Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity

Balilashaki, Khosro; Gantait, Saikat; Naderi, Roohangiz; Vahedi, Maryam

doi:10.1007/s12298-015-0309-z

Cited by 11 publications

(5 citation statements)

References 18 publications

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“…requires 7–9 months to mature (Ávila‐Díaz et al, 2009 ). As noted here and in other studies, the age of seeds can have a strong effect on germination (Tsuchiya, 1954 ; Balilashaki et al, 2015 ; Zhang et al, 2015 ; Kendon et al, 2017 ). Seeds harvested too early may germinate but then fail to develop further, whereas seeds harvested when fully mature can have reduced germination rates.…”

Section: Discussionsupporting

confidence: 80%

The challenges of growing orchids from seeds for conservation: An assessment of asymbiotic techniques

et al. 2022

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Lewis Knudson first successfully germinated orchid seeds asymbiotically on artificial medium in 1922. While many orchid species have since been grown asymbiotically, the tremendous variation in how species respond to artificial medium and growth conditions ex situ has also become apparent in the past century. In this study, we reviewed published journal articles on asymbiotic orchid seed germination to provide a summary of techniques used and to evaluate if these differ between terrestrial and epiphytic species, to identify areas where additional research is needed, and to evaluate whether asymbiotic germination could be used more often in ex situ conservation. We found articles reporting successful asymbiotic germination of 270 species and 20 cultivars across Orchidaceae. Researchers often used different techniques with epiphytic versus terrestrial species, but species‐specific responses to growth media and conditions were common, indicating that individualized protocols will be necessary for most species. The widespread success in generating seedlings on artificial media suggests that asymbiotic techniques should be another tool for the conservation of rare orchid species. Further advances are needed in understanding how to introduce mycorrhizae to axenically grown orchids and to maximize the viability of seedlings reintroduced into natural habitats to fully utilize these methods for conservation.

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Section: Discussionsupporting

confidence: 80%

The challenges of growing orchids from seeds for conservation: An assessment of asymbiotic techniques

et al. 2022

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“…Lots of propagation techniques were developed for Phalaenopsis species and their hybrids through the in vitro culture from various explants, including leaf segments/tissue [3], flower stalk nodes [4], callus and protocorm-like bodies [5], flower stalks [6], and seeds [7, 8]. However, propagation through in vitro seed culture has not yet been achieved in P. amboinensis orchid.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm

Utami

Hariyanto

2019

Scientifica

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Phalaenopsis amboinensis, an epiphytic orchid, has a great potential for commercial exploitation in the cut-flower industry. It is difficult to propagate vegetatively as it naturally grows slowly. Therefore, there is a need to improve the propagation methods to avoid endangering its natural populations. The objective of this study was to identify the best medium and organic supplements for seed germination and plantlets development of P. amboinensis. Seeds from 4-month-old hand-pollinated orchids were sown on different strengths of MS and VW culture media: Murashige and Skoog (MS), 1/2 MS, Vacin and Went (VW), and 1/2 VW. Optimum seed germination, i.e., 90.7%, was achieved on VW medium. VW medium was suitable for seedling formation and allowed 51.4% of seedling development from protocorm within 10 weeks of culture. When 15% (v/v) coconut water was added together with banana homogenate (10 g·L−1) to the VW medium, the plantlets grew to the highest length and had the highest dry weight (62.1 mm and 15.5 g, respectively). The roots and leaves of the plantlets grew vigorously in this medium. Plants regenerated via in vitro seed germination processes were successfully acclimatized in greenhouse conditions, and the survival rate was more than 85%.

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“…According to McCall and Primack (1992) environmental circumstances considerably affect the fruit/seed development that was noticeably manifested from the outcomes of the current experiment. Further, the advantageous impact of cooler seasons on efficacious post-pollination capsule development coroborated by the studies of Vaknin et al (1996) and Balilashaki et al (2015), who reported that the frequency of fruit set was lesser in summer in comparison to winter ones in Loranthus acaciae Zucc. (Loranthaceae) and Phalaenopsis respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Seeds were isolated by softly teasing the surface sterilized slitcapsules with scalpel and cultured on different media: the surface of half-strength Murashige and Skoog (1962) (MS), Vacin-Went (VW) (Vacin and Went, 1949) or modified (supplemented with pepton) Chen medium (Chen et al, 1999), solidified with 0.25% (w/v) Gelrite (Balilashaki et al, 2015). The media pH were corrected to 5.7 before autoclaving at 121 °C and 105 kg cm −2 for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Few reports are attainable on seed germination of Phalaenopsis (Mweetwa et al, 2008;Balilashaki et al, 2015) and no report ensues on the asymbiotic or symbiotic seed germination requirements of Phalaenopsis 'Dublin', an admired hybrid of Middle East. Considering the importance of asymbiotic seed germination system for mass propagation, the present study aimed at optimizing a reproducible system of in vitro seed germination of Phalaenopsis 'Dublin' and in due course the influence of pollination time and type of medium on capsule formation and seed germination frequency, respectively, were assessed.…”

Section: Introductionmentioning

confidence: 99%

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Asymbiotic Germination of <i>Phalaenopsis</i> cv. ‘Dublin’ Seeds in Relation to Pollination Months and Nutrient Media

et al. 2015

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Orchids are one of the most popular flowers in the world and Phalaenopsis genus shares maximum sales in global floral industry among them. Successful pollination, followed by capsule formation and in vitro seed germination, is a powerful tool for mass propagation of orchids. In the present study, flowers of Phalaenopsis cv. ‘Dublin’ were self-pollinated artificially during the months of November, December, January, February, March and April. The influence of pollination month over capsule formation was assessed and it was observed that pollination during December resulted as maximum with 86% capsule formation, followed by 72% during January. However, only 40% successful capsule formation occurred following pollination during April. Seeds from mature capsules developed through artificial pollination in different months were cultured on three different culture media (modified Chen, ½Murashige Skoog and Vacin-Went medium) for in vitro germination. A maximum of 97% germination of seeds (collected from capsules developed through pollination in January) was recorded on modified Chen medium. Nevertheless, the earliest initiation of germination occurred around 12 days of culture when seeds were harvested from capsules (derived through pollination in December) and inoculated on modified Chen medium. The plantlets developed following germination were rooted on ½MS supplemented with 2.5 g/l activated charcoal. Subsequently, well-rooted plantlets were acclimatized on potting mixture that contained cocopeat, charcoal, industrial cartridge and the bites of yonolit (1:1:2:4; v/v) with a very high rate of success. A seed culture protocol that crops a bulk number of seedlings in a short time span is advantageous and the present report eventually provides such simple yet proficient method that can be further tested.

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Capsule formation and asymbiotic seed germination in some hybrids of Phalaenopsis, influenced by pollination season and capsule maturity

Cited by 11 publications

References 18 publications

The challenges of growing orchids from seeds for conservation: An assessment of asymbiotic techniques

The challenges of growing orchids from seeds for conservation: An assessment of asymbiotic techniques

In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm

Asymbiotic Germination of <i>Phalaenopsis</i> cv. ‘Dublin’ Seeds in Relation to Pollination Months and Nutrient Media

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