Attenuation of streptozotocin-induced oxidative stress in hepatic and intestinal tissues of wistar rat by methanolic-garlic extract

Kanth, V. Rajani; Reddy, P. U. M.; Raju, T.

doi:10.1007/s00592-008-0051-x

Cited by 35 publications

(13 citation statements)

References 28 publications

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“…1, consumption of garlic significantly decreased hepatic lipid peroxides compared with the control group, suggesting that garlic also showed antioxidant effect in vivo . This finding was in agreement with the results of previous studies which reported that injection of aqueous garlic extract (500 mg/kg) increased total serum antioxidant levels (Drobiova et al, 2009) and incubation of methanol garlic extract (500 mg/kg) decreased hepatic TBARS levels in STZ-treated diabetic rats (Kanth et al, 2008). In addition, we found that aged black garlic was more effective in reduction of hepatic TBARS levels than garlic.…”

Section: Discussionsupporting

confidence: 93%

Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus

et al. 2009

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Hyperglycemia in the diabetic state increases oxidative stress and antioxidant therapy can be strongly correlated with decreased risks for diabetic complications. The purpose of this study is to determine antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes. The antioxidant activity of garlic and aged black garlic was measured as the activity in scavenging free radicals by the trolox equivalent antioxidant capacity (TEAC) assay. Three week-old db/db mice were fed AIN-93G diet or diet containing 5% freeze-dried garlic or aged black garlic for 7 weeks after 1 week of adaptation. Hepatic levels of lipid peroxides and activities of antioxidant enzymes were measured. TEAC values of garlic and aged black garlic were 13.3 ± 0.5 and 59.2 ± 0.8 µmol/g wet weight, respectively. Consumption of aged black garlic significantly decreased hepatic thiobarbituric acid reactive substances (TBARS) level compared with the garlic group which showed lower TBARS level than control group (p<0.05). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of garlic and aged black garlic group were significantly elevated compared to the control group. Catalase (CAT) activity of aged black garlic group was increased compared with the control group. These results show that aged black garlic exerts stronger antioxidant activity than garlic in vitro and in vivo, suggesting garlic and aged black garlic, to a greater extent, could be useful in preventing diabetic complications.

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Section: Discussionsupporting

confidence: 93%

Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus

et al. 2009

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“…A broad range of antioxidant properties have been attributed to allicin, including direct scavenging of free radicals, maintenance of glutathione and the endogenous antioxidant redox balance, as well as the enhanced expression of the antioxidant enzymes glutathione peroxidase, glutathione reductase, SOD and CAT ( 13 ). Furthermore, allicin can suppress the production of certain inflammatory cytokines that have been implicated in the pathogenesis of diabetic complications, including tumor necrosis factor-α and transforming growth factor-1 ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

“…As a traditional Chinese herbal medicine, garlic has been used to treat a range of diseases for thousands of years. Allicin is one of the main active components in garlic and exerts several therapeutic effects, such as promoting insulin sensitivity ( 6 , 7 ), decreasing blood glucose levels ( 8 , 9 ), regulating lipid metabolism ( 10 , 11 ), reducing homocysteine levels ( 12 ), attenuating superoxide production ( 13 ) and limiting inflammation and fibrogenesis. Given the role of oxidative stress within the etiology and pathogenesis of diabetic complications, the present study utilized an in vitro model of diabetes-associated oxidative stress using aortic endothelial cells cultured under high-glucose/hypoxic conditions to clarify the possible mechanism of allicin on diabetic macrovascular complications.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress

