Augmentation of bone healing of nonunion fracture using stem cell based tissue engineering in a dog: a case report

Abstract

ABSTRACT:A 4-year-old, intact male crossbreed dog, weighing 27 kg, was referred for the treatment of a nonunion fracture. The radiographs revealed displacement of the radius and ulna bone fracture fragment and a sclerotic fracture end of the radius. Autologous adipose derived stem cells (ADSCs) were isolated and expanded ex vivo in a culture. The ADSCs (3.2 × 107 cells) were seeded on a composition scaffold made from hydroxyapatite (HA) and chitosan (CH) fibers. The seeded scaffold with ADSCs was placed on the fracture site and the bone fracture was stabilized. A sample of seeded scaffold with ADSCs was taken to evaluate the extent of cell attachment and morphology on the scaffold using scanning electron microscopy (SEM). SEM showed that ADSCs adhered to the scaffold well and many bone nodules formed from the bone matrix secreted by ADSCs. Three months after surgery, the nonunion had successfully healed with no complications. The application of a composition scaffold of HA and CH containing ADSCs can be used to treat a nonunion fracture by augmenting bone healing and may decrease the risk of surgical failure of nonunion fractures.

Stem Cells and Biological Approaches to Treatment of Wrist Problems

“…Lee et al treated a case of canine nonunion fracture with autologous adipose-derived MSCs seeded on a hydroxyapatite/chitosan composite scaffold. 26 Three months after surgery, the nonunion successfully healed with no complications.…”

Therapeutic potential of stem cells in veterinary practice

“…In veterinary sciences, stem cells are mainly used for chitosan scaffold [87]. In another study allogenic the treatment in canines and equines whereas research transplantation of canine MSCs proved worthy for is still going on in other farm animals.…”

Evaluation of Canine Adipose-derived Stem Cells in a Healthy Mice Subcutaneous Model

“…The canine adipose-derived stem cells (cASCs) have recently been reported in several studies in Regenerative Medicine with promising results. Concerning clinical studies, cASCs have been used in combination with scaffolds based on hydroxyapatite and chitosan (30:70 wt%) for the treatment of radius and ulna nonunion fracture in dog [9], or in the treatment of dogs with lameness associated with osteoarthritis of the coxofemoral [10] and humeroradial [11,12] joints. Although without effective clinical improvement, cASCs have also been tested in the treatment of canine atopic dermatitis [13].…”