Aumento do volume do baço em modelo experimental de hipertensão portal pré-hepática em ratos

Silva, Aline Riquena da; Kriguer-Júnior, Ricardo José; Serigiolle, Leonardo Carvalho; Gomes, Helbert Minuncio Pereira; Rodrigues, Daren Athiê Boy; Leme, Pedro Luiz Squilacci

doi:10.1590/s0102-67202013000300010

Cited by 10 publications

(10 citation statements)

References 20 publications

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“…The spleen is the only solid organ of the portal venous system. In patients with PHT, portal venous blood returns flow and the splenic functions as an auxiliary capacitance system which compensates for the increase in portal pressure that may cause splenomegaly [ 13 ]. The splenic angiogenesis can regulate the portal systemic collateral circulation and increase splenic blood flow, which is a pathophysiologic hallmark of the spleen [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Long-term efficacy of subtotal splenectomy due to portal hypertension in cirrhotic patients

et al. 2015

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BackgroundPortal hypertension (PHT) requires invasive measures to prevent rupture and bleeding of esophagogastric varices; however, the long-term results of subtotal splenectomy plus fixation of the retrosternal omentum majus (SSFROM) have not been reported. Specifically, the advantages and disadvantages of surgery that preserves the spleen and the long-term hematologic effects have not been described.Study designOur studies relating to SSFROM commenced in February 1999. As of April 2014 we have performed 256 subtotal splenectomies The records of 65 patients with PHT who underwent SSFROM were reviewed retrospectively.ResultsFour patients died within 4 years of surgery, with a 4-year survival rate of 94 %; the 11-year survival rate was 60 %. Eleven patients (17 %) had re-bleeding from esophagogastric varices. The white blood cell and platelet counts were higher 6 and 11 years post-operatively compared with pre-operative values (P < 0.01). Portal venous diameter, portal venous flow volume, splenic artery flow volume, as well as splenic length, thickness, and average cross-sectional areas were shown to be significantly constricted or decreased (P < 0.01). The proportion of serum CD3+ T cells, CD4+ T cells, and CD8+ T cells was increased (P < 0.01), while the serum levels of macrophage colony-stimulating factor and granulocyte-macrophage colony-stimulating factor were significantly decreased (P < 0.01). There was no significant change in the serum levels of IgA, IgM, IgG, and Tuftsin (P > 0.05). DSA demonstrated that 15 cases formed collateral circulations between the portal vein and superior vena cava.ConclusionSSFROM provide long-term hemostasis for esophagogastric variceal bleeding in PHT and corrected hypersplenism. SSFROM is an effective treatment for patients with PHT in whom long-term survival is expected.

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Section: Discussionmentioning

confidence: 99%

Long-term efficacy of subtotal splenectomy due to portal hypertension in cirrhotic patients

et al. 2015

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“…Riquena da Silva et al 22 studied the spleen volume of rats in an experimental model of pre-hepatic portal hypertension, using the same methodology to calculate the volume of the spleen, which was evaluated in live animals. During the first operation performed, they found an average initial volume of 0.5417 cm 3 .…”

Section: Discussionmentioning

confidence: 99%

Volume calculation of rats' organs and its application in the validation of the volume relation between the abdominal cavity and the hernial sac in incisional hernias with "loss of abdominal domain"

Araújo¹,

Serigiolle²,

Gomes³

et al. 2014

ABCD, arq. bras. cir. dig.

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BackgroundThe calculation of the volume ratio between the hernia sac and the abdominal cavity of incisional hernias is based on tomographic sections as well as the mathematical formula of the volume of the ellipsoid, which allows determining whether this is a giant hernia or there is a "loss of domain". As the images used are not exact geometric figures, the study of the volume of two solid organs of Wistar rats was performed to validate these calculations.AimTo correlate two methods for determining the volume of the kidney and spleen of rats, comparing a direct method of observation of the volume with the mathematical calculation of this value.MethodsThe volume of left kidney, geometrically more regular, and spleen, with its peculiar shape, of ten animals was established in cubic centimeters after complete immersion in water with the aid of a beaker graduated in millimeters. These values were compared with those obtained by calculating the same volume with a specific mathematical formula: V = 4/3 × π × (r1 x r2 x r3). Data were compared and statistically analyzed by Student's t test. RESULTS: Although the average volume obtained was higher through the direct method (1.13 cm3 for the left kidney and 0.71 cm3 for the spleen) than the values calculated with the mathematical formula (0.81 cm3 and 0.54 cm3), there were no statistically significant differences between the values found for the two organs (p>0.05).ConclusionThere was adequate correlation between the direct calculation of the volume of the kidney and spleen with the result of mathematical calculation of these values in the animals' studies.

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“…This experiment was conducted at the Laboratory of Advanced Surgical Skills at Universidade Nove de Julho, São Paulo, SP, Brazil after being approved by the Ethics Committee on Animal Use of the University (protocol 0041/2012). The care with the preparation of the animals, anesthesia, postoperative analgesia and general care at the vivarium were strictly supervised in the institution and followed specific protocols 24 . As the mortality of animals was significant, in respect to ethical principles of animal experimentation, the experimental model was discontinued without the study of biochemical parameters such as the dosage of bicarbonate, the evaluation of pH, blood gases and histological study of the liver were performed.…”

Section: Methodsmentioning

confidence: 99%

Unfeasible experimental model of normothermic hepatic ischemia and reperfusion in rats using the Pringle maneuver

Gomes¹,

Serigiolle²,

Rodrigues³

et al. 2014

ABCD, arq. bras. cir. dig.

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BackgroundThe negative result of a research does not always indicate failure, and when the data do not permit a proper conclusion, or are contrary to the initial project, should not simply be discarded and archived.AimTo report failure after performing experimental model of liver ischemia and reperfusion normothermic, continuous or intermittent, in small animals aiming at the study of biochemical and histological parameters after postoperative recovery.MethodsFifteen Wistar rats were divided into three groups of five animals each; all underwent surgery, the abdomen was sutured after the proposed procedures for each group and the animals were observed for 6 h or until they died, and then were reoperated. In Group 1, control (sham-operated): dissection of the hepatic hilum was performed; in Group 2: clamping of the hepatic hilum for 30 m; in Group 3: clamping of the hepatic hilum for 15 m, reperfusion for 5 m and another 15 m of clamping. Data from Groups 2 and 3 were compared with Student's t test.ResultsAll animals of Group 1 survived for 6 h. Two animals in Group 2 died before the 6 h needed to validate the experiment; two did not recover from anesthesia and one survived until the end. In Group 3, four animals died before the 6 h established and one of them survived the required time. Only one animal in Group 2 and one in Group 3 survived and were able to accomplish the study. There was no statistical significance when the results of Groups 2 and 3 were compared (p>0.05).ConclusionThe death of six animals before the necessary period of observation turned the initial proposal of the experiment unfeasible.

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Aumento do volume do baço em modelo experimental de hipertensão portal pré-hepática em ratos

Cited by 10 publications

References 20 publications

Long-term efficacy of subtotal splenectomy due to portal hypertension in cirrhotic patients

Long-term efficacy of subtotal splenectomy due to portal hypertension in cirrhotic patients

Volume calculation of rats' organs and its application in the validation of the volume relation between the abdominal cavity and the hernial sac in incisional hernias with "loss of abdominal domain"

Unfeasible experimental model of normothermic hepatic ischemia and reperfusion in rats using the Pringle maneuver

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