastric cancer cell lines (Kato III) using the sulphorhodamine B (SRB) assay. e DPPH radical scavenging and ABTS radical cation decolorization assays were used to determine the antioxidant activities. e result found that the ethanolic extract of mace exhibited antimicrobial activity against H. pylori ATCC 43504 and six clinical strains with MIC values of 125-250 μg/ml. e aqueous extract MICs against H. pylori ATCC reference strain and six clinical strains were 500 μg/ml compared with 0.5 μg/ml for the positive control, clarithromycin. e inhibitory effect of LPS-induced NO release and cytotoxic activity of the ethanolic extract had IC 50 values of 82.19 μg/ml and 26.06 μg/ml, respectively, and the EC 50 values for the DPPH and ABTS antioxidant assays were 13.41 μg/ml and 12.44 μg/ml, respectively. e mace extract also had anticancer properties. In conclusion, the ethanolic mace extract had anti-H. pylori, anti-inflammatory, antioxidant, and anticancer activities. ese data support further preclinical and clinical investigation to see if the mace extract could have a role in treating patients with dyspepsia, peptic ulcers, and possibly gastric cancer.

Antifungal, Antibacterial, and Cytotoxic Activities of Silver Nanoparticles Synthesized from Aqueous Extracts of Mace-Arils of Myristica fragrans

“…In many parts of Asia, mace is referred to as javitri or bisbasah. In Unani medicine, Ayurvedic, and Chinese traditional medicine, mace is commonly used for treating peptic ulcers, flatulence, stomach discomfort, gastrointestinal symptoms, and anxiety [36][37][38]. Very few reports have shown the antibacterial activity and anti-inflammatory properties of mace extracts.…”

HR-LC-MS based profiling of phytochemicals from methanol extracts of leaves and bark of Myristica dactyloides Gaertn. from Western Ghats of Karnataka, India

“…In addition to the above phytochemical constituents, the presence of compounds Mal C and Mal B, which are present in most of the members of genus Myristica potentially proves its consideration in developing a chemo-taxonomical library for the identification of this genus. Mal C is one among the extensively explored chemical content predominantly present in genus Myristica, known for its possible therapeutic potential for treating cancer and inflammatory disorders [51,52], Alzheimer's disease [53], and infectious diseases [54]. The present study also highlights the…”

Antibacterial, anti-inflammatory and antioxidant activities of Mahanintangtong and its constituent herbs, a formula used in Thai traditional medicine for treating pharyngitis

“…Following 4 h of incubation, the medium was removed and 100 μL of isopropanol containing 0.04 M HCl was added to dissolve the formazan production in the cells. The OD of formazan solution was measured with a microplate reader at 570 nm [ 20 ]. The test simples were considered to be cytotoxic when the OD of the sample-treated group was less than 70% of that in the control (vehicle-treated) group.…”