Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex.Lindl. (Blue Vanda): An in vitro protocol for an endangered orchid

Roy, Aniruddha; Patel, Rajesh; Patel, V.V.; Sajeev, Sudha; Deka, Bidyut C.

doi:10.1016/j.scienta.2011.01.023

Cited by 55 publications

(44 citation statements)

References 22 publications

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“…In Vanda coerulea, Roy et al (2011) verified that 0.85 mg L -1 BA favored shoot formation and that the addition of 1 mg L -1 of the auxin naphthaleneacetic acid (NAA) together with this BA concentration did not alter shoot production. Hossain et al (2010) reported that in Cymbidium giganteum Wall.…”

Section: Eff Ects Of Culture Media Sucrose and Growth Regulators On mentioning

confidence: 98%

“…Suzuki et al (2009), upon studying the germination of Hadrolaelia tenebrosa, also obtained higher germinability when seeds were inoculated on KN, which has lower nitrogen concentrations. On the other hand, Roy et al (2011) reported that MS was more effective than KN and VW for the germination of Vanda coerulea Griff. ex Lindl.…”

Section: Germination and Initial Developmentmentioning

confidence: 99%

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Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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-This investigation aimed at studying the germination and initial growth of Alatiglossum fuscopetalum (Hoehne) Baptista under in vitro conditions. The eff ects of Murashige/Skoog (MS and ½MS), Knudson (KN), and Vacin/Went (VW) culture media on the in vitro germination of A. fuscopetalum were analyzed. The infl uence of these media on the multiplication and initial growth of 90-day-old seedlings, and of diff erent sucrose, benzyladenine [BA] and indole-butyric acid [AIB] concentrations on the multiplication and growth of 120-day-old plants were also evaluated. The results revealed that ½MS was the most appropriate medium for germination. Regarding the growth of 90-day-old seedlings, MS provided the best outcome. The most eff ective medium for the multiplication and growth of 120-day-old plants was ½MS. Sucrose ranging from 1% to 3% favored plant growth. BA at 1 mg L -1 and IBA at 2 mg L -1 were the best growth regulator treatments for shoot proliferation and root growth, respectively.

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Section: Eff Ects Of Culture Media Sucrose and Growth Regulators On mentioning

confidence: 98%

Section: Germination and Initial Developmentmentioning

confidence: 99%

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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“…The germination potential of mature and immature seed samples were scored under a light microscope. Seed germination percentage was calculated by the following formula: % seed germination=(Number of seeds forming spherule ÷ Total number of seeds)×100 (Roy et al 2011). The germination potential for independent seed samples was established concurrent to culture initiation.…”

Section: Seed Culture and Germinationmentioning

confidence: 99%

Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity

Balilashaki

Gantait

Naderi

et al. 2015

Physiol Mol Biol Plants

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We explored the influence of pollination season and maturity of capsule on post-pollination capsule formation and in vitro asymbiotic seed germination, respectively. Three Phalaenopsis orchid hybrids, namely, 'Athens', 'Moscow' and 'Lusaka' flowers were artificially self-pollinated during winter, spring, summer and fall seasons and the impact of the pollination seasons was evident during capsule formation. It was observed that winter was the most suitable season for pollination of all the three Phalanaeopsis hybrids resulting in 80-88 % capsule formation. During summer, the pollination success rate was 24-28 %, but resulted in successful capsule formation. Season of pollination further delimited the germination efficiency of seeds harvested from capsules of variable maturity levels. Invariably, seeds collected from winterpollinated capsules performed best in germination compared to other seasons, for instance, 'Moscow' seeds took less than 14 days to germinate from capsules developed following winter-pollination. Regarding the influence of capsule maturity on seed germination, we observed that seeds derived from 5-month mature capsules, invariably took least time to germinate than that of the 3-month or 7-month in all three hybrids, e.g., for 'Moscow' it was 13.9 days with a maximum of 90.3 % germination.

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“…Ainda, com relação à influência do pH na formação da parte aérea, em investigação realizada em híbridos de Cymbidium, foi evidenciado efeito significativo do pH na formação de folhas, as quais exerceram influência direta nesta variável (SILVA et al, 2006). Vale salientar que pHs entre 5,8 e 6,0 apresentam os melhores resultados culturais para o crescimento da parte aérea para os representantes da família Orchidaceae, sejam eles terrestres, litófitos ou epífitos (BESSON et al, 2010;CUNHA et al, 2011;GALE et al, 2010;KEEL;ZETTLER;KAPLIN, 2011;LIAO et al, 2011;ROY et al, 2011;SOARES et al, 2010;SOUTO et al, 2010).…”

Section: Growth Of Oncidium Flexuosum Sims (Orchidaceae)unclassified

Concentração de Sacarose e Índices de pH no Crescimento In Vitro de Oncidium flexuosum SIMS. (ORCHIDACEAE)

2016

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O objetivo do trabalho foi avaliar o efeito de diferentes concentrações de sacarose e índices de pH no crescimento in vitro de Oncidium flexuosum. As plântulas foram cultivadas em meio de cultura MS/2 para testar os efeitos da sacarose (0, 15, 30, 45 e 60 g.L-1) e pH (5,0, 5,8, e 6,6) no seu desenvolvimento, em função das variáveis: altura da parte aérea (APA), número de raízes (NR), comprimento da maior raiz (CMR), número de folhas (NF) e massa fresca (MF). As concentrações de 15 e 30 g.L-1, independentemente do pH, foram as mais eficientes para a cultura in vitro da espécie, pois promoveram um aumento no comprimento das raízes, altura e massa fresca das plântulas. O pH foi significativo somente em combinação com 15 g.L-1de sacarose para o comprimento da maior raiz e massa fresca.

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Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex.Lindl. (Blue Vanda): An in vitro protocol for an endangered orchid

Cited by 55 publications

References 22 publications

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity

Concentração de Sacarose e Índices de pH no Crescimento In Vitro de Oncidium flexuosum SIMS. (ORCHIDACEAE)

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