Prolactin, dopamine receptor D1 and Spot14α polymorphisms affect production traits of Hungarian Yellow hens

Tempfli, Károly; Konrád, Szilárd; K, Gaál; Pongrácz, László; Papp, Ágnes

doi:10.1016/j.livsci.2015.01.012

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Allele G was identified by 198-and 117-bp-long fragments, allele T was represented by a 315-bp-long product. Only allele G was found in Hungarian Yellow hens in a previous study (Tempfli et al, 2015).…”

Section: Resultsmentioning

confidence: 69%

Genotyping of four loci in Hungarian Yellow and broiler chickens

Szalai

Tempfli

Lencsés-Varga

et al. 2019

Acta Veterinaria Hungarica

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Traditional selection has led to remarkable differences in allele frequencies among various chicken breeds. Indigenous and broiler-type chicken populations were genotyped for polymorphisms in thyroid hormone responsive Spot14α, prolactin (PRL), IGF-binding protein 2 (IGFBP2), and somatostatin (SST) genes in order to determine potential utilisation type-associated allele frequencies. Significant (P < 0.05) differences were detected between Hungarian Yellow and broiler populations for Spot14α, PRL, and IGFBP2 allele frequencies, whereas the same SST allele (A) was fixed in both groups. In this study, the most significant associations (P < 0.05) were found between the IGFBP2 genotypes and the measured traits (body weight, carcass weight, breast muscle weight with or without skin, breast muscle weight as a percentage of carcass weight) in the broiler population. The results can be applied for the evaluation of polymorphism effects in the analysed populations; however, contradictory allele effects in different breeds and hybrids indicate the need for cautious marker utilisation in selection programmes.

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Section: Resultsmentioning

confidence: 69%

Genotyping of four loci in Hungarian Yellow and broiler chickens

Szalai

Tempfli

Lencsés-Varga

et al. 2019

Acta Veterinaria Hungarica

Self Cite

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“…Dopamine promotes prolactin (PRL) secretion via DRD1 at the hypothalamic level, while DRD2 inhibits the secretion of PRL ( Youngren et al, 1998 ; Al et al, 2003 ). In a study of the production traits of the Hungarian yellow chicken, DRD1 was associated with egg strength and body weight ( Tempfli et al, 2015 ). The expression of DRD1 undoubtedly increases PRL secretion, resulting in an increase in brooding behavior frequency.…”

Section: Discussionmentioning

confidence: 99%

“…Tempfli et al found that DRD1 promotes ovarian development by PRL and induces SYF differentiation in hens. Xu et al reported that polymorphisms in DRD1 affect the egg- laying performance of hens and that its haplotypes are significantly associated with some egg production traits in chickens ( Xu et al, 2010 ; Tempfli et al, 2015 ). Wang et al reported that DRD1 may be a target gene for improving the characteristics of duck reproduction ( Wang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals miR-302a-3p Affects Granulosa Cell Proliferation by Targeting DRD1 in Chickens

et al. 2022

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Egg production is an important economic trait in laying chickens as higher yields bring higher profits. Small yellow follicle (SYFL) development is a key determinant of chicken reproductive performance; however, the majority of SYFLs are not selected during the process of chicken reproduction and thus, atresia occurs. Although there have been numerous omic studies focused on egg production, the molecular mechanisms involved are still not well-understood. In this study, we used high-throughput technology to analyze the differences between the SYFL mRNA transcriptomes of high– (H) and low–egg-yielding (L) Taihang layer hens, with the aim of identifying the potential candidate genes involved in controlling the rate of egg production. We constructed six cDNA libraries, three from H and three from L Taihang hens and then performed high-throughput sequencing. Comparison of the H and L groups showed 415 differentially expressed genes (DEGs). In the high-yield group, 226 were upregulated and 189 were downregulated. Differentially enriched biological functions and processes were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis. Ten of the candidate DEGs we identified (DRD1, MC5R, PCK1, CTSA, TGFBR3, AGO4, SLIT2, RGS1, SCNN1B, and ZP3) have been identified in previous studies as being involved in the development of small yellow follicles. DRD1 was significantly enriched in the gap junction pathway, which is an important pathway in chicken granulosa cells (GCs) to pass nutrition to an oocyte. Homology analysis showed that DRD1 was highly conserved in numerous species, indicating that it may be a productive target for improving egg production. Evidence from bioinformatics analysis revealed that gga-miR-302a-3p putatively targets the 3′UTR region of DRD1. We then identified the functions of gga-miR-302a-3p in follicular granulosa cell proliferation by targeting DRD1. RT-qPCR analysis showed that DRD1 and miR-302a-3p expression were inversely related in the SYLs of high and low egg-yielding chickens. Luciferase assays showed that miR-302a-3p targets the 3′UTR of DRD1, and overexpression of miR-302a-3p significantly inhibits the expression of DRD1 in chicken GCs (p < 0.01). Functional experiments revealed that by targeting DRD1, miR-302a-3p acts as an inhibitor of GC proliferation. Taken together, we concluded that miR-302a-3p affects chicken GC proliferation by targeting DRD1. Our data expanded the knowledge base of genes whose functions are important in egg production and the molecular mechanisms of high-yield egg production in chicken small yellow follicles.

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