AtRD22 and AtUSPL1, Members of the Plant-Specific BURP Domain Family Involved in Arabidopsis thaliana Drought Tolerance

Harshavardhan, Vokkaliga T.; Sơn, Lê Văn; Seiler, Christiane; Junker, Astrid; Weigelt‐Fischer, Kathleen; Klukas, Christian; Altmann, Thomas; Sreenivasulu, Nese; Bäumlein, Helmut; Kuhlmann, Markus

doi:10.1371/journal.pone.0110065

Cited by 78 publications

(46 citation statements)

References 68 publications

(69 reference statements)

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“…To unravel the mechanism by which HbEBP1 OE enhances drought resistance in Arabidopsis , expression of the drought-responsive genes RD22 and RD29a was examined (Yamaguchi-Shinozaki and Shinozaki, 1993b, 1994; Msanne et al, 2011; Harshavardhan et al, 2014). Leaves were detached from 2-week-old seedlings and subjected to dehydration for 4 h. Then the expression of dehydration-responsive genes was detected.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis

et al. 2016

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Rubber trees are economically important tropical tree species and the major source of natural rubber, which is an essential industrial material. This tropical perennial tree is susceptible to cold stress and other abiotic stresses, especially in the marginal northern tropics. Recent years, the genome sequencing and RNA-seq projects produced huge amount of sequence data, which greatly facilitated the functional genomics study. However, the characterization of individual functional gene is in urgent demands, especially for those involved in stress resistance. Here we identified and characterized the rubber tree gene ErbB-3 binding protein 1, which undergoes changes in expression in response to cold, drought stress and ABA treatment. HbEBP1 overexpression (OE) in Arabidopsis increased organ size, facilitated root growth and increased adult leaf number by delaying the vegetative-to-reproductive transition. In addition, HbEBP1 OE enhanced the resistance of the Arabidopsis plants to freezing and drought stress, demonstrating that this gene participates in the regulation of abiotic stress resistance. RD29a, RD22 and CYCD3;1 expression was also greatly enhanced by HbEBP1 OE, which explains its regulatory roles in organ size and stress resistance. The regulation of drought stress resistance is a novel function identified in plant EBP1 genes, which expands our understanding of the roles of EBP1 gene in response to the environment. Our results provide information that may lead to the use of HbEBP1 in genetically engineered crops to increase both biomass and abiotic stress resistance.

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Section: Resultsmentioning

confidence: 99%

Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis

et al. 2016

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“…; Harshavardhan et al. ). This clustering indicates that the three B. rapa BURP proteins and the ATUSPL1 protein have similar functions.…”

Section: Discussionmentioning

confidence: 99%

“…The protein products of AtRD22 and AtUSPL1, both members of the thale cress BURP domain‐containing gene family, suppress drought stress response (Harshavardhan et al. ). ADR6 is a soybean BURP protein that is down‐regulated by auxin (Datta et al.…”

Section: Introductionmentioning

confidence: 99%

The divergence and positive selection of the plant‐specific BURP‐containing protein family

Wang

Zhu

et al. 2015

Ecology and Evolution

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BURP domain‐containing proteins belong to a plant‐specific protein family and have diverse roles in plant development and stress responses. However, our understanding about the genetic divergence patterns and evolutionary rates of these proteins remain inadequate. In this study, 15 plant genomes were explored to elucidate the genetic origins, divergence, and functions of these proteins. One hundred and twenty‐five BURP protein‐encoding genes were identified from four main plant lineages, including 13 higher plant species. The absence of BURP family genes in unicellular and multicellular algae suggests that this family (1) appeared when plants shifted from relatively stable aquatic environments to land, where conditions are more variable and stressful, and (2) is critical in the adaptation of plants to adverse environments. Promoter analysis revealed that several responsive elements to plant hormones and external environment stresses are concentrated in the promoter region of BURP protein‐encoding genes. This finding confirms that these genes influence plant stress responses. Several segmentally and tandem‐duplicated gene pairs were identified from eight plant species. Thus, in general, BURP domain‐containing genes have been subject to strong positive selection, even though these genes have conformed to different expansion models in different species. Our study also detected certain critical amino acid sites that may have contributed to functional divergence among groups or subgroups. Unexpectedly, all of the critical amino acid residues of functional divergence and positive selection were exclusively located in the C‐terminal region of the BURP domain. In conclusion, our results contribute novel insights into the genetic divergence patterns and evolutionary rates of BURP proteins.

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“…RD22 has also been reported to be involved in Arabidopsis drought tolerance and ABA-mediated stress responses in grape [33,34]. These results suggested that ABA-mediated stress responses might be activated for stress tolerance of transgenic plants.…”

Section: Some Unknown Function Genes and Several Abiotic Stress Respomentioning

confidence: 84%

Overexpression of <em>AmRosea1 </em>Gene Confers Drought and Salt Tolerance in Rice

Dou

Fan

Yang

et al. 2016

Preprint

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Ectopic expression of the MYB transcription factor of AmROSEA1 from Antirrhinum majus has been reported to change anthocyanin and other metabolites in several species. In this study, we found that overexpression of AmRosea1 significantly improved the tolerance of transgenic rice to drought and salinity stress. Transcriptome analysis revealed that a considerable amount of stress-related genes were affected by exogenous AmRosea1 during both drought and salinity stress treatments. These affected genes are involved in stress signal transduction, the hormone signal pathway, ion homeostasis and the enzymes that remove peroxides. This work suggests that the AmRosea1 gene is a potential candidate for genetic engineering of crops.

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AtRD22 and AtUSPL1, Members of the Plant-Specific BURP Domain Family Involved in Arabidopsis thaliana Drought Tolerance

Cited by 78 publications

References 68 publications

Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis

Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis

The divergence and positive selection of the plant‐specific BURP‐containing protein family

Overexpression of <em>AmRosea1 </em>Gene Confers Drought and Salt Tolerance in Rice

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