Comparative proteomic analysis of seminal plasma proteins in relation to freezability of Dezhou donkey semen

Li, Jingling; Li, Min; Ji, Chuanliang; Li, Xuexian; Li, Haijing; Liu, Guiqin; Wang, Yantao; Liu, Guangyuan; Wang, Tao; Che, Xiaonan; Lei, Chuzhao; Dang, Ruihua; Zhao, Fuwei

doi:10.1016/j.anireprosci.2021.106794

Cited by 11 publications

(6 citation statements)

References 44 publications

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“…This study is a continuation of our recently published report on the differences in donkey seminal plasma protein profile in relation to GF and PF semen (Yu et al, 2021). In this study, the same GF ( n = 4) and PF ( n = 4) semen samples were used to analyse the seminal plasma and spermatozoa (Yu et al, 2021). The experiment was conducted using procedures consistent with the Regulations of the Administration of Affairs Concerning Experimental Animals (Ministry of Science and Technology, Beijing, China, 2004), and the research was approved by the Institutional Animal Care and Use Committee of Northwest A & F University.…”

Section: Methodssupporting

confidence: 67%

“…This study is a continuation of our recently published report on the differences in donkey seminal plasma protein profile in relation to GF and PF semen (Yu et al, 2021). In this study, the same GF (n = 4) and PF (n = 4) semen samples were used to analyse the seminal plasma and spermatozoa (Yu et al, 2021). The experiment was conducted using procedures consistent with the Regulations Semen samples were obtained from Dezhou jacks routinely used in AI (artificial insemination) programs on a local farm at which there was development of about 100 jacks in Liaocheng City, Shandong, China.…”

Section: Sample Collectionsupporting

confidence: 59%

Section: Sample Collectionmentioning

confidence: 82%

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Metabolomic profiling of Dezhou donkey seminal plasma related to freezability

Wang

Zhong

et al. 2022

Reprod Domestic Animals

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Donkeys are indispensable livestock in China because they have transport function and medicinal value. With the popularization of artificial insemination on donkeys, semen cryopreservation technology has gradually become a research hotspot.Seminal plasma is a necessary medium for transporting sperm and provides energy and nutrition for sperm. Seminal plasma metabolites play an important role in the process of sperm freezing, and also have an important impact on sperm motility and fertilization rate after freezing and thawing. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was used to compare the metabolic characteristics of seminal plasma of high freezability (HF) and low freezability (LF) male donkeys. We identified 672 metabolites from donkey seminal plasma, of which 33 metabolites were significantly different between the two groups. Metabolites were

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Section: Methodssupporting

confidence: 67%

“…This study is a continuation of our recently published report on the differences in donkey seminal plasma protein profile in relation to GF and PF semen (Yu et al, 2021). In this study, the same GF (n = 4) and PF (n = 4) semen samples were used to analyse the seminal plasma and spermatozoa (Yu et al, 2021). The experiment was conducted using procedures consistent with the Regulations Semen samples were obtained from Dezhou jacks routinely used in AI (artificial insemination) programs on a local farm at which there was development of about 100 jacks in Liaocheng City, Shandong, China.…”

Section: Sample Collectionsupporting

confidence: 59%

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Metabolomic profiling of Dezhou donkey seminal plasma related to freezability

Wang

Zhong

et al. 2022

Reprod Domestic Animals

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“…This shows that (i) spermatogenesis is a complex process involving controlled regulation of different transcriptional factors and that (ii) long non-coding RNAs (lncRNA) are crucial for proper spermatogenesis and sperm function. Several other studies have reported the importance of performing transcriptomic analysis in identifying genes that are necessary for normal spermatogenesis [48][49][50].…”

