Association of Spot14α Gene Polymorphisms with Body Weight in the Chicken

Abstract

In mammals, thyroid hormone responsive Spot14 (THRSP) is a small acidic protein that responds to thyroid hormone stimulation and, therefore, is thought to play a role in growth. The current study was designed to investigate the associations of Spot14alpha gene polymorphisms on chicken growth and body composition traits. The Northeast Agricultural University Resource Population (NEAURP) was used in the present study. The NEAURP was established by crossing broiler sires, derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content, with Baier layer dams, a local Chinese breed. The F(1) birds were intercrossed to produce the F(2) population. Body weight and body composition traits were measured in the F(2) population. Polymorphisms of the gene were detected between parental lines by DNA sequencing. Primers were designed according to the chicken Spot14alpha gene (AY568628). The PCR-RFLP and PCR-length polymorphisms methods were then developed to genotype polymorphisms in the NEAURP. The A213C and 9 bp insertion-deletion of the Spot14alpha gene in the F(2) population was found to be associated with BW, which implied that Spot14alpha gene or a tightly linked gene had an important effect on growth in the chicken.

Polymorphism identification in the goat <i>THRSP</i> gene and association analysis with growth traits

“…Wang et al (2004) reported THRSPα locus was associated with abdominal fat traits in a broiler × Leghorn resource population. The A213C and 9 bp insertion-deletion of exon 1 in the THRSP gene was found to be associated with body weight in the chicken (Cao et al 2007). This supports the notion that further investigation of THRSP variation in different goat breeds is needed.…”

Prolactin, dopamine receptor D1 and Spot14α polymorphisms affect production traits of Hungarian Yellow hens

“…In the Hungarian Yellow population, the A allele was more favourable, and indicated an increase in body weight. The A213C SNP had significant (P<0.05) effects on body weight from 6 to 12 wk of age in an F 2 broiler×layer cross; however, the polymorphism acted in a completely different way, since the C allele was clearly beneficial for producing heavier chickens (Cao et al, 2007). Based on these apparently contradictory results, a non-direct effect of the SNP was suggested, where the actual causative mutation was closely linked to A213C, and is in inverse linkage in the two populations.…”

“…With slight modifications, the genotyping methods for the 24 bp insertion in PRL, the G123A locus in DRD1, the A213C locus in Spot14α, and the A570C SNP in IGF1 were used as described by Jiang et al (2005), Xu et al (2010), Cao et al (2007), and Zhou et al (2005), respectively (Table 1). Primers were designed and RFLP was constructed for non-synonymous SNPs: the G645T in IGFBP2, and the A370G in SST (Table 1).…”

Comparative Study on Various Growth Performances of Korean Oge and White Leghorn Chickens

“…However, in a recent study, Insulin-like growth factor 1 is an essential growth factor required for rapid postnatal growth. Insulin-like growth factor 1 associated genes such as SPOT14 homolog also plays an important role on body weight (Cao et al, 2007;Marquez et al, 2010).…”

“…It was tested for environ-mental stress effect for their body weight growth (Gross and Siegel, 1980). WL chickens are also very good resource for genetic studies such as quantitative trait locus (QTL) (Tsudzuki et al, 2007;Ankra-Badu et al, 2010;Podisi et al, 2011), genetic differentiation (Tadano et al, 2011), and gene polymorphism (Cao et al, 2007).…”