Wang

Liu

Liang

et al. 2015

Experimental and Therapeutic Medicine

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This study was designed to explore the protective effect of allicin on aortic endothelial cell injury induced by high glucose/hypoxia and to investigate the corresponding mechanisms. The primary-cultured murine aortic endothelial cells were subcultured. The third passage of cells was adopted and randomly divided into five groups: The normal group (NG), the mannitol group (MG), the high-glucose/hypoxia group (HG), the allicin group (AG) and the protein kinase C (PKC) inhibitor group (GG). The general morphology was observed under an inverted phase-contrast microscope and cell viability was assessed using the MTT assay. Intracellular reactive oxygen species (ROS) levels in the endothelial cells were quantified using dihydroethidium staining. The levels of 8-hydroxydeoxyguanosine (8-OHdG), nuclear factor-κB (NF-κB), NADPH oxidase 4 (Nox4) and hypoxia-inducible factor-1α (HIF-1α) and the activity of PKC were measured using ELISA. A quantitative polymerase chain reaction (qPCR) was adopted to evaluate the mRNA expression of Nox4, HIF-1α and NF-κB. The altered cell morphology observed in HG was notably ameliorated in the AG and GG. The protein levels of 8-OHdG, NF-κB, Nox4, HIF-1α and PKC in the HG were higher than those in the other groups. Furthermore, the cell viability in the AG was significantly increased and the protein levels of 8-OHdG, NF-κB, Nox4, HIF-1α and PKC were significantly decreased compared with those in the HG. The ROS production was found to be increased in the HG cells, while there was a significant decrease in the AG cells. These data indicate that allicin exerts a protective effect against high glucose/hypoxia-induced injury in aortic endothelial cells through its antioxidative action, which may involve the inhibition of the PKC pathway and regulation of HIF-1α.

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“…Recent studies also reveal the beneficial effects of garlic or its preparations in combating various diseases. Hypo-lipidaemic, anti-atherosclerotic, anticoagulant, antidiabetic, antihypertensive, antimicrobial, anticancer, antidote, hepatoprotective, and immunomodulatory activities of garlic are now well- established from animal and human studies ( Rajani Kanth et al, 2008 ). The salutary effects of garlic in Type-I and Type-II diabetes are also well established ( Padiya et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Garlic and Resveratrol Attenuate Diabetic Complications, Loss of β-Cells, Pancreatic and Hepatic Oxidative Stress in Streptozotocin-Induced Diabetic Rats

Kaur¹,

Padiya²,

Adela³

et al. 2016

Front. Pharmacol.

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The study was aimed at finding the effect of garlic and resveratrol on loss of β-cells and diabetic complication in streptozotocin (STZ)-induced Type-I diabetic rats. Rats were injected with single dose STZ (50 mg/kg, i.p.) for induction of type 1 diabetes (Dia) and compared with control group. Rats from third (Dia+Gar), fourth (Dia+Resv), and fifth (Dia+Met) groups were fed raw garlic homogenate (250 mg/kg/day), resveratrol (25 mg/kg/day), and metformin (500 mg/kg/day) orally, respectively, for a period of 4 weeks. Diabetic group had decreased serum insulin and hydrogen sulfide levels along with increased blood glucose and glycated hemoglobin, triglyceride, uric acid, and nitric oxide levels. Significant (p < 0.05) increase in pancreatic and hepatic TBARS, conjugated dienes, nitric oxide, and AGE level and significant (p < 0.05) decrease in SOD, catalase, H2S, GSH level were observed in diabetic group. Administration of garlic, resveratrol, and metformin significantly (p < 0.05) normalized most of the altered metabolic and oxidative stress parameters as well as histopathological changes. Administration of garlic, resveratrol, and metformin in diabetic rat decreases pancreatic β-cell damage and hepatic injury. Our data concluded that administration of garlic showed more promising effect in terms of reducing oxidative stress and pathological changes when compared to resveratrol and metformin groups.

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Attenuation of streptozotocin-induced oxidative stress in hepatic and intestinal tissues of wistar rat by methanolic-garlic extract

Cited by 35 publications

References 28 publications

Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus

Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus

Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress

Garlic and Resveratrol Attenuate Diabetic Complications, Loss of β-Cells, Pancreatic and Hepatic Oxidative Stress in Streptozotocin-Induced Diabetic Rats

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