Section: Transcriptomicsmentioning

confidence: 99%

Omics and Male Infertility: Highlighting the Application of Transcriptomic Data

Omolaoye

Kandasamy

et al. 2022

Life

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Male infertility is a multifaceted disorder affecting approximately 50% of male partners in infertile couples. Over the years, male infertility has been diagnosed mainly through semen analysis, hormone evaluations, medical records and physical examinations, which of course are fundamental, but yet inefficient, because 30% of male infertility cases remain idiopathic. This dilemmatic status of the unknown needs to be addressed with more sophisticated and result-driven technologies and/or techniques. Genetic alterations have been linked with male infertility, thereby unveiling the practicality of investigating this disorder from the “omics” perspective. Omics aims at analyzing the structure and functions of a whole constituent of a given biological function at different levels, including the molecular gene level (genomics), transcript level (transcriptomics), protein level (proteomics) and metabolites level (metabolomics). In the current study, an overview of the four branches of omics and their roles in male infertility are briefly discussed; the potential usefulness of assessing transcriptomic data to understand this pathology is also elucidated. After assessing the publicly obtainable transcriptomic data for datasets on male infertility, a total of 1385 datasets were retrieved, of which 10 datasets met the inclusion criteria and were used for further analysis. These datasets were classified into groups according to the disease or cause of male infertility. The groups include non-obstructive azoospermia (NOA), obstructive azoospermia (OA), non-obstructive and obstructive azoospermia (NOA and OA), spermatogenic dysfunction, sperm dysfunction, and Y chromosome microdeletion. Findings revealed that 8 genes (LDHC, PDHA2, TNP1, TNP2, ODF1, ODF2, SPINK2, PCDHB3) were commonly differentially expressed between all disease groups. Likewise, 56 genes were common between NOA versus NOA and OA (ADAD1, BANF2, BCL2L14, C12orf50, C20orf173, C22orf23, C6orf99, C9orf131, C9orf24, CABS1, CAPZA3, CCDC187, CCDC54, CDKN3, CEP170, CFAP206, CRISP2, CT83, CXorf65, FAM209A, FAM71F1, FAM81B, GALNTL5, GTSF1, H1FNT, HEMGN, HMGB4, KIF2B, LDHC, LOC441601, LYZL2, ODF1, ODF2, PCDHB3, PDHA2, PGK2, PIH1D2, PLCZ1, PROCA1, RIMBP3, ROPN1L, SHCBP1L, SMCP, SPATA16, SPATA19, SPINK2, TEX33, TKTL2, TMCO2, TMCO5A, TNP1, TNP2, TSPAN16, TSSK1B, TTLL2, UBQLN3). These genes, particularly the above-mentioned 8 genes, are involved in diverse biological processes such as germ cell development, spermatid development, spermatid differentiation, regulation of proteolysis, spermatogenesis and metabolic processes. Owing to the stage-specific expression of these genes, any mal-expression can ultimately lead to male infertility. Therefore, currently available data on all branches of omics relating to male fertility can be used to identify biomarkers for diagnosing male infertility, which can potentially help in unravelling some idiopathic cases.

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“…Sperm cryopreservation is extensively used in infertility treatment and fertility preservation in cancer patients, which has consequently resulted in millions of live births from these patients ( Yu et al, 2021 ). Sperm cryopreservation can be performed by either slow freezing or vitrification.…”

Section: Potential Counter Measures To the Effect Of Microgravity On ...mentioning

confidence: 99%

Effects of space flight on sperm function and integrity: A systematic review

et al. 2022

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With the advancement in space exploration and the intention to establish an inhabitable human settlement on Mars, it is important to investigate the effects of exposure to space/microgravity and the associated radiations on procreation. Sperm function and integrity are fundamental to male reproduction and can potentially be affected by the environmental changes experienced in space. Therefore, this study was conducted to systematically gather, filter, and collate all the relevant information on the effects of spaceflight on male reproductive parameters and functions. A search was performed utilizing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Data were extracted from the major electronic databases including PubMed, and other credible literature sources. MeSH search terms that were employed included “spermatozoa”, “microgravity”, and “ionizing radiation”. The literature search did not discriminate against papers published before a certain date due to the very limited number of articles available. However, there was a restriction on the male gender and language (English). The parameters included in this study are sperm motility, total sperm count, sperm DNA fragmentation hormonal levels and testicular histology. Following a comprehensive literature search, a total of 273 articles were retrieved and screened, 252 articles were excluded due to the irrelevance to the topic, duplication, and non-original articles. A total of 21 articles met the inclusion criteria and are included in the current study. Findings from these studies showed that sperm motility was decreased after exposure to microgravity and ionizing radiation. Total sperm count was also found to be reduced by microgravity only. Sperm DNA fragmentation was increased by both ionizing radiation and microgravity. Testosterone levels and testicular weight were also decreased by microgravity. Although there is a dearth in the literature regarding the effects of microgravity and ionizing radiation on male reproductive parameters, the available findings showed that exposure to microgravity poses a risk to male reproductive health. Therefore, it is essential to develop countermeasures to either manage, treat, or prevent these consequential adverse effects. Hence, this review also highlights some potential countermeasure approaches that may mitigate the harmful effects of microgravity and associated exposures on male reproductive health.

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Comparative proteomic analysis of seminal plasma proteins in relation to freezability of Dezhou donkey semen

Cited by 11 publications

References 44 publications

Metabolomic profiling of Dezhou donkey seminal plasma related to freezability

Metabolomic profiling of Dezhou donkey seminal plasma related to freezability

Omics and Male Infertility: Highlighting the Application of Transcriptomic Data

Effects of space flight on sperm function and integrity: A systematic review

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