Evaluation of Canine Adipose-derived Stem Cells in a Healthy Mice Subcutaneous Model

Requicha, João Filipe; Carvalho, Pedro P.; Pires, Maria dos Anjos; Dias, Isabel

doi:10.4172/2157-7633.1000358

Cited by 1 publication

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to murine MSCs, the transformed canine MSCs did not form tumors in athymic mice, both subcutaneously and intratibially. It might be that canine cells have difficulty engrafting in a mouse microenvironment, however, previously published studies reported the successful growth of (adipose-derived) MSCs and osteosarcoma cell lines from canine origin in immune deficient mice, both subcutaneously and intratibially [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Transformed Canine and Murine Mesenchymal Stem Cells as a Model for Sarcoma with Complex Genomics

Franceschini

Verbruggen

Tryfonidou

et al. 2021

Cancers

View full text Add to dashboard Cite

Sarcomas are rare mesenchymal tumors with a broad histological spectrum, but they can be divided into two groups based on molecular pathology: sarcomas with simple or complex genomics. Tumors with complex genomics can have aneuploidy and copy number gains and losses, which hampers the detection of early, initiating events in tumorigenesis. Often, no benign precursors are known, which is why good models are essential. The mesenchymal stem cell (MSC) is the presumed cell of origin of sarcoma. In this study, MSCs of murine and canine origin are used as a model to identify driver events for sarcomas with complex genomic alterations as they transform spontaneously after long-term culture. All transformed murine but not canine MSCs formed sarcomas after subcutaneous injection in mice. Using whole genome sequencing, spontaneously transformed murine and canine MSCs displayed a complex karyotype with aneuploidy, point mutations, structural variants, inter-chromosomal translocations, and copy number gains and losses. Cross-species analysis revealed that point mutations in Tp53/Trp53 are common in transformed murine and canine MSCs. Murine MSCs with a cre-recombinase induced deletion of exon 2-10 of Trp53 transformed earlier compared to wild-type murine MSCs, confirming the contribution of loss of p53 to spontaneous transformation. Our comparative approach using transformed murine and canine MSCs points to a crucial role for p53 loss in the formation of sarcomas with complex genomics.

show abstract

Section: Discussionmentioning

confidence: 99%