Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen

Suarez, Alexandra Usuga; Rojano, Benjamín Alberto; Betancur, Giovanni Restrepo

doi:10.1071/rd17044

Cited by 19 publications

(23 citation statements)

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“…In a previous study, we have reported a relation between nonsynonymous SNPs of the CRISP‐3 gene and post‐thawing semen quality of Colombian Creole stallions. Different homozygous and heterozygous genotypes had higher or lower results for motility, kinetics, sperm vitality, abnormal morphology and membrane functional integrity of spermatozoa (Usuga et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…All analyses were conducted using SAS version 9.2. software (SAS Inst. Inc., Cary, NC; Usuga et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that CRISP-3 protein in stallion SP suppresses polymorphonuclear sperm binding, suggesting that this protein regulates sperm elimination from the female reproductive tract (Doty et al, 2011), while a proteomic study has shown a positive association of the CRISP-3 protein with the stallion semen freezability (Jobim et al, 2011). Moreover, nonsynonymous polymorphism of CRISP-3 gene has been found to correlate with first-cycle conception rates in stallions (Hamann et al, 2007), and we have recently reported a relationship between CRISP-3 genotype and seminal plasma protein and post-thawing semen quality in stallions (Usuga, Rojano, & Restrepo, 2018). Additionally, a recent genomewide association study for semen quality traits included the CRISP-3 gene among the candidate genes influencing stallion fertility (Gottschalk, Metzger, Martinsson, Sieme, & Distl, 2016).…”

Section: Introductionmentioning

confidence: 86%

“…Primers were designed using publicly available equine genomic sequences ( AJ459965 ; Giese et al, ). Primer sequences and PCR conditions were defined according to Usuga et al (). PCR products were verified by electrophoresis (2% agarose gel).…”

Section: Methodsmentioning

confidence: 99%

“…Primer sequences and PCR conditions were defined according to Usuga et al (2018). PCR products were verified by electrophoresis (2% agarose gel).…”

Section: Snp Evaluationmentioning

confidence: 99%

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Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Betancur

Rojano

Suarez

2018

Reprod Domestic Animals

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Cysteine-rich secretory protein-3 (CRISP-3) and some of its nonsynonymous polymorphism have been related to the fertility and freezability of stallion semen; however, the role of the CRISP-3 gene and its seminal plasma protein in the raw semen quality is still unknown. The aim of this study was to evaluate the relationship of CRISP-3 with semen quality in stallions. DNA was obtained from blood samples of 100 stallions, from which 30 stallions were randomly selected to obtain 60 ejaculates. Through PCR amplification and sequencing, the variation of four nonsynonymous SNPs from CRISP-3 was identified and haplotypes were derived. Semen quality was assessed through the total motility (MOT), sperm vitality (SV), normal morphology (NM), functional integrity of membrane (MI) and a seminal quality index (SQi). CRISP-3 protein content of seminal plasma (SP) was determined by ELISA. The effect of the genotype, the haplotype and the concentration of the CRISP-3 protein on the seminal quality were evaluated through generalized linear models and linear regression analyses. Homozygous genotypes for SNP1, SNP2 and SNP3 and the heterozygous genotype for SNP4 showed a positive effect on seminal quality. Different haplotypes with positive effect on MOT, SV, NM, MI and SQi were identified. The allelic substitution analysis resulted in positive regression coefficients for MOT (SNP2) and MI (SNP2 and SNP3). A high level of CRISP-3 resulted in a higher MOT and SQi. It is concluded that the quality of stallion semen is influenced by the genotype of CRISP-3 and the concentration of CRISP-3 protein in SP.

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Section: Discussionmentioning

confidence: 99%

“…All analyses were conducted using SAS version 9.2. software (SAS Inst. Inc., Cary, NC; Usuga et al, 2018).…”

Section: Discussionmentioning

confidence: 99%