Associations between metabolites of aromatic compounds in bile and the occurrence of hepatic lesions in English sole (Parophrys vetulus) from Puget Sound, Washington

Relationships between Hepatic Neoplasms and Related Lesions and Exposure to Toxic Chemicals in Marine Fish from the U.S. West Coast

“…2) that incorporates neoplasm prevalence, sediment conmiinant levels, interactions among different classes ofchemicals, and fish size (an indirect measure of fish age) from multiple studies in Puget Sound (30,31 (32)] and prevalences ofliver lesions in sole from multiple sampling sites (29,33). Although the positive correlation between these bile metabolites and neoplasms has received much attention, equally strong correlations exist between these metabolites and prevalences of megalocytic hepatosis and foci of cellular alteration (Table 3).…”

Hepatic metabolism of contaminants in the terapontid fish, yellowtail trumpeter (Amniataba caudavittata Richardson)

“…However, it is noted that, within the B[a]P-injected groups there was a high interindividual variability in the data sets when compared to the control and BNF-injected groups. This variability may be due to the dose-response being nonlinear, and to the rates at which individuals take up and metabolise aromatic compounds (Krahn et al, 1986;Vignier et al, 1996). The high interindividual variability may have masked statistical differences between the treatment groups, and makes EROD activity induction an unsuitable biomarker in this species.…”

“…This offers the advantage of a particularly sensitive detection of a group of metabolites originating from a common parent compound (Lin et al, 1996). For example, nearly all ''B[a]P-type'' metabolites fluoresce at 380/430 nm (Lin et al, 1996) and ''pyrene-type'' metabolites fluoresce at 340/380 nm (Krahn et al, 1986). The concentrations of PAH bile metabolites are reported as metabolite equivalents to their respective standards, representing the amount of a metabolite that would be present if the group of metabolites originated from a parent compound (Krahn et al, 1986).…”

“…For example, nearly all ''B[a]P-type'' metabolites fluoresce at 380/430 nm (Lin et al, 1996) and ''pyrene-type'' metabolites fluoresce at 340/380 nm (Krahn et al, 1986). The concentrations of PAH bile metabolites are reported as metabolite equivalents to their respective standards, representing the amount of a metabolite that would be present if the group of metabolites originated from a parent compound (Krahn et al, 1986). Neither B[a]P-type nor pyrene-type metabolites were expected to occur in the BNF-treated fish.…”

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

“…Hence, hepatocytic CYP1A induction, accumulation of PAH metabolites in bile and elevated liver DNA adducts represent a chain of events that, although partly separated in time, are tightly interrelated from a mechanistic point of view. These three biomarkers have, therefore, received much attention and represent valuable biomarkers of PAH-exposure and effect in fish [159][160][161][162]. Analogous with several studies on mammals, responses of these core biomarkers of oil exposure have been associated with genotoxic effects such as liver neoplasia [163].…”