Atomic Force Microscopy Meets Biophysics, Bioengineering, Chemistry, and Materials Science

Toca-Herrera, José L.

doi:10.1002/cssc.201802383

Cited by 23 publications

(18 citation statements)

References 87 publications

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“…As optical techniques have been important for the biological decoding of cellular processes, the study of mechanical properties has required the development of new techniques. Among these, atomic force microscopy (AFM) has been one of the most important [3,4,5,6]. AFM allows nondestructive measurements, together with good lateral resolution, in the natural setting of biological samples.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy

Iturri

Weber

Moreno-Cencerrado

et al. 2019

IJMS

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Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young’s modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0–6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment.

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Section: Introductionmentioning

confidence: 99%

Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy

Iturri

Weber

Moreno-Cencerrado

et al. 2019

IJMS

Self Cite

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“…Indeed, the atomic force microscopy is a well-recognized tool for nanoscale materials patterning and engineering, 48,49 just as much as for bioengineering, investigations in biochemistry and cell biology. 50,51 Inkjet printing is a cost-efficient nanofabrication method, especially for the labscale prototyping. 52,53 It typically operates with a picolitre droplet volume and 10-20 mm spatial resolution 52 of the droplet depositing.…”

Section: Discussionmentioning

confidence: 99%

Inkjet assisted fabrication of planar biocompatible memristors

et al. 2019

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“…Tuning of the tips was done automatically through instrument software (NanoScope version 8.15). Both topographic [14] and phase [24] images of 40 µm x 40 µm (1600 µm 2 ) scan areas were recorded. The root-mean-square (RMS) surface roughness was calculated as the standard deviation of all the height values within an image area using Nanoscope Analysis_v1.40 image processing software.…”

Section: Atomic Force Microscopy (Afm) Imaging and Surface Roughness mentioning

confidence: 99%

“…Lens polymers are often imaged by AFM in tapping mode, in which a cantilever tip oscillates at its natural resonance frequency and repeatedly contacts the surface for short increments of time as the cantilever is rastered over the analysis area. Toca-Herrera describes the technique in detail [14]. Briefly, the vibrating tip repeatedly nears, taps, and retreats from the surface, which changes the amplitude of vibration dependent upon surface topography.…”

Section: Introductionmentioning

confidence: 99%

Development and Preliminary Evaluation of a System to Rapidly Measure Coefficient of Friction on Soft Contact Lenses

Hook¹,

Lusignan²,

Wyglądacz³

et al. 2019

IJOVS

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This study was undertaken to 1. develop an apparatus to rapidly measure coefficient of friction (COF) on soft contact lenses; 2. determine if COFs measured on two daily-disposable lens models before and after wear are consistent with changes in lens surface morphology observed in parallel atomic force microscopy (AFM) images. Methods: A stress rheometer was adapted to measure COF on a soft contact lens by custom fabrication of a rapid-mount sample stage for increased throughput. Five subjects were randomly assigned to wear daily disposable nesofilcon A and delefilcon A contact lenses bilaterally for 4 hours, after which time lenses were removed. Static and kinetic COFs of lenses worn on left eyes was measured, while lenses worn on right eyes were imaged in parallel by AFM in tapping mode. Root mean square (RMS) surface roughness was calculated for all lenses to determine the effect of wear on surface topography. Results: Both static and kinetic COFs measured on unworn delefilcon A silicone hydrogel lenses were greater than on nesofilcon A traditional hydrogel lenses. Static COF on nesofilcon A increased significantly after wear, while kinetic COF trended higher but did not change significantly. Similarly, static COF on delefilcon A also increased significantly after wear, and kinetic COF trended higher but did not change significantly, both remaining greater than on worn nesofilcon A. Parallel AFM analysis demonstrated that nesofilcon A lenses are smoother than are delefilcon A out of the package. Both lenses attracted deposits during wear, but the nesofilcon A surface was less altered by on-eye wear than was the delefilcon A surface. Conclusion: A system to rapidly measure static and kinetic COFs was successfully developed. Static and kinetic COFs measured on delefilcon A were greater than on nesofilcon A lenses. More deposits and greater surface roughness were observed after wear on delefilcon A relative to nesofilcon A. Parallel AFM images of worn and unworn lenses were not predictive of measured COFs, but increased roughness visible by AFM was consistent with observed increases in COF, although not all increases were statistically significant.

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Atomic Force Microscopy Meets Biophysics, Bioengineering, Chemistry, and Materials Science

Cited by 23 publications

References 87 publications

Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy

Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy

Inkjet assisted fabrication of planar biocompatible memristors

Development and Preliminary Evaluation of a System to Rapidly Measure Coefficient of Friction on Soft Contact Lenses

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