Atividade das glicosidases na presença de chá verde e de chá preto

Pereira, Luciana Lopes Silva; Souza, Stefânia P. de; Silva, M. C.; Carvalho, G. A.; Santos, Custódio Donizete dos; Corrêa, Angelita Duarte; Abreu, Celeste Maria Patto de

doi:10.1590/s1516-05722010000400017

Cited by 8 publications

(7 citation statements)

References 6 publications

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“…Our results demonstrate a reduction in the activity of enzyme inhibition after exposure to simulated gastric fluid, except for sample D. These results corroborate Pereira et al,(2010) who worked with green and black teas, observed inhibition of alphaamylase 42.19 and 73.44%, respectively, and absence of inhibition after simulation with gastric fluid. Freitas et al,(2014) assessed the activity of enzyme inhibition of extracts of commercial teas Syzygium sp front of the alpha-amylase by different extraction methods (infusion, decoction and maceration) and different proportions (1:20, 1:50 and 1 : 100) obtained significant inhibition of the enzyme before exposure to fluid, with an average of 92.84%.…”

Section: Resultssupporting

confidence: 91%

Inhibition of alpha-amylase by insulin plant (Myrcia sphaerocarpa DC) extracts: an alternative for the treatment of diabetes mellitus?

Oliveira¹,

Pereira²

2015

J App Pharm Sci

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Diabetes mellitus is an important medical-economical-social problem requiring up search for less costly alternatives for treatment, such as use of medicinal plants. Of the species cited in ethnobotanical studies, the "insulin plant" (Myrcia sphaerocarpa DC) appears among those used by diabetics. The objective of this work was to evaluate the inhibitory activity of alpha-amylase by aqueous extracts of "insulin plant", prepared by different methods at a proportion of 1:200 (w:v), for information about the alleged hypoglycemic effect. For this, five samples of commercially teas "insulin plant" were obtained; aqueous extracts were prepared; and thereafter, they were tested for inhibition of alpha-amylase, before and after exposure to simulated gastric fluid. Results demonstrated before exposure to gastric fluid, a significant inhibition of enzyme activity (greater than 50%) in the samples A and C, using the decoction and sample E in all methods of extraction. Comparing methods of extraction, the decoction was more effective. After exposure to gastric fluid, there was a reduction in the activity of enzyme inhibition, suggesting negative influence of low pH on the properties of the inhibitor. These results do not elucidate the mechanism of the hypoglycemic action reported in folk medicine and literature, but indicate a promising path.

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Section: Resultssupporting

confidence: 91%

Inhibition of alpha-amylase by insulin plant (Myrcia sphaerocarpa DC) extracts: an alternative for the treatment of diabetes mellitus?

Oliveira¹,

Pereira²

2015

J App Pharm Sci

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“…The enzymes used in these assays were porcine pancreatic lipase (EC 3.1.1.3) type II (Sigma), porcine pancreatic α-amylase (EC 3.2.1.1) type VI B (Sigma), and porcine pancreatic trypsin (EC 3.4.21.4) (Merck). Α-glucosidase (EC 3.2.1.20) was obtained from the fresh porcine duodenum as per Pereira et al (2010) [15].…”

Section: Kinetic Assays With Digestive Enzymes: With and Without Gast...mentioning

confidence: 99%

Toxic or medicinal? New approach to the pharmacological and toxicological properties of Echinodorus macrophyllus leaves

Braga,

Eleutério,

Cesar

et al. 2024

Sci. Plena

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Echinodorus macrophyllus contains some phytochemicals that can induce toxicity but are also promising as ingredients in products to promote and maintain health, improve the immune system, and prevent diseases. In this study, the aqueous and ethanolic extracts of leaves of this species were characterized by high-performance liquid chromatography, investigated in vitro regarding their modulating action on snake venom enzymes, and evaluated in digestive enzyme inhibition assays. The extracts inhibited the phospholipase activity induced by Bothrops jararacussu and B. moojeni venoms. The hemolytic activity induced by Crotalus durissus terrificus venom was potentiated by 30-73% by the ethanolic extract at the ratios analyzed. The thrombolytic activity induced by B. moojeni venom was inhibited by 51% and 34% by the aqueous and ethanolic extracts at a ratio of 1:5 (w:w), respectively. The clotting time induced by B. moojeni and Lachesis muta muta venoms was prolonged after incubation with either extract. The extracts also exerted antigenotoxic action on DNA fragmentation induced by L. muta muta venom. Both extracts lowered the enzymatic activity of α-glucosidases, and the ethanolic extract inhibited α-amylases. The inhibition of digestive enzymes suggests the extracts could decrease calorie availability from carbohydrate intake. Results confirm the potential therapeutic use of E. macrophyllus in the prevention and treatment of hemostatic changes, since the proteins of venoms inhibited by it exhibit high homology with human proteins involved in various pathophysiological processes, such as inflammatory responses and blood coagulation.

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“…After that, the nucleoids remained in a neutralization solution (0.4 M Tris-HCl, pH 7.4) for 25 minutes and then were fixed with ethanol and visualized after staining with propidium iodide using epifluorescence microscopy. Analysis of nucleotide fragmentation levels was performed according to classes described by Collins et al, [52] , with some adaptations: class 0, damages � [54] α-Amylase Activity…”

Section: Comet Assaymentioning

confidence: 99%

Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

Braga

Abreu

Trento

et al. 2019

Chemistry & Biodiversity

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The aqueous and ethanolic extracts of Lippia sidoides Cham. were chemically characterized and tested for their action on enzymes involved in processes such as inflammation, blood coagulation, and digestion. Both extracts potentiated the activity of phospholipases A2 present in the venom of Bothrops atrox in 12 % and completely inhibited the hemolysis induced by B. jararacussu and B. moojeni venoms in the proportions between 1 : 0.5 and 1 : 5 (venom/extracts (w/w)). They inhibited the thrombolysis induced by B. moojeni (10 to 25 %), potentiated the thrombolysis induced by the Lachesis muta muta venom (30 to 80 %), prolonged the coagulation time induced by B. moojeni and L. muta muta venoms, and presented antigenotoxic action. Both extracts reduced the activity of α‐glycosidases, the aqueous extract inhibited lipases, and the ethanolic extract inhibited α‐amylases. The results demonstrate the modulatory action of the extracts on proteases, phospholipases, and digestive enzymes. In addition, the rich phenolic composition of these extracts highlights their potential for nutraceutical use.

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Atividade das glicosidases na presença de chá verde e de chá preto

Cited by 8 publications

References 6 publications

Inhibition of alpha-amylase by insulin plant (Myrcia sphaerocarpa DC) extracts: an alternative for the treatment of diabetes mellitus?

Inhibition of alpha-amylase by insulin plant (Myrcia sphaerocarpa DC) extracts: an alternative for the treatment of diabetes mellitus?

Toxic or medicinal? New approach to the pharmacological and toxicological properties of Echinodorus macrophyllus leaves

Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis

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