Association of Three Plasmid-Encoded spv Genes Among Different Salmonella Serotypes Isolated from Different Origins

Derakhshandeh, Abdollah; Firouzi, R.; Khoshbakht, Rahem

doi:10.1007/s12088-012-0316-5

Cited by 19 publications

(11 citation statements)

References 27 publications

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“…There are also other studies that have found lower frequencies for this gene in strains of avian origin (Okamoto et al 2009, Derakhshandeh et al 2013, Moussa et al 2013. It is possible that the presence of this gene is related to the host from which the sample was isolated (Amini et al 2010).…”

Section: Discussionmentioning

confidence: 86%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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RESUMO.-[Detecção de genes associados à virulência em cepas de Salmonella Enteritidis isoladas de frangos na região sul do Brasil.] Salmonella spp. estão entre os principais agentes causadores de doenças transmitidas por alimentos, e o sorovar Salmonella Enteritidis é o mais frequentemente isolado no mundo. A virulência de Salmonella spp. e a sua interação com o hospedeiro são processos complexos que envolvem fatores de virulência para sobreviver às defesas do hospedeiro. O objetivo deste estudo foi detectar genes de virulência em cepas de S. Enteritidis isoladas a partir de fontes avícolas no sul do Brasil. Ensaios de PCR foram desenvolvidos para a detecção de nove genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH are considered the main agents of foodborne disease and Salmonella Enteritidis is one of the most frequently isolated serovars worldwide. The virulence of Salmonella spp. and their interaction with the host are complex processes involving virulence factors to overcome host defenses. The purpose of this study was to detect virulence genes in S. Enteritidis isolates from poultry in the South of Brazil. PCR-based assays were developed in order to detect nine genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH and spvC) associated with the virulence in eighty-four isolates of S. Enteritidis isolated from poultry. The invA, hilA, sivH, sefA and avrA genes were present in 100% of the isolates; lpfA and sopE were present in 99%; agfA was present in 96%; and the spvC gene was present in 92%. It was possible to characterize the isolates with four different genetic profiles (P1, P2, P3 and P4), as it follows: P1, positive for all genes; P2, negative only for spvC; P3, negative for agfA; and P4, negative for lpfA, spvC and sopE. The most prevalent profile was P1, which was present in 88% of the isolates. Although all isolates belong to the same serovar, it was possible to observe variations in the presence of these virulence-associated genes between different isolates. The characterization of the mechanisms of virulence circulating in the population of Salmonella Enteritidis is important for a better understanding of its biology and pathogenicity. The frequency of these genes and the establishment of genetic profiles can be used to determine patterns of virulence. These patterns, associated with in vivo studies, may help develop tools to predict the ability of virulence of different strains.

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Section: Discussionmentioning

confidence: 86%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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“…Subsequent uptake of apoptotic macrophages by surrounding macrophages, facilitates cell to cell spread of Salmonella spp. (Guiney and Lesnick, 2005;Derakhshandeh et al, 2013). Consequently, it potentiates the systemic spread of the pathogen instead of causing a self limited gastroenteritis.…”

Section: The Pathogenesis Tissue Invasion and Immune Responsesmentioning

confidence: 99%

Salmonella spp. in poultry: a constant challenge and new insights

Velhner

Milanov

Kozoderović

2018

J Hellenic Vet Med Soc

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ABSTRACT. The knowledge about virulence mechanisms, resistance to antimicrobial agents and the biofilm formation ability of Salmonella spp. in poultry industry has been expanded over the years. However, in spite of the research efforts and significant investments to improve management systems in poultry industry, it has become evident that none of the methods applied in all stages of food production chain are 100% effective in eliminating Salmonella spp. Different serovars are manifesting different mechanisms of invasiveness which depend on their ability to invade lower zones of the lamina propria, their ability to gain accesses to parenchymatous organs and survive in macrophages. The ubiquitous nature of Salmonella spp. due to their adaptation to animal and plant hosts, as well as their survival in hostile environments and their enhanced capacity to produce biofilms, contribute to a long lasting contamination of the environment, feed and animals. The emergency and spread of antimicrobial resistances in Salmonella spp. raise additional concerns.

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“…The invA gene is a conserved virulence gene in nearly all Salmonella species; therefore, the right candidate for the detection of Salmonella using different PCR methods [20]. The study also selected the Salmonella plasmid virulence C (spvC) gene, which is of virulence plasmid for detecting strains associated with non-typhoid bacteremia [21]. Salmonella has plasmids with a highly conserved locus known as spv operon.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Salmonella Typhimurium from village chickens based on invA and spvC genes

2020

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Aim: This study aimed to identify Salmonella enterica serovars by polymerase chain reaction (PCR) based on virulence genes invasion A (inv A) and Salmonella plasmid virulence C (spvC). Materials and Methods: DNA extraction of eight bacteria isolates was done using the PowerSoil® DNA Isolation Kit. The amplification of invA and spvC genes was done using conventional PCR. The positive PCR products were purified using the GeneJET Purification Kit and then sequenced using ABI 3730 XL automated genetic analyzer. The sequences obtained were compared for similarities with other Salmonella serovars deposited on the NCBI GenBank using BLASTN. Results: Four out of eight samples were amplified by primers FS139/RS141 that target invA gene with products of about 284 bp, and three out of four of the same invA positive samples were also amplified by primers FSPV-1/RSPV-2 targeting spvC with a product of about 571 bp. One sample was not amplified by primers FSPV-1/RSPV-2 as it lacked virulence plasmid. Analysis of sequences indicated 100% homology with closely related serovars of S. enterica subspecies enterica serovar Typhimurium. Conclusion: Salmonella Typhimurium that contained invA and spvC genes are pathogenic and virulent strains.

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Association of Three Plasmid-Encoded spv Genes Among Different Salmonella Serotypes Isolated from Different Origins

Cited by 19 publications

References 27 publications

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Salmonella spp. in poultry: a constant challenge and new insights

Molecular identification of Salmonella Typhimurium from village chickens based on invA and spvC genes